Active calcium responses recorded optically from nerve terminals of the frog neurohypophysis

Voltage-sensitive dyes were used to record by optical means membrane potential changes from nerve terminals in the isolated frog neurohypophysis. Following the block of voltage-sensitive Na+ channels by tetrodotoxin (TTX) and K+ channels by tetraethylammonium (TEA), direct electric field stimulation of the nerve terminals still evoked large active responses. These responses were reversibly blocked by the addition of 0.5 mM CdCl2. At both normal and low [Na+]o, the regenerative response appeared to increase with increasing [Ca++]o (0.1- 10 mM). There was a marked decrease in the size of the response, as well as in its rate of rise, at low [Ca++]o (0.2 mM) when [Na+]o was reduced from 120 to 8 mM (replaced by sucrose), but little if any effect of this reduction of [Na+]o at normal [Ca++]o. In normal [Ca++]o, these local responses most probably arise from an inward Ca++ current associated with hormone release from these nerve terminals. At low [Ca++]o, Na+ appears to contribute to the TTX-insensitive inward current.

• Publication year

  1985

• Venue

  The Journal of General Physiology

• Publication date

  1985-04-01

• Fields of study

  Biology, Medicine, Physics, Chemistry

• Identifiers
  DOI 10.1085/JGP.85.4.481PMID 2409215PMCID 2215809
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mechanism of ion permeation through calcium channels

  1984cited by this paper
• Optical recording of action potentials from vertebrate nerve terminals using potentiometric probes provides evidence for sodium and calcium components

  1983cited by this paper
• An optical determination of the series resistance in Loligo

  1983cited by this paper
• The calcium channel

  1983cited by this paper
• Optical recording of calcium action potentials from growth cones of cultured neurons with a laser microbeam.

  1981cited by this paper
• Localization of pacemaking activity in early embryonic heart monitored using voltage-sensitive dye

  1981cited by this paper
• Improvements in optical methods for measuring rapid changes in membrane potential

  1981cited by this paper
• Simultaneous optical monitoring of activity of many neurons in invertebrate ganglia using a 124-element photodiode array.

  1981cited by this paper
• Calcium component to action potentials in rat pars intermedia cells.

  1980cited by this paper
• Stimulus-secretion coupling: variations on the theme of calcium-activated exocytosis involving cellular and extracellular sources of calcium.

  1978cited by this paper
• Optical measurement of membrane potential.

  1978cited by this paper
• Separation of sodium and calcium currents in the somatic membrane of mollusc neurones. With an Appendix by Yu A. Shakhovalov

  1977cited by this paper
• Optical recording of neuronal activity in an invertebrate central nervous system: simultaneous monitoring of several neurons.

  1977cited by this paper
• The release of neural transmitter substances

  1970cited by this paper
• THE RELEASE OF NEURAL TRANSMITTER SUBSTANCES

  1969cited by this paper
• Tetrodotoxin‐resistant electric activity in presynaptic terminals

  1969cited by this paper
• The Subthreshold Potentials in a Crustacean Nerve Fibre

  1938cited by this paper

• Functional Development of the Auditory Brainstem Nuclei During Embryogenesis of the Mouse Revealed by Optical Recording With a Voltage‐Sensitive Dye

  2025cites this paper
• Functional development of olfactory nerve‐related neural circuits in the embryonic chick forebrain revealed by voltage‐sensitive dye imaging

  2022cites this paper
• Prenatal exposure to nicotine disrupts synaptic network formation by inhibiting spontaneous correlated wave activity

  2020cites this paper
• Voltage-sensitive dye recording of glossopharyngeal nerve-related synaptic networks in the embryonic mouse brainstem

  2019cites this paper
• Optical analysis of functional development of the facial motor nucleus in the embryonic rat brainstem

  2018cites this paper
• Developmental roles of the spontaneous depolarization wave in synaptic network formation in the embryonic brainstem.

  2017cites this paper
• Development of synaptic networks in the mouse vagal pathway revealed by optical mapping with a voltage‐sensitive dye

  2016cites this paper
• Optical Recording: Afferent-Evoked Activity Is Present From Early Stages Development of Functional Synaptic Connections in the Auditory System Visualized With

  2015cites this paper
• Two-Photon Excitation of Fluorescent Voltage-Sensitive Dyes: Monitoring Membrane Potential in the Infrared.

