Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel

Cellulosome is a kind of multienzyme complex that displays high activity, selectivity and stability. Here, we report a novel, non-cellulolytic, cellulosome-like multienzyme complex that produced by the Cellulosimicrobium cellulans wild-type strain F16 isolated from soil microflora. This multienzyme complex, with excellent catalytic efficiency of kcat 13.2 s−1 to remove the C-7 xylosyl group from 7-xylosyl-10-deacetylpaclitaxel (10-DAXP), has an outstanding tolerance against organic solvents and an excellent general stability, with the long half-life of 214 hours. This cellulosome-like multienzyme complex has a novel structure distinct from the well-documented ones. The key catalytic subunit responsible for the β-xylosidase activity against 10-DAXP is identified to be a novel protein, indicating a new glycoside hydrolase (GH) family. The pioneering work described here offers a novel nanoscale biocatalyst for the production of biofuels and chemicals from renewable plant-based natural resources.

• Publication year

  2015

• Venue

  Scientific Reports

• Publication date

  2015-09-08

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/srep13768PMID 26347949PMCID 4562250
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Industrial Catalysis A Practical Approach

  2016cited by this paper
• Enzymatic hydrolysis of 7-xylosyltaxanes by an extracellular xylosidase from Cellulosimicrobium cellulans

  2015cited by this paper
• Ultrastable cellulosome-adhesion complex tightens under load

  2014cited by this paper
• Two natural glucomannan polymers, from Konjac and Bletilla, as bioactive materials for pharmaceutical applications

  2014cited by this paper
• PredictProtein—an open resource for online prediction of protein structural and functional features

  2014cited by this paper
• Enzyme immobilisation in biocatalysis: why, what and how.

  2013cited by this paper
• Cloning and Characterization of the Glycoside Hydrolases That Remove Xylosyl Groups from 7-β-xylosyl-10-deacetyltaxol and Its Analogues*

  2013cited by this paper
• Design of protein catalysts.

  2013cited by this paper
• Engineering the third wave of biocatalysis

  2012cited by this paper
• Screening and identification of microbial strains that secrete an extracellular C-7 xylosidase of taxanes

  2011cited by this paper
• Microbial hydrolysis of 7-xylosyl-10-deacetyltaxol to 10-deacetyltaxol

  2011cited by this paper
• The Pfam protein families database

  2011cited by this paper
• De novo assembly of human genomes with massively parallel short read sequencing.

  2010cited by this paper
• CAZymes Analysis Toolkit (CAT): web service for searching and analyzing carbohydrate-active enzymes in a newly sequenced organism using CAZy database.

  2010cited by this paper
• Rapid Qualitative and Quantitative Determination of Seven Valuable Taxanes from Various Taxus Species by UFLC‐ESI‐MS and UFLC‐DAD

  2010cited by this paper
• Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.

  2010cited by this paper
• ArMone: a software suite specially designed for processing and analysis of phosphoproteome data.

  2010cited by this paper
• Green chemistry in the pharmaceutical industry

  2010cited by this paper
• Bacterial diversity of Taxus rhizosphere: culture-independent and culture-dependent approaches.

  2008cited by this paper
• From cellulosomes to cellulosomics.

  2008cited by this paper
• The Pfam protein families database

  2007cited by this paper
• Capillary trap column with strong cation-exchange monolith for automated shotgun proteome analysis.

  2007cited by this paper
• In-gel digestion for mass spectrometric characterization of proteins and proteomes

  2006cited by this paper
• Revisiting the Cellulosimicrobium cellulans yeast-lytic β-1,3-glucanases toolbox: A review

  2006cited by this paper
• The curdlan-type exopolysaccharide produced by Cellulomonas flavigena KU forms part of an extracellular glycocalyx involved in cellulose degradation

  2005cited by this paper
• Cellulosomes: plant-cell-wall-degrading enzyme complexes

  2004cited by this paper
• Microtubules as a target for anticancer drugs

  2004cited by this paper
• Bifunctional family 3 glycoside hydrolases from barley with alpha -L-arabinofuranosidase and beta -D-xylosidase activity. Characterization, primary structures, and COOH-terminal processing.

  2003cited by this paper
• Barley β-d-glucan exohydrolases. Substrate specificity and kinetic properties

  1997cited by this paper
• Enzymic hydrolysis of 7‐xylosyltaxanes by xylosidase from Moraxella sp.

  1997cited by this paper
• Structural role of calcium for the organization of the cellulosome of Clostridium thermocellum.

