DNA sequence comparisons of Ophiostoma spp., including Ophiostoma aurorae sp. nov., associated with pine bark beetles in South Africa

Bark beetles (Coleoptera: Scolytinae) are well-recognized vectors of Ophiostoma species. Three non-native bark beetle species infest various Pinus species in South Africa, and they are known to carry at least 12 different species of ophiostomatoid fungi. Some of these fungi have not been identified to species level. The aim of this study was to determine or confirm the identities of Ophiostoma species associated with bark beetles in South Africa using comparisons of DNA sequence data. Identities of Ophiostoma ips, O. floccosum, O. pluriannulatum, O. quercus and O. stenoceras were confirmed. Ophiostoma abietinum, O. piliferum and Pesotum fragrans are recognised for the first time and the new species, O. aurorae sp. nov., is described from pine-infesting bark beetles in South Africa.

• Publication year

  2006

• Venue

  Studies in Mycology

• Publication date

  2006-05-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3114/SIM.55.1.269PMID 18490985PMCID 2104716
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Ophiostoma aurorae sp. nov. is described as a new species from pine-infesting bark beetles in South Africa.

  Confidence 0.99

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• Ophiostoma abietinum, Ophiostoma piliferum, and Pesotum fragrans are recorded from the South African bark beetle-associated fungi for the first time.

  Confidence 0.96

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• DNA sequence comparisons confirmed the identities of Ophiostoma ips, Ophiostoma floccosum, Ophiostoma pluriannulatum, Ophiostoma quercus, and Ophiostoma stenoceras in South African bark beetle-associated material.

  Confidence 0.98

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review

• dna sequence comparisons

  method

  A molecular identification approach that compares fungal DNA sequences to determine or confirm species identities.

  Aliases: sequence comparison, DNA sequence data comparison

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma abietinum

  fungal species

  An Ophiostoma species identified among the bark beetle-associated fungi reported from South Africa.

  Aliases: O. abietinum

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma aurorae sp. nov.

  fungal species, new species

  A newly described Ophiostoma species from pine-infesting bark beetles in South Africa.

  Aliases: Ophiostoma aurorae, O. aurorae sp. nov.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma floccosum

  fungal species

  An Ophiostoma species included among the bark beetle-associated fungi examined in this paper.

  Aliases: O. floccosum

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma ips

  fungal species

  An Ophiostoma species included among the bark beetle-associated fungi examined in this paper.

  Aliases: O. ips

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma piliferum

  fungal species

  An Ophiostoma species identified among the bark beetle-associated fungi reported from South Africa.

  Aliases: O. piliferum

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma pluriannulatum

  fungal species

  An Ophiostoma species included among the bark beetle-associated fungi examined in this paper.

  Aliases: O. pluriannulatum

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma quercus

  fungal species

  An Ophiostoma species included among the bark beetle-associated fungi examined in this paper.

  Aliases: O. quercus

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• ophiostoma stenoceras

  fungal species

  An Ophiostoma species included among the bark beetle-associated fungi examined in this paper.

  Aliases: O. stenoceras

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• pesotum fragrans

  fungal taxon

  A fungal taxon identified among the bark beetle-associated isolates reported from South Africa.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• pine-infesting bark beetles

  insect groups

  Non-native bark beetles that infest Pinus species and act as hosts for the associated ophiostomatoid fungi.

  Aliases: pine bark beetles

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• south africa

  location

  The geographic location where the bark beetle-associated Ophiostoma isolates in this paper were collected.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review

• Fungal Associates of European Bark Beetles With Special Emphasis on the Ophiostomatoid Fungi

  2007cited by this paper
• Phylogeny of the Quambalariaceae fam. nov., including important Eucalyptus pathogens in South Africa and Australia

  2006cited by this paper
• Multi-gene phylogenies define Ceratocystiopsis and Grosmannia distinct from Ophiostoma

  2006cited by this paper
• Ophiostoma dentifundum sp. nov. from oak in Europe, characterized using molecular phylogenetic data and morphology.

  2005cited by this paper
• Taxonomic re-evaluation of Leptographium lundbergii based on DNA sequence comparisons and morphology.

  2005cited by this paper
• Ecology and evolution of mycophagous bark beetles and their fungal partners.

  2005cited by this paper
• Epitypification of Ophiostoma galeiforme and phylogeny of species in the O. galeiforme complex

  2004cited by this paper
• PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)]

  2004cited by this paper
• Characterisation of Ophiostoma species associated with pine bark beetles from Mexico, including O. pulvinisporum sp. nov.

  2004cited by this paper
• Two new Ophiostoma species with Sporothrix anamorphs from Austria and Azerbaijan.

  2004cited by this paper
• Ceratocystis species infecting stem wounds on Eucalyptus grandis in South Africa

  2004cited by this paper
• Leptographium wingfieldii introduced into North America and found associated with exotic Tomicus piniperda and native bark beetles.

