Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs

We generated a neonatal pig model with human infant gut microbiota (HGM) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. All the HGM-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. The gut microbiota of vaccinated pigs were investigated for effects of Lactobacillus rhamnosus GG (LGG) supplement and homotypic virulent human rotavirus (HRV) challenge. High-throughput sequencing of V4 region of 16S rRNA genes demonstrated that HGM-transplanted pigs carried microbiota similar to that of the C-section delivered baby. Firmicutes and Proteobacteria represented over 98% of total bacteria in the human donor and the recipient pigs. HRV challenge caused a phylum-level shift from Firmicutes to Proteobacteria. LGG supplement prevented the changes in microbial communities caused by HRV challenge. In particular, members of Enterococcus in LGG-supplemented pigs were kept at the baseline level, while they were enriched in HRV challenged pigs. Taken together, our results suggested that HGM pigs are valuable for testing the microbiota’s response to probiotic interventions for treating infantile HRV infection.

• Publication year

  2014

• Venue

  Gut Pathogens

• Publication date

  2014-09-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s13099-014-0039-8PMID 25349634PMCID 4209515
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Effect of human rotavirus vaccine on severe diarrhea in African infants.

  2016cited by this paper
• VIRGINIA-MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE

  2015cited by this paper
• Immune response and intestinal permeability in children with acute gastroenteritis treated with Lactobacillus rhamnosus GG: a randomized, double-blind, placebo-controlled trial.

  2014cited by this paper
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  2014influential reference
• Dual Functions of Lactobacillus acidophilus NCFM as Protection Against Rotavirus Diarrhea

  2014cited by this paper
• From meta-omics to causality: experimental models for human microbiome research

  2013cited by this paper
• A pig model of the human gastrointestinal tract

  2013cited by this paper
• Structural and functional annotation of the porcine immunome

  2013cited by this paper
• 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis.

  2012cited by this paper
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  2012cited by this paper
• An opportunistic pathogen isolated from the gut of an obese human causes obesity in germfree mice

  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2011cited by this paper
• An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea

  2011cited by this paper
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  2011cited by this paper
• Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.

  2011cited by this paper
• estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes : a systematic review and meta-analysis

  2011cited by this paper
• FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments

  2010cited by this paper
• Effect of human rotavirus vaccine on severe diarrhea in African infants.

  2010cited by this paper
• Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns

  2010cited by this paper
• Succession of microbial consortia in the developing infant gut microbiome

  2010cited by this paper
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  2010influential reference
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  2009cited by this paper
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  1996influential reference
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  1991cited by this paper
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  1964cited by this paper
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  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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  2018cites this paper
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  2018cites this paper
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  2018cites this paper
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  2016cites this paper
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  2015cites this paper
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  2015cites this paper