Biodegradation of di-n-Butyl Phthalate by Achromobacter sp. Isolated from Rural Domestic Wastewater

A bacterial strain W-1, isolated from rural domestic wastewater, can utilize the environmental hormone di-n-butyl phthalate (DBP) as the sole carbon and energy source. The isolated bacterium species was confirmed to belong to the genus Achromobacter based on its 16S rRNA gene sequence. The results of substrate utilization tests showed that the strain W-1 could utilize other common phthalates and phenol. High-performance liquid chromatography analysis revealed that the optimal conditions for DBP degradation were pH 7.0, 35 °C, and an agitation rate of 175 rpm. Under these conditions, 500 mg/L of DBP was completely degraded within 30 h. The effects of heavy metals (50 mg/L Cu2+ and 500 mg/L Pb2+) and surfactants (100 mg/L SDS and 500 mg/L Tween 20) on DBP degradation were investigated. The results demonstrated that Cu2+ and SDS severely inhibited DBP degradation and Pb2+ weakly inhibited DBP degradation, while Tween 20 greatly enhanced DBP degradation. Furthermore, phthalate degradation genes were found to be located on a plasmid present in Achromobacter sp. W-1.

• Publication year

  2015

• Venue

  International Journal of Environmental Research and Public Health

• Publication date

  2015-10-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/ijerph121013510PMID 26516878PMCID 4627046
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Achromobacter denitrificans strain SP1 efficiently remediates di(2-ethylhexyl)phthalate.

  2015influential reference
• Occurrence and risk assessment of phthalate esters (PAEs) in vegetables and soils of suburban plastic film greenhouses.

  2015cited by this paper
• Effects of Potamogeton crispus L.-bacteria interactions on the removal of phthalate acid esters from surface water.

  2015cited by this paper
• Toxicity and Estrogenic Endocrine Disrupting Activity of Phthalates and Their Mixtures

  2014cited by this paper
• Effects of combined exposure to 17α-ethynylestradiol and dibutyl phthalate on the growth and reproduction of adult male zebrafish (Danio rerio).

  2014cited by this paper
• Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation

  2014cited by this paper
• Biodegradation of acetochlor by a newly isolated Achromobacter sp. strain D-12

  2013cited by this paper
• Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.

  2013cited by this paper
• Cloning of a dibutyl phthalate hydrolase gene from Acinetobacter sp. strain M673 and functional analysis of its expression product in Escherichia coli

  2013cited by this paper
• Biodegradation of di-n-butyl phthalate by an isolated Gordonia sp. strain QH-11: Genetic identification and degradation kinetics.

  2012cited by this paper
• Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate by newly isolated Agrobacterium sp. and the biochemical pathway

  2011cited by this paper
• Biodegradation of di-n-butyl phthalate by Rhodococcus sp. JDC-11 and molecular detection of 3, 4-phthalate dioxygenase gene.

  2010cited by this paper
• Complete Genome Sequence of the Haloaromatic Acid-Degrading Bacterium Achromobacter xylosoxidans A8

  2010cited by this paper
• Dibutyl phthalate degradation by Enterobacter sp. T5 isolated from municipal solid waste in landfill bioreactor.

  2010cited by this paper
• Bioremediation of endocrine disruptor di-n-butyl phthalate ester by Deinococcus radiodurans and Pseudomonas stutzeri.

  2010cited by this paper
• Bacterial degradation of phthalate isomers and their esters

  2008cited by this paper
• Degradation of p-nitrophenol by Achromobacter xylosoxidans Ns isolated from wetland sediment

  2007cited by this paper
• Chromate reduction by resting cells of Achromobacter sp. Ch-1 under aerobic conditions

  2007cited by this paper
• A Mono-2-Ethylhexyl Phthalate Hydrolase from a Gordonia sp. That Is Able To Dissimilate Di-2-Ethylhexyl Phthalate

  2006cited by this paper
• Subchronic exposure to low concentrations of di-n-butyl phthalate disrupts spermatogenesis in Xenopus laevis frogs.