  2004cited by this paper
• Diversity of saprobic hyphomycetes on Proteaceae and Restionaceae from South Africa

  2004cited by this paper
• Identity and phylogenetic relationships of ophiostomatoid fungi associated with invasive and native Tetropium species (Coleoptera: Cerambycidae) in Atlantic Canada.

  2003cited by this paper
• Phylogeny of the Ophiostoma stenoceras–Sporothrix schenckii complex

  2003cited by this paper
• The Ophiostoma piceae complex in the Southern Hemisphere: a phylogenetic study.

  2003cited by this paper
• Bark Beetle?fungus Symbioses

  2003cited by this paper
• Ceratocystis pirilliformis, a new species from Eucalyptus nitens in Australia.

  2003cited by this paper
• MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform.

  2002cited by this paper
• PAUP*: Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10

  2002cited by this paper
• Ophiostomatoid fungi associated with three pine-infesting bark beetles in South Africa.

  2001influential reference
• Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi

  2001cited by this paper
• Ophiostoma africanum sp. nov., and a key to ophiostomatoid species from Protea infructescences

  2001cited by this paper
• Leptographium Species: Tree Pathogens, Insect Associates, and Agents of Blue-Stain

  2001cited by this paper
• Sporotrichosis Infection on Mines of the Witwatersrand

  2000cited by this paper
• On the phylogeny of members of Ceratocystis s.s. and Ophiostoma that possess different anamorphic states, with emphasis on the anamorph genus Leptographium, based on partial ribosomal DNA sequences

  2000cited by this paper
• First Report of Rhizosis Caused by Ceratocystis radicicola on Date Palms in South Africa.

  1999cited by this paper
• A molecular phylogenetic reappraisal of the graphium complex based on 18S rDNA sequences

  1998cited by this paper
• Ophiostoma protearum sp.nov. associated with Protea caffra infructescences

  1997cited by this paper
• A new Ceratocystis species defined using morphological and ribosomal DNA sequence comparisons

  1996cited by this paper
• Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes

  1995cited by this paper
• Graphium pseudormiticum sp. nov.: a new hyphomycete with unusual conidiogenesis

  1994cited by this paper
• The polyphyletic origins of ophiostomatoid fungi

  1994cited by this paper
• Fungi associated with infructescences of Protea species in South Africa, including a new species of ophiostoma

  1994cited by this paper
• Ceratocystis and Ophiostoma: Taxonomy, Ecology, and Pathogenicity

  1993cited by this paper
• Cartapip™: a biopulping product for control of pitch and resin acid problems in pulp mills

  1993cited by this paper
• A new species of Ophiostoma from Protea infructescences in South Africa

  1993cited by this paper
• Sporothrix eucalypti (sp. nov.), a shoot and leaf pathogen ofEucalyptus in South Africa

  1993cited by this paper
• ITS primers with enhanced specificity for basidiomycetes ‐ application to the identification of mycorrhizae and rusts

  1993cited by this paper
• A Catalog of Scolytidae and Platypodidae (Coleoptera), Part 2: Taxonomic Index. Volume A

  1992cited by this paper
• Colonisation sites on Pinus radiata logs of the bark beetles, Orthotomicus erosus, Hylastes angustatus and Hylurgus ligniperda (Coleoptera: Scolytidae).

  1992cited by this paper
• Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

  1990cited by this paper
• New conifer-inhabiting species of Ophiostoma and Ceratocystiopsis (Ascomycetes, Microascales) from Mexico.

  1990cited by this paper
• Leptographium root diseases on conifers

  1989cited by this paper
• Ceratocystiopsis proteae sp. nov., with a new anamorph genus

  1988cited by this paper
• The neighbor-joining method: a new method for reconstructing phylogenetic trees.

  1987cited by this paper
• CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP

  1985cited by this paper
• Some Verticicladiella species, including V. truncata sp. nov., associated with root diseases of pine in New Zealand and South Africa.

  1983cited by this paper
• Verticicladiella alacris sp. nov., associated with a root disease of pines in South Africa.

  1980cited by this paper
• Ceratocystis ips associated with Orthotomicus erosus (Coleoptera: Scolytidae) on Pinus spp. in the Cape Province of South Africa.

  1980cited by this paper
• Leptographium reconditum sp.nov. and observations on conidiogenesis in Verticicladiella

  1978cited by this paper
• Index of plant pathogens and the diseases they cause in cultivated plants in South Africa.

  1977cited by this paper
• The mycoflora of an alkaline soil of the open-savannah of the transvaal

  1974cited by this paper
• Three new Raffaelea species

  1970influential reference
• New and Interesting Records of South African Fungi, Part VI

  1966cited by this paper
• New and Interesting Records of South African Fungi. Part II.

