The genetic population structure and temporal genetic stability of gilthead sea bream Sparus aurata populations in the Aegean and Ionian Seas, using microsatellite DNA markers.

We examined 662 gilthead sea bream Sparus aurata from wild samples of the species in the Aegean and Ionian Seas, using 20 EST-linked microsatellite markers, in three multiplex panels, as well as seven anonymous loci. Most of the markers were revealed to be highly polymorphic. We found low genetic differentiation between the sampling stations/areas with total FST 0.002 (P < 0.05). Based on comparison of five temporal samples, our results indicate genetic data consistency over time for all tested samples, pointing to stable populations, despite reported repeated escape events. Our results confirm the genetic population structure previously observed in these specific areas, using by far more markers than in previous studies in both coding and non-coding DNA loci. The limited genetic structure and the temporal genetic stability indicate neither major genetic differentiation of local populations by geographic isolation nor influence from anthropogenic factors. These results provide a baseline for future reference in any management programme of both wild and farmed population of S. aurata as well as of other aquaculture species with a potential introgression among farmed and wild populations.

• Publication year

  2019

• Venue

  Journal of Fish Biology

• Publication date

  2019-04-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1111/jfb.13932PMID 30746701
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Implications of Sea Bream and Sea Bass Escapes for Sustainable Aquaculture Management: A Review of Interactions, Risks and Consequences

  2018cited by this paper
• Performance and precision of double digestion RAD (ddRAD) genotyping in large multiplexed datasets of marine fish species.

  2018cited by this paper
• Performance and precision of double digestion RAD (ddRAD) genotyping in large multiplexed datasets of marine fish species.

  2018cited by this paper
• Genetic assessment of three gilthead sea bream (Sparus aurata L.) populations along the Spanish coast and of three broodstocks managements

  2016cited by this paper
• Simple sequence repeats (SSRs) markers in fish genomic research and their acceleration via next-generation sequencing and computational approaches

  2016cited by this paper
• Clumpak: a program for identifying clustering modes and packaging population structure inferences across K

  2015cited by this paper
• SNP genotyping and population genomics from expressed sequences – current advances and future possibilities

  2015cited by this paper
• Microsatellites markers to depict the reproductive and genetic patterns of farmed gilthead seabream (Sparus aurata): illustration by a case study on mass spawning

  2014cited by this paper
• Challenges in analysis and interpretation of microsatellite data for population genetic studies

  2014cited by this paper
• Evidence for ‘escape through spawning’ in large gilthead sea bream Sparus aurata reared in commercial sea-cages

  2013cited by this paper
• Genetic variation in farmed populations of the gilthead sea bream Sparus aurata in Greece using microsatellite DNA markers

  2012cited by this paper
• GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update

  2012cited by this paper
• Low levels of population genetic structure in the gilthead sea bream, Sparus aurata, along the coast of Italy

  2012cited by this paper
• Population genetic structure of reared and wild gilthead sea bream (Sparus aurata) in the Adriatic Sea inferred with microsatellite loci

  2011cited by this paper
• Exploring neutral and adaptive processes in expanding populations of gilthead sea bream, Sparus aurata L., in the North-East Atlantic

  2011cited by this paper
• In silico mining and characterization of simple sequence repeats from gilthead sea bream (Sparus aurata) expressed sequence tags (EST-SSRs); PCR amplification, polymorphism evaluation and multiplexing and cross-species assays.

  2011cited by this paper
• A review on population characteristics of gilthead seabream (Sparus aurata).

  2010cited by this paper
• Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows

  2010cited by this paper
• Variation in anonymous and EST-microsatellites suggests adaptive population divergence in turbot

  2010cited by this paper
• [Strong genetic differentiation of the gilthead sea bream Sparus aurata (L., 1758) between the two western banks of the Mediterranean].

  2009cited by this paper
• Genetic comparison of temporal replicates of gilthead sea bream (Sparus aurata) from two Tyrrhenian coastal lagoons characterized by different management

  2009cited by this paper
• Microsatellite variability of wild and farmed populations of Sparus aurata.

  2009cited by this paper
• To what extent do microsatellite markers reflect genome‐wide genetic diversity in natural populations?

  2008cited by this paper
• genepop’007: a complete re‐implementation of the genepop software for Windows and Linux

  2008cited by this paper
• Possible influence of reared gilthead sea bream (Sparus aurata, L.) on wild stocks in the area of the Messolonghi lagoon (Ionian Sea, Greece)

  2007cited by this paper
• Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment

  2007cited by this paper
• At-sea distribution and scale-dependent foraging behaviour of petrels and albatrosses: a comparative study.

