Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh)

The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L−1 FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.

• Publication year

  2015

• Venue

  Brazilian Journal of Microbiology

• Publication date

  2015-06-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1590/S1517-838246220140208PMID 26273257PMCID 4507534
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Degradation of Clodinafop Propargyl by Pseudomonas sp. Strain B2

  2013influential reference
• Biodegradation of butachlor by Rhodococcus sp. strain B1 and purification of its hydrolase (ChlH) responsible for N-dealkylation of chloroacetamide herbicides.

  2012cited by this paper
• MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.

  2011influential reference
• Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh.

  2011influential reference
• Degradation of cyhalofop-butyl (CyB) by Pseudomonas azotoformans strain QDZ-1 and cloning of a novel gene encoding CyB-hydrolyzing esterase.

  2011influential reference
• Cloning of a Novel Pyrethroid-Hydrolyzing Carboxylesterase Gene from Sphingobium sp. Strain JZ-1 and Characterization of the Gene Product

  2009cited by this paper
• More toxic and photoresistant products from photodegradation of fenoxaprop-p-ethyl.

  2008cited by this paper
• Evolution of toxicity upon hydrolysis of fenoxaprop-p-ethyl.

  2007cited by this paper
• Separation of Fenoxaprop-p-ethyl Biodegradation Products by HPTLC

  2005influential reference
• Biodegradation of fenoxaprop-p-ethyl by bacteria isolated from sludge.

  2005influential reference
• Performance of Clodinafop and Fenoxaprop-p-ethyl for the Control of Phalaris minor in Wheat

  1998cited by this paper
• Biotransformations of Fenoxaprop-ethyl by Fluorescent Pseudomonas Strains

  1998influential reference
• The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.

  1997influential reference
• Degradation of diclofop-methyl by pure cultures of bacteria isolated from Manitoban soils.

  1996influential reference
• Isolation and characterization of soil microorganisms capable of utilizing the herbicide diclofop-methyl as a sole source of carbon and energy.

  1996influential reference
• Behaviour of fenoxaprop-ethyl in the environment - degradation and effect in the ecosystem.

  1990cited by this paper
• Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains

  1989cited by this paper
• International Committee on Systematic Bacteriology: announcement of the report of the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics.

  1988influential reference
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Bergey's Manual of Determinative Bacteriology.

  1975cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• [109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA

  1968cited by this paper
• Bergey's Manual of Determinative Bacteriology

  1948cited by this paper

• The Novel Enantioselective Esterase AopE from Brevundimonas sp. Strain YS Preferentially Catabolizes the (R)-Enantiomer of the Herbicide Haloxyfop-Methyl.

  2025cites this paper
• Plant growth-promoting rhizobacteria in pesticide biodegradation: a comprehensive review

  2025cites this paper
• QpmH esterase from cotton rhizosphere bacteria: A novel approach for degrading quizalofop-p-ethyl herbicide.

  2025cites this paper
• Efficient Biodegradation of Multiple Aryloxyphenoxypropionate Herbicides by Corynebacterium sp. Z-1 and the Proposed Degradation Mechanism.

  2024cites this paper
• Characterization of two novel hydrolases from Sphingopyxis sp. DBS4 for enantioselective degradation of chiral herbicide diclofop-methyl.

  2024cites this paper
• Mechanistic Insight into the Enantioselective Degradation of Esterase QeH to (R)/(S)–Quizalofop–Ethyl with Molecular Dynamics Simulation Using a Residue-Specific Force Field

  2024cites this paper
• Cloning of cyp57A1 gene from Fusarium verticillioides for degradation of herbicide fomesafen

  2024cites this paper
• A new multiresidue thin layer chromatography–ultraviolet detection method for the rapid determination of six herbicides residue applied as ready mix formulation for rice

  2023cites this paper
• Kinetic and mechanism investigation on the gamma irradiation induced degradation of quizalofop-p-ethyl.

  2021cites this paper
• Atlas of the microbial degradation of fluorinated pesticides

  2021cites this paper
• Potential Role of Endophytes in Weeds and Herbicide Tolerance in Plants

  2021cites this paper
• Kinetic and mechanism investigation on the gamma irradiation induced degradation of quizalofop-p-ethyl

  2021cites this paper
• Niastella caeni sp. nov., isolated from activated sludge.

  2020cites this paper
• Impact of biosolids, ZnO, ZnO/biosolids on bacterial community and enantioselective transformation of racemic-quizalofop-ethyl in agricultural soil.

  2020cites this paper
• Screening and degradation characteristics of a tylosin-degrading strain

  2020cites this paper
• Biochemical and molecular biological studies on enzymatic synthesis of vitamin B6 derivatives and optically active carboxylic acids

  2020cites this paper
• Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase

  2020cites this paper
• The Genome Analysis of Methylobacterium populi YC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase

  2020cites this paper
• Bioremediation of Contaminated Environments Using Rhodococcus

  2019cites this paper
• Isolation , identification and characterization of clodinafop-propargyl degrading Sphingopyxis sp . WP 68

  2019influential citation
• A novel method of producing the pharmaceutical intermediate (R)-2-chloromandelic acid by bioconversion

  2019cites this paper
• Nanometer Titanium Dioxide Mediated High Efficiency Photodegradation of Fluazifop-p-Butyl

  2019cites this paper
• Characterization of a novel aryloxyphenoxypropionate herbicide-hydrolyzing carboxylesterase with R-enantiomer preference from Brevundimonas sp. QPT-2

  2019cites this paper
• Technologies for the Selection, Culture and Metabolic Profiling of Unique Rhizosphere Microorganisms for Natural Product Discovery

  2019cites this paper
• Recent insights into the microbial catabolism of aryloxyphenoxy-propionate herbicides: microbial resources, metabolic pathways and catabolic enzymes

  2018cites this paper
• Pesticide Pollution in Agricultural Soils and Sustainable Remediation Methods: a Review

  2018cites this paper
• Microbial Degradation of Chemical Pesticides and Bioremediation of Pesticide-Contaminated Sites in China

  2018cites this paper
• Purification and properties of a novel quizalofop-p-ethyl-hydrolyzing esterase involved in quizalofop-p-ethyl degradation by Pseudomonas sp. J-2

  2017influential citation
• A key esterase required for the mineralization of quizalofop-p-ethyl by a natural consortium of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9.

  2017cites this paper
• Identification and characterization of a novel carboxylesterase (FpbH) that hydrolyzes aryloxyphenoxypropionate herbicides

  2017cites this paper
• Microbial degradation of pesticide: A review

  2017cites this paper
• Cytotoxicity and genotoxicity of fenoxaprop-p-ethyl and fluzifob-p-butyl herbicides

  2017cites this paper
• Biodegradation of CladinafopPropargyl by Bacillus sp. and Bacillus cereus Isolated from Field Crop

  2017cites this paper
• Modelling aerobic biodegradation of atrazine and 2,4-dichlorophenoxy acetic acid by mixed-cultures.

  2017cites this paper
• Characterization of EstQE, a new member of esterase family VIII from the quizalofop-P-ethyl-degrading bacterium Ochrobactrum sp. QE-9

  2016cites this paper