  1956cited by this paper
• Eine Übersicht über die gewöhnlichsten mit Borkenkäfern assoziierten Bläuepilze in Schweden und einige für Schweden neue Bläuepilze

  1953cited by this paper
• Growth, Yield and Nutrition in Carpets of a Forest Moss (Hylocomium splendens)

  1952cited by this paper
• New and Interesting Records of South African Fungi.

  1951cited by this paper
• The South African fungi and lichens to the end of 1945.

  1950cited by this paper
• The South African fungi and lichens.

  1950cited by this paper
• Permanent stained mycological preparations obtained by slide culture.

  1950cited by this paper

• Ophiostoma babimostense and Sporothrix europaea (Ascomycota, Ophiostomatales), two new ophiostomatalean species, associated with ambrosia and bark beetles in Norway and Poland

  2025cites this paper
• Ophiostomatoid fungi associated with Hylurgus ligniperda, including six new species from eastern China

  2025cites this paper
• Insights from Cutting-edge Diagnostics and Epidemiology of Sporotrichosis and Taxonomic Shifts in Sporothrix

  2025cites this paper
• Association between sub-cortical beetles, mites, and ophiostomatoid fungi on trees in South African Afromontane forests

  2024cites this paper
• Fungal Communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales With Bursaphelenchus xylophilus

  2022cites this paper
• Generic boundaries in the Ophiostomatales reconsidered and revised

  2022cites this paper
• Ceratocystiopsis quercina sp. nov. Associated with Platypus cylindrus on Declining Quercus suber in Portugal

  2022cites this paper
• Ophiostomatoid fungi associated with pine bark beetles and infested pines in south-eastern Australia, including Graphilbum ipis-grandicollis sp. nov.

  2021cites this paper
• Six new species of Sporothrix from hardwood trees in Poland

  2021cites this paper
• Investigating the effects of nitrogen deposition and substrates on the microbiome and mycobiome of the millipede Cherokia georgiana georgiana (Diplopoda: Polydesmida)

  2021cites this paper
• New ophiostomatoid fungi from wounds on storm-damaged trees in Afromontane forests of the Cape Floristic Region

  2020cites this paper
• The threat of emerging and re-emerging pathogenic Sporothrix species

  2020cites this paper
• Sporotrichosis: The Emerging Fungal Threats to Animals

  2019cites this paper
• Productivity and mortality of Acacia mangium willd. plantation with associated bark beetle and Nitidulid beetle in east Kalimantan, Borneo, Indonesia

  2019influential citation
• Ophiostomatoid fungi associated with pines infected by Bursaphelenchusxylophilus and Monochamusalternatus in China, including three new species

  2018cites this paper
• Isolation and identification of Sydowia polyspora and its pathogenicity on Pinus yunnanensis in Southwestern China

  2018cites this paper
• Identification of sapstain fungi from Scots pine pallets and assessment of their staining ability

  2018cites this paper
• Diversity of Ophiostomatales species associated with conifer-infesting beetles in the Western Carpathians

  2017cites this paper
• Fungi and insects associated with Euphorbia ingens die-off in South Africa

  2017cites this paper
• Ophiostomatoid fungi associated with conifer-infesting beetles and their phoretic mites in Yunnan, China

  2017cites this paper
• Two new species of Xanthagaricus and some notes on Heinemannomyces from Asia

  2017cites this paper
• Ophiostomatoid fungi associated with bark beetles infesting broadleaved trees in Norway

  2017cites this paper
• Fungal communities associated with bark and ambrosia beetles trapped at international harbours

  2017cites this paper
• Ophiostoma sugadairense, a new species in the Ophiostoma piceae complex associated with bark beetles infesting Japanese larch in Japan

  2017cites this paper
• Three new species of Ophiostomatales from Nothofagus in Patagonia

  2016cites this paper
• Wounds on Rapanea melanophloeos provide habitat for a large diversity of Ophiostomatales including four new species

  2016cites this paper
• Multigene phylogenies and morphological characterization of five new Ophiostoma spp. associated with spruce-infesting bark beetles in China.

  2016cites this paper
• Ophiostomatoid fungi associated with mangroves in South Africa, including Ophiostoma palustre sp. nov.

  2016cites this paper
• Sporothrix chilensis sp. nov. (Ascomycota: Ophiostomatales), a soil-borne agent of human sporotrichosis with mild-pathogenic potential to mammals.

