Partial Proteolysis of Rice Phytochrome: Comparison with Oat Phytochrome

Phytochrome was isolated from etiolated rice seedlings (Oryza sativa L.) by slight modification of the procedure for oat phytochrome. Spectral data of rice phytochrome are comparable with those of oat and rye phytochrome. Controlled proteolysis with endoproteinases Lys-C and Glu-C yielded defined fragments some of which were different for Pr and Pfr. The fragments were identified by comparison with the corresponding fragments of oat phytochrome and by comparison of the amino acid sequences of rice and oat phytochrome. Regions of the peptide chain which are differently exposed in Pr and Pfr were identified. A highly conserved sequence around residues 740-750 is discussed as candidate for an ‘‘active center’’ of signal transduction.

• Publication year

  1989

• Venue

  Zeitschrift für Naturforschung C - A Journal of Biosciences

• Publication date

  1989-09-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1515/znc-1989-9-1010PMID 2686670
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cross-reactivity of monoclonal antibodies against phytochrome from Zea and Avena

  2004cited by this paper
• The sequence of the rice phytochrome gene.

  1989cited by this paper
• The rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region.

  1989cited by this paper
• Sequence analysis of proteolytic fragments of 124-kilodalton phytochrome from etiolatedAvena sativa L.: Conclusions on the conformation of the native protein

  1988cited by this paper
• Phytochrome and photoregulation in plants

  1987cited by this paper
• A photoreversible circular dichroism spectral change in oat phytochrome is suppressed by a monoclonal antibody that binds near its N-terminus and by chromophore modification.

  1987cited by this paper
• COMPARISON OF THE PROTEIN CONFORMATIONS BETWEEN DIFFERENT FORMS (Pr AND Pfr) OF NATIVE (124 kDa) AND DEGRADED (118/114 kDa) PHYTOCHROMES FROM Avena sativa *

  1987cited by this paper
• Nucleotide and amino acid sequence of a Cucurbita phytochrome cDNA clone: identification of conserved features by comparison with Avena phytochrome.

  1986cited by this paper
• Identification of a highly conserved domain on phytochrome from angiosperms to algae.

  1986cited by this paper
• Spectral Characterization and Proteolytic Mapping of Native 120-Kilodalton Phytochrome from Cucurbita pepo L.

  1985cited by this paper
• Analysis of cloned cDNA and genomic sequences for phytochrome: complete amino acid sequences for two gene products expressed in etiolated Avena.

  1985cited by this paper
• Structure function studies on phytochrome. Identification of light-induced conformational changes in 124-kDa Avena phytochrome in vitro.

  1985cited by this paper
• Partial characterization of undegraded oat phytochrome.

  1980cited by this paper
• Action of staphylococcal proteinase on peptides of varying chain length and composition.

  1976cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Light modulation of histidine-kinase activity in bacterial phytochromes monitored by size exclusion chromatography, crosslinking, and limited proteolysis.

  2007cites this paper
• Light-induced conformational changes of cyanobacterial phytochrome Cph1 probed by limited proteolysis and autophosphorylation.

  2005cites this paper
• CHAPTER 29 – Native Pr and Pfr

  1992cites this paper
• Reduction of mRNA Level for two Nuclear Encoded LightRegulated Genes in the Barley Mutant albostrians is not Correlated with Phytochrome Content and Activity

  1991cites this paper
• Phytochrom, das Sehpigment der Pflanzen

  1991cites this paper
• Computer Analysis of Phytochrome Sequences from Five Species: Implications for the Mechanism of Action

  1990cites this paper