  2005cited by this paper
• Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate ester by Pseudomonas fluorescens B-1

  2005cited by this paper
• Biodegradation of an endocrine-disrupting chemical, di-2-ethylhexyl phthalate, by Bacillus subtilis No. 66

  2005cited by this paper
• Biodegradation of 2,4-dichlorophenol and phenol in an airlift inner-loop bioreactor immobilized with Achromobacter sp

  2004cited by this paper
• Phthalate catabolic gene cluster is linked to the angular dioxygenase gene in Terrabacter sp. strain DBF63

  2003cited by this paper
• Occurrence and microbial degradation of phthalate esters in Taiwan river sediments.

  2002cited by this paper
• Plasmid-Encoded Phthalate Catabolic Pathway in Arthrobacter keyseri 12B

  2001cited by this paper
• Isolation and curing of the Klebsiella pneumoniae large indigenous plasmid using sodium dodecyl sulphate.

  2000cited by this paper
• Analysis of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pEST4011 of Achromobacter xylosoxidans subsp. denitrificans strain EST4002.

  2000cited by this paper
• Plasmid-mediated degradation of o- and p-phthalate by Pseudomonas fluorescens

  1999cited by this paper
• Novel Organization of the Genes for Phthalate Degradation from Burkholderia cepacia DBO1

  1998cited by this paper
• The environmental fate of phthalate esters: A literature review

  1997cited by this paper
• Genes in PHT plasmid encoding the initial degradation pathway of phthalate in Pseudomonas putida

  1992cited by this paper

• Di-butyl phthalates (DBP) in the environment: health risks and advances in treatment technologies

  2025cites this paper
• Alicycliphilus soli sp. nov., Isolated from a Plastic Film Greenhouse Soil and the Proposal for the Reclassification of Diaphorobacter limosus as Alicycliphilus limosus comb. nov.

  2025cites this paper
• DAPK3 is Essential for DBP‐Induced Autophagy of Mouse Leydig Cells

  2025cites this paper
• The Efficient PAE-Degrading Performance and Complete Genome Sequencing of Gordonia sp. LUNF6

  2025cites this paper
• Enhanced catalytic performance of Rhizomucor miehei lipase on di-n-butyl and diethylhexyl phthalates: insights into substrate specificity and immobilization strategy

  2025cites this paper
• Biodegradation of plasticizers by novel strains of bacteria isolated from plastic waste near Juhu Beach, Mumbai, India

  2024cites this paper
• Distribution characteristics and relationship of microplastics, phthalate esters, and bisphenol A in the Beiyun River basin of Beijing.

  2024cites this paper
• Whole-cell biocatalysis for phthalate esters biodegradation in wastewater by a salt tolerant bacterial SSB-consortium.

  2024cites this paper
• Simultaneous organic wastewater treatment and bioelectricity production in a dual chamber microbial fuel cell with Scenedesmus obliquus biocathode

  2024cites this paper
• Phthalate monoesters accumulation in residential indoor dust and influence factors.

  2024cites this paper
• Dibutyl phthalate degradation by Paenarthrobacter ureafaciens PB10 through downstream product myristic acid and its bioremediation potential in contaminated soil.

  2024cites this paper
• Biodegradation of diethyl phthalate and phthalic acid by a new indigenous Pseudomonas putida

  2023cites this paper
• Biological detoxification of oxytetracycline using Achromobacter-immobilized bioremediation system

  2023cites this paper
• Biodegradation of di(2-ethylhexyl) phthalate by a new bacterial consortium.

  2023cites this paper
• Degradation of dibutyl phthalate from synthetic and real wastewater using ultrasound/hydrogen peroxide system

  2023cites this paper
• Biodegradation of phthalates and metabolic pathways: an overview

  2023cites this paper
• Tributyltin degrading microbial enzymes: A promising remediation approach.

  2023cites this paper
• Risk Assessment of Phthalate Esters in Baiyangdian Lake and Typical Rivers in China

  2023cites this paper
• Synthesis of Fe3O4@PAC as a magnetic nano-composite for adsorption of dibutyl phthalate from the aqueous medium: Modeling, analysis and optimization using the response surface methodology

  2022cites this paper
• Recent progress in treatment of dyes wastewater using microbial-electro-Fenton technology

  2022cites this paper
• Towards passive bioremediation of dye-bearing effluents using hydrous ferric oxide wastes: Mechanisms, products and microbiology.