  2016cites this paper
• The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

  2016influential citation
• Symbiotic Associations of Bark Beetles

  2015cites this paper
• New species of Ophiostomatales from Scolytinae and Platypodinae beetles in the Cape Floristic Region, including the discovery of the sexual state of Raffaelea

  2015cites this paper
• Draft genome sequences of Ceratocystis eucalypticola , Chrysoporthe cubensis , C . deuterocubensis , Davidsoniella virescens , Fusarium temperatum , Graphilbum fragrans , Penicillium nordicum , and Thielaviopsis musarum

  2015cites this paper
• Molecular Diagnosis of Pathogenic Sporothrix Species

  2015cites this paper
• IMA Genome-F 5

  2015cites this paper
• Biodiversity and ecology of ophiostomatoid fungi associated with trees in the Cape floristic region of South Africa

  2014influential citation
• Multigene phylogenies of Ophiostomataceae associated with Monterey pine bark beetles in Spain reveal three new fungal species

  2014cites this paper
• Association of the pine-infesting Pissodes species with ophiostomatoid fungi in Poland

  2013cites this paper
• Les champignons associés au Platypus sylindrus Fab.(Coleoptera Curculionidae, Platypodinae) dans un jeune peuplement de chêne-liège de la forêtde M'Sila(Oran,nord-ouest d'Algerie):Etude particulièrede la biologie et l'épidémiologie de l'insecte

  2013cites this paper
• Emergence of pathogenicity in the Sporothrix schenckii complex.

  2013cites this paper
• Phylogenetic Analysis Reveals a High Prevalence of Sporothrix brasiliensis in Feline Sporotrichosis Outbreaks

  2013cites this paper
• Diversity of ophiostomatoid fungi associated with the large pine weevil, Hylobius abietis, and infested Scots pine seedlings in Poland

  2013cites this paper
• Global ITS diversity in the Sporothrix schenckii complex

  2013cites this paper
• Large Shift in Symbiont Assemblage in the Invasive Red Turpentine Beetle

  2013cites this paper
• Ophiostomatoid fungi associated with root-feeding bark beetles on Scots pine in Poland

  2013cites this paper
• Ophiostomatoid fungi associated with Ips sexdentatus on Pinus sylvestris in Poland.

  2012cites this paper
• Grosmannia and Leptographium spp. associated with conifer-infesting bark beetles in Finland and Russia, including Leptographium taigense sp. nov.

  2012cites this paper
• Biodiversity in dead wood.

  2012cites this paper
• A diverse assemblage of Ophiostoma species, including two new taxa on eucalypt trees in South Africa

  2012influential citation
• Mycobiota associated with Platypus cylindrus (Coleoptera: Curculionidae, Platypodidae) in cork oak stands of North West Algeria, Africa

  2011cites this paper
• Leptographium tereforme sp. nov. and other Ophiostomatales isolated from the root-feeding bark beetle Hylurgus ligniperda in California

  2011cites this paper
• Molecular and phenotypic characterization of anamorphic fungi

  2011cites this paper
• Fungal associates of the lodgepole pine beetle, Dendroctonus murrayanae

  2011cites this paper
• Characterisation of synnematous bark beetle-associated fungi from China, including Graphium carbonarium sp. nov.

  2010cites this paper
• Ophiostoma species, including Ophiostoma borealis sp. nov., infecting wounds of native broad-leaved trees in Norway

  2010cites this paper
• Ophiostoma tsotsi sp. nov., A Wound-infesting Fungus of Hardwood Trees in Africa

  2010cites this paper
• Sporothrix brunneoviolacea and Sporothrix dimorphospora, two new members of the Ophiostoma stenoceras-Sporothrix schenckii complex

  2010cites this paper
• A new Ophiostoma species from loblolly pine roots in the southeastern United States

  2010influential citation
• Ophiostoma spp. associated with pine- and spruce-infesting bark beetles in Finland and Russia

  2010cites this paper
• Delimitation of Ophiostoma quercus and its synonyms using multiple gene phylogenies

  2009influential citation
• Antifungal activity of neutralized wood vinegar with water extracts of Pinus densiflora and Quercus serrata saw dusts.

  2009cites this paper
• Identification and application of mating type gene sequences in Ophiostoma

  2009cites this paper
• Ophiostoma denticiliatum sp. nov. and other Ophiostoma species associated with the birch bark beetle in southern Norway

  2009cites this paper
• Ophiostoma gemellus and Sporothrix variecibatus from mites infesting Protea infructescences in South Africa

  2008cites this paper
• Taxonomy and phylogeny of new wood- and soil-inhabiting Sporothrix species in the Ophiostoma stenoceras-Sporothrix schenckii complex

  2008cites this paper
• Phylogenetic classification of Cordyceps and the clavicipitaceous fungi

  2007cites this paper
• EDITORIAL AND REFLECTION

  2007cites this paper
• Impact of DNA sequence-data on the taxonomy of anamorphic fungi

  2007cites this paper
• Ectinogonia buquetti Spinola (COLEOPTERA) VECTOR DE Ophiostoma stenoceras (Robak) Melin & Nannf. EN BOSQUES DE Eucalyptus DE LA V REGION

  2006cites this paper
• How many species of fungi are there at the tip of Africa?

  2006cites this paper