  2015cites this paper
• Positive feedback of NR2B‐containing NMDA receptor activity is the initial step toward visual imprinting: a model for juvenile learning

  2015cites this paper
• Maintenance of the large-scale depolarization wave in the embryonic chick brain against deprivation of the rhythm generator.

  2014cites this paper
• Optical survey of vagus nerve-related neuronal circuits in the embryonic rat brainstem.

  2013cites this paper
• Neural basis of imprinting behavior in chicks

  2013cites this paper
• The embryonic brain and development of vagal pathways.

  2011cites this paper
• Functional development of the vagal and glossopharyngeal nerve-related nuclei in the embryonic rat brainstem: optical mapping with a voltage-sensitive dye.

  2011cites this paper
• Voltage-sensitive dye imaging analysis of functional development of the neonatal rat corticostriatal projection

  2011cites this paper
• Demonstration of a Neural Circuit Critical for Imprinting Behavior in Chicks

  2010influential citation
• Monitoring Membrane Voltage Using Two-Photon Excitation of Fluorescent Voltage-Sensitive Dyes

  2010cites this paper
• Embryogenetic expression of glossopharyngeal and vagal excitability in the chick brainstem as revealed by voltage-sensitive dye recording.

  2007cites this paper
• Development of functional synaptic connections in the auditory system visualized with optical recording: afferent-evoked activity is present from early stages.

  2006cites this paper
• Optical imaging of large-scale correlated wave activity in the developing rat CNS.

  2005cites this paper
• Optical detection of convergent projections in the embryonic chick NTS.

  2004cites this paper
• Optical mapping reveals developmental dynamics of Mg2+-/APV-sensitive components of glossopharyngeal glutamatergic EPSPs in the embryonic chick NTS.

  2004cites this paper
• Optical Mapping of the Functional Organization of the Rat Trigeminal Nucleus: Initial Expression and Spatiotemporal Dynamics of Sensory Information Transfer during Embryogenesis

  2004cites this paper
• Depolarization waves in the embryonic CNS triggered by multiple sensory inputs and spontaneous activity: optical imaging with a voltage-sensitive dye.

  2003cites this paper
• Optical detection of developmental origin of synaptic function in the embryonic chick vestibulocochlear nuclei.

  2003cites this paper
• Optical analysis of depolarization waves in the embryonic brain: a dual network of gap junctions and chemical synapses.

  2003cites this paper
• Optical analysis of acute spontaneous epileptiform discharges in the in vivo rat cerebral cortex

  2003cites this paper
• Optical approaches to functional organization of glossopharyngeal and vagal motor nuclei in the embryonic chick hindbrain.

  2002cites this paper
• Optical imaging of spreading depolarization waves triggered by spinal nerve stimulation in the chick embryo: possible mechanisms for large‐scale coactivation of the central nervous system

  2001cites this paper
• Optical approaches to embryonic development of neural functions in the brainstem.

  2001cites this paper
• Optical detection of embryogenetic expression of vagal excitability in the rat brain stem

  2000cites this paper
• Ion channels in presynaptic nerve terminals and control of transmitter release.

  1999cites this paper
• Spatial and temporal patterns of evoked neural activity from auditory nuclei in chick brainstem detected by optical recording.

  1999cites this paper
• Optical mapping reveals the functional organization of the trigeminal nuclei in the chick embryo.

  1999cites this paper
• Optical mapping of neural network activity in chick spinal cord at an intermediate stage of embryonic development.

  1999cites this paper
• Optical Mapping of Neural Responses in the Embryonic Rat Brainstem with Reference to the Early Functional Organization of Vagal Nuclei

  1998cites this paper
• Micromolar 4-aminopyridine enhances invasion of a vertebrate neurosecretory terminal arborization: optical recording of action potential propagation using an ultrafast photodiode-MOSFET camera and a photodiode array

  1996cites this paper
• Evaluation of optimal voltage-sensitive dyes for optical monitoring of embryonic neural activity

  1995cites this paper
• Responses to glossopharyngeal stimulus in the early embryonic chick brainstem: spatiotemporal patterns in three dimensions from repeated multiple-site optical recording of electrical activity

  1995cites this paper
• Optical mapping of spontaneous electrical activity and functional organization of pacemaking area in the early embryonic chick heart

  1994cites this paper
• Optical monitoring of early appearance of spontaneous membrane potential changes in the embryonic chick medulla oblongata using a voltage-sensitive dye.

  1993cites this paper
• Aminoglycoside antibiotics block voltage-dependent calcium channels in intact vertebrate nerve terminals

  1992cites this paper
• Dendritic origin of late events in optical recordings from salamander olfactory bulb.

