Isolation and characterization of lipolytic Pseudomonas spp . from oil contaminated water samples

Vivek Vishwe,Shrikant P. Gajbhiye,S. Vaidya,A. Chowdhary

Published 2015 in Unknown venue

ABSTRACT

Oil contaminated water samples collected from different areas in Pune were screened for the selective isolation of lipolytic Pseudomonas spp. Screening medium containing Tributyrin (1% v/v) and Rhodamine B was used for the specific isolation of lipolytic Pseudomonas spp. from oil contaminated water samples. Lipolytic bacteria showing zone of clearance around the colonies were selected for the present study. Isolated bacteria were identified upto genus level with the help of morphological and biochemical testing by following Bergey’s manual. Spectrophotometric Lipase assay showed that out of 49 lipolytic Pseudomonas spp., WP23 exhibits the maximum Lipase activity of about 45.4733 U/ml.

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    2015

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    Open on Semantic Scholar

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