A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay

Yong Kwan Kim,Seong-In Lim,I. Cho,K. Cheong,Eun-Jeong Lee,Sang-Oh Lee,J. Kim,Jung-Hwa Kim,Dong-Soo Jeong,Byung-Hyun An,D. An

Published 2015 in Journal of Virological Methods

ABSTRACT

Highlights • A novel lateral immunochromatography (IC) assay was developed to detect PEDV antigen.• The IC assay has 96.0% sensitivity and 98.5% specificity compared with real-time reverse transcriptase PCR.• The detection limit for PEDV was 1 × 103 copies.• The IC assay could be stored at 4 °C or room temperature for 15 months without affecting its efficacy.

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