  1996cited by this paper
• Structures and mechanisms of glycosyl hydrolases.

  1995cited by this paper
• Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum

  1991cited by this paper
• Specialized cell surface structures in cellulolytic bacteria

  1987cited by this paper
• Macromolecular Organization of the Cellulolytic Enzyme Complex of Clostridium thermocellum as Revealed by Electron Microscopy

  1987cited by this paper
• Effects of cellulose on growth, enzyme production, and ultrastructure of a Cellulomonas species

  1986cited by this paper
• The cellulolytic enzyme complex of Clostridium thermocellum is very large.

  1985cited by this paper
• A simple and ultrasensitive method for determination of free fatty acid by radiochemical assay.

  1969cited by this paper
• Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

  1959cited by this paper

• Engineering of 10-Deacetylbaccatin III-10-β-O-Acetyltransferase From Taxus Species for Efficient Acetylating Non-Natural Substrates Into Taxol in Nicotiana benthamiana.

  2026cites this paper
• Companilactobacillus crustorum LMG 23699 and Wickerhamomyces anomalus IMDO 010110 form a candidate, stable, mixed-strain starter culture for sourdough production.

  2025cites this paper
• Fusarium proliferatum PSA-3 Produces Xylanase-Aggregate to Degrade Complex Arabinoxylan

  2025cites this paper
• Bioinformatic Approach to Investigate Larvae Gut Microbiota Cellulosimicrobium protaetiae via Whole-Genome Analysis.

  2024cites this paper
• Screening and characterization of a β-xylosidase from Bifidobacterium breve K-110 and its application in the biotransformation of the total flavonoids of epimedium to icariin with α-l-rhamnosidase.

  2023cites this paper
• Highly Efficient Biotransformation of Notoginsenoside R1 into Ginsenoside Rg1 by Dictyoglomus thermophilum β-xylosidase Xln-DT

  2022cites this paper
• Drug metabolism and pharmacokinetic diversity of Taxus medicinal compounds

  2021cites this paper
• Self-Organization and Information Processing: From Basic Enzymatic Activities to Complex Adaptive Cellular Behavior

  2021cites this paper
• Mining pharmacotherapy utility from chemodiversity/biodiversity of Taxaceae- and Cephalotaxaceae-associated microbes: Molecular mechanisms and functions

  2021cites this paper
• Isolation, Substrate Specificity, and Subunit Characterization of the Xylanosomes Produced by Oerskovia turbata JCM 3160

  2020cites this paper
• Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species.

  2020cites this paper
• Use of Taguchi design for optimization of diesel-oil biodegradation using consortium of Pseudomonas stutzeri, Cellulosimicrobium cellulans, Acinetobacter baumannii and Pseudomonas balearica isolated from tarball in Terengganu Beach, Malaysia

  2020cites this paper
• Cloning and characterization of the β-xylosidase from Dictyoglomus turgidum for high efficient biotransformation of 10-deacetyl-7-xylosltaxol.

  2019cites this paper
• Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species

  2019cites this paper
• Effects of Different Carbon Sources on Enzyme Production and Ultrastructure of Cellulosimicrobium cellulans

  2019cites this paper
• Biotransformation of Glycoginsenosides to Intermediate Products and Aglycones using a Hemicellulosome Produced by Cellulosimicrobium cellulan

  2019cites this paper
• Transcriptome Profile of Polycyclic Aromatic Hydrocarbon-Degrading Fungi Isolated from Taxus rhizosphere

  2019cites this paper
• Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966

  2019cites this paper
• The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

  2018cites this paper
• Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

  2018cites this paper
• Xylanosomes produced by Cellulosimicrobium cellulans F16 were diverse in size, but resembled in subunit composition

  2018cites this paper
• Species of family Promicromonosporaceae and family Cellulomonadeceae that produce cellulosome-like multiprotein complexes

  2018cites this paper
• Metagenomic insights into lignocellulose-degrading genes through Illumina-based de novo sequencing of the microbiome in Vietnamese native goats' rumen.

  2018cites this paper
• Rhizosphere Microbiota and Microbiome of Medicinal Plants: From Molecular Biology to Omics Approaches

  2017cites this paper
• Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production

  2017cites this paper
• Characterization of a recombinant multifunctional glycoside hydrolase family 3 β-xylosidase/α-l-arabinofuranosidase/β-glucosidase from Cellulosimicrobium cellulans sp. 21

  2016cites this paper
• Unearthing microbial diversity of Taxus rhizosphere via MiSeq high-throughput amplicon sequencing and isolate characterization

  2016cites this paper