  2022cites this paper
• Spatiotemporal Evaluation of Water Quality and Hazardous Substances in Small Coastal Streams According to Watershed Characteristics

  2022cites this paper
• Biodegradation of Terephthalic Acid by Isolated Active Sludge Microorganisms and Monitoring of Bacteria in a Continuous Stirred Tank Reactor

  2021cites this paper
• Spatial distribution, historical trend, and ecological risk assessment of phthalate esters in sediment from Taihu Lake, China

  2021cites this paper
• Phthalate pollution: environmental fate and cumulative human exposure index using the multivariate analysis approach.

  2021cites this paper
• Biodegradation of diisononyl phthalate by a consortium of saline soil bacteria: optimisation and kinetic characterisation

  2021influential citation
• An overview of neonicotinoids: biotransformation and biodegradation by microbiological processes

  2021cites this paper
• Bacterial consortium for efficient degradation of di-ethyl phthalate in soil microcosm

  2021cites this paper
• Degradation of dibutyl phthalate by Paenarthrobacter sp. Shss isolated from Saravan landfill, Hyrcanian Forests, Iran

  2021cites this paper
• Screening And Genome Sequencing of a di-n-butyl Phthalate Degrading Bacterium J2 Isolated From Peanut Field Soil

  2021cites this paper
• Pollution characteristics, spatial variation, and potential risks of phthalate esters in the water-sediment system of the Yangtze River estuary and its adjacent East China Sea.

  2020cites this paper
• Indigenous Bacillus paramycoides and Alcaligenes faecalis: potential solution for the bioremediation of wastewaters

  2020cites this paper
• Biodegradation of recalcitrant compounds and phthalates by a consortium of Liometopum apiculatum gut microbiota

  2020cites this paper
• Bioremediation of Bisphenols and Phthalates from Industrial Effluents: A Review

  2020cites this paper
• Dibutyl phthalate contamination remolded the fungal community in agro-environmental system.

  2019cites this paper
• Bioremediation of dibutyl phthalate in a simulated agricultural ecosystem by Gordonia sp. strain QH-11 and the microbial ecological effects in soil.

  2019cites this paper
• Exposure and ecological risk of phthalate esters in the Taihu Lake basin, China.

  2019cites this paper
• PMBD: a Comprehensive Plastics Microbial Biodegradation Database

  2019cites this paper
• Biodegradation of Selected Endocrine Disrupting Compounds

  2018cites this paper
• Responses of bacterial community to dibutyl phthalate pollution in a soil-vegetable ecosystem.

  2018cites this paper
• Title : Investigation of the Active Biofilm Communities on Polypropylene 1 Filter Media in a Fixed Biofilm Reactor for Wastewater Treatment 2 3 Running Title : Wastewater Treating Biofilms in Polypropylene Media Reactors 4 Contributors : 5

  2018cites this paper
• Investigation of the active biofilm communities on polypropylene filter media in a fixed biofilm reactor for wastewater treatment

  2018cites this paper
• Deciphering the Anode-Enhanced Azo Dye Degradation in Anaerobic Baffled Reactors Integrating With Microbial Fuel Cells

  2018cites this paper
• Characterization and Genome Analysis of a Phthalate Esters-Degrading Strain Sphingobium yanoikuyae SHJ

  2018cites this paper
• Performance of the biological aerated filter bioaugmented by a yeast Magnusiomyces ingens LH-F1 for treatment of Acid Red B and microbial community dynamics

  2017cites this paper
• Performance of the biological aerated filter bioaugmented by a yeast Magnusiomyces ingens LH-F1 for treatment of Acid Red B and microbial community dynamics

  2017cites this paper
• Profiles and risk assessment of phthalate acid esters (PAEs) in drinking water sources and treatment plants, East China

  2017cites this paper
• Differential fluoride tolerance between sulfur- and ferrous iron-grown Acidithiobacillus ferrooxidans and its mechanism analysis

  2017cites this paper
• Bactérias degradadoras de lactose e glúten presentes em queijos e iogurtes encontrados no mercado de Manaus: alternativas para a intolerância à lactose e à Doença Celíaca

  2017cites this paper
• Insights into the fluoride-resistant regulation mechanism of Acidithiobacillus ferrooxidans ATCC 23270 based on whole genome microarrays

  2016cites this paper
• Association between fertilizer-mediated changes in microbial communities and Aