Box C/D snoRNP catalysed methylation is aided by additional pre-rRNA base-pairing

2′‐O‐methylation of eukaryotic ribosomal RNA (r)RNA, essential for ribosome function, is catalysed by box C/D small nucleolar (sno)RNPs. The RNA components of these complexes (snoRNAs) contain one or two guide sequences, which, through base‐pairing, select the rRNA modification site. Adjacent to the guide sequences are protein‐binding sites (the C/D or C′/D′ motifs). Analysis of >2000 yeast box C/D snoRNAs identified additional conserved sequences in many snoRNAs that are complementary to regions adjacent to the rRNA methylation site. This ‘extra base‐pairing’ was also found in many human box C/D snoRNAs and can stimulate methylation by up to five‐fold. Sequence analysis, combined with RNA–protein crosslinking in Saccharomyces cerevisiae, identified highly divergent box C′/D′ motifs that are bound by snoRNP proteins. In vivo rRNA methylation assays showed these to be active. Our data suggest roles for non‐catalytic subunits (Nop56 and Nop58) in rRNA binding and support an asymmetric model for box C/D snoRNP organization. The study provides novel insights into the extent of the snoRNA–rRNA interactions required for efficient methylation and the structural organization of the snoRNPs.

• Publication year

  2011

• Venue

  EMBO Journal

• Publication date

  2011-05-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/emboj.2011.148PMID 21556049PMCID 3116282
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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