  1992cites this paper
• Optical mapping of the early development of the response pattern to vagal stimulation in embryonic chick brain stem.

  1991cites this paper
• Optical detection of postsynaptic potentials evoked by vagal stimulation in the early embryonic chick brain stem slice.

  1991cites this paper
• Activity-dependent modulation of nerve terminal excitation in a mammalian peptidergic system.

  1991cites this paper
• Action potential broadening and frequency-dependent facilitation of calcium signals in pituitary nerve terminals.

  1991cites this paper
• Multiple site optical recording of transmembrane voltage (MSORTV), single-unit recordings, and evoked field potentials from the olfactory bulb of skate (Raja erinacea).

  1990cites this paper
• Intraterminal recordings from the rat neurohypophysis in vitro.

  1990cites this paper
• Sodium-activated cation channels in peptidergic nerve terminals.

  1990cites this paper
• Calcium channels that are required for secretion from intact nerve terminals of vertebrates are sensitive to omega-conotoxin and relatively insensitive to dihydropyridines. Optical studies with and without voltage-sensitive dyes

  1989cites this paper
• Optical recording of voltage changes in nerve terminals and in fine neuronal processes.

  1989cites this paper
• Signals, transducers, and secretion.

  1989cites this paper
• Calcium Channels that Are Required for Secretion from Intact Nerve Terminals of Vertebrates Are Sensitive to ~0-Conotoxin and Relatively Insensitive to Dihydropyridines

  1989cites this paper
• Calcium-dependent depolarizations originating in lizard motor nerve terminals

  1989cites this paper
• Multiple‐site optical monitoring of neural activity evoked by vagus nerve stimulation in the embryonic chick brain stem.

  1989cites this paper
• Analysis of the effect of medium and membrane conductance on the amplitude and kinetics of membrane potentials induced by externally applied electric fields.

  1989cites this paper
• Calcium Channels That Are Required for Secretion from Intact Nerve Terminals of Vertebrates Are Sensitive to ~0-conotoxin and Relatively Insensitive to Dihydropyridines Optical Studies with and without Voltage-sensitive Dyes

  1989cites this paper
• Potassium channel blocking actions of beta-bungarotoxin and related toxins on mouse and frog motor nerve terminals.

  1988cites this paper
• Electrophysiological Characteristics of Peptidergic Nerve Terminals Correlated with Secretion

  1988cites this paper
• Optical studies of the secretory event at vertebrate nerve terminals.

  1988cites this paper
• Regional gradient of pacemaker activity in the early embryonic chick heart monitored by multisite optical recording.

  1988cites this paper
• Optical imaging of neuronal activity.

  1988cites this paper
• Functional pacemaking area in the early embryonic chick heart assessed by simultaneous multiple-site optical recording of spontaneous action potentials

  1988cites this paper
• Optical recording of electrical activity from parallel fibres and other cell types in skate cerebellar slices in vitro.

  1987cites this paper
• Depolarization-induced Ca2+ increase in isolated neurosecretory nerve terminals measured with fura-2.

  1987cites this paper
• Calcium conductance in an identified cholinergic synaptic terminal in the central nervous system of the cockroach

  1987cites this paper
• Visualization of secretory activities in the Xenopus neurohypophysis by a high S/N video camera.

  1987cites this paper
• MSORTV imaging of electrotonic conduction in a syncitium: optical recording of polarization spread in a simple salivary gland.

  1987cites this paper
• Comparison of electrical responses of terminals, axons, and somata of a peptidergic neurosecretory system

  1987cites this paper
• Mapping of early development of electrical activity in the embryonic chick heart using multiple‐site optical recording.

  1987cites this paper
• Optical Studies on Ionic Channels in Intact Vertebrate Nerve Terminals

  1986cites this paper
• Ionic Properties of Burst Generation in Hippocampal Pyramidal Cell Somata ‘In Vitro’

  1986cites this paper
• Action potentials and frequency-dependent secretion in the mouse neurohypophysis.

  1986cites this paper
• Acetylcholine modulates two types of presynaptic potassium channels in vertebrate motor nerve terminals.

  1986cites this paper
• Large and rapid changes in light scattering accompany secretion by nerve terminals in the mammalian neurohypophysis

  1985cites this paper
• Micromolar 4-aminopyridine Enhances Optical Recording of Action Potential Propagation Using an Ultrafast Photodiode-mosfet Camera and a Photodiode Array

  year unknowncites this paper