  2007cited by this paper
• Temporal genetic variation in populations of Diplodus sargus from the SW Mediterranean Sea

  2007cited by this paper
• Genetic structure of gilthead seabream, Sparus aurata, in the Central Mediterranean Sea

  2006cited by this paper
• The importance of identifying spatial population structure in restocking and stock enhancement programmes

  2006cited by this paper
• The Atlantic-Mediterranean transition: discordant genetic patterns in two seabream species, Diplodus puntazzo (Cetti) and Diplodus sargus (L.).

  2005cited by this paper
• Expressed sequence tag-linked microsatellites as a source of gene-associated polymorphisms for detecting signatures of divergent selection in atlantic salmon (Salmo salar L.).

  2005cited by this paper
• Detecting the number of clusters of individuals using the software structure: a simulation study

  2005cited by this paper
• FAST‐TRACK: Integrating QTL mapping and genome scans towards the characterization of candidate loci under parallel selection in the lake whitefish (Coregonus clupeaformis)

  2004cited by this paper
• Molecular systematics

  2004cited by this paper
• Genetic differentiation between populations of gilthead seabream (Sparusaurata) along the Tunisian coast

  2004cited by this paper
• Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata)

  2004cited by this paper
• Genetic differentiation of Diplodus sargus (Pisces: Sparidae) populations in the south-west Mediterranean

  2004cited by this paper
• Microsatellite markers reveal population structure in gilthead sea bream Sparus auratus from the Atlantic Ocean and Mediterranean Sea

  2004cited by this paper
• Genetic divergence and population structure of black sea bream Acanthopagrus schlegeli inferred from microsatellite analysis

  2003cited by this paper
• microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets

  2003cited by this paper
• Twelve new microsatellite markers for gilted seabream (Sparus aurata L.): characterization, polymorphism and linkage

  2003cited by this paper
• Loss of microsatellite diversity and low effective population size in an overexploited population of New Zealand snapper (Pagrus auratus)

  2002cited by this paper
• Genetic diversity of red sea bream Pagrus major in western Japan in relation to stock enhancement

  2001cited by this paper
• Genetic interactions between marine finfish species in European aquaculture and wild conspecifics

  2001cited by this paper
• Inference of population structure using multilocus genotype data.

  2000cited by this paper
• Characterization of six polymorphic microsatellite markers in gilthead seabream, Sparus aurata (Linnaeus 1758).

  1999cited by this paper
• Separating the wheat from the chaff: patterns of genetic differentiation in high gene flow species

  1998cited by this paper
• FSTAT (Version 1.2): A Computer Program to Calculate F-Statistics

  1995cited by this paper
• Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.

  1992cited by this paper
• ANALYZING TABLES OF STATISTICAL TESTS

  1989cited by this paper
• Accuracy of estimated phylogenetic trees from molecular data

  1983cited by this paper

• Genomic insights into the population structure and adaptive variation of Mullus barbatus in the Mediterranean Sea

  2025cites this paper
• Mediterranean-wide transfer of the polyopisthocotylean Sparicotyle chrysophrii between wild sparids and farmed gilthead seabream (Sparus aurata) inferred by ddRAD loci

  2024cites this paper
• Microsatellites as Molecular Markers with Applications in Exploitation and Conservation of Aquatic Animal Populations

  2023cites this paper
• Stock Discrimination of Gilthead Seabream ( Sparus aurata Linnaeus, 1758) through the Examination of Otolith Morphology and Genetic Structure

  2023influential citation
• Mediterranean Aquaculture and Genetic Pollution: A Review Combined with Data from a Fish Farm Evaluating the Ecological Risks of Finfish Escapes

  2023cites this paper
• Review on Gilthead Seabream (Sparus aurata) Aquaculture: Life Cycle, Growth, Aquaculture Practices and Challenges

  2023cites this paper
• Investigating the role of genetic variation in vgll3 and six6 in the domestication of gilthead seabream (Sparus aurata Linnaeus) and European seabass (Dicentrarchus labrax Linnaeus)

  2023cites this paper
• Investigating Genetic Diversity and Genomic Signatures of Hatchery-Induced Evolution in Gilthead Seabream (Sparus aurata) Populations

  2021cites this paper
• New genomic resources for three exploited Mediterranean fishes.

  2020cites this paper
• Multiplex microsatellite PCR panels and their application in genetic analyses of bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix)

  2020cites this paper
• Genome-wide analysis clarifies the population genetic structure of wild gilthead sea bream (Sparus aurata)

  2020cites this paper
• Genetic structuring in farmed and wild Gilthead seabream and European seabass in the Mediterranean Sea: implementations for detection of escapees

  2020cites this paper
• Development and characterization of 12 microsatellite markers for the roundtail paradisefish ( Macropodus ocellatus ) using RAD sequencing

  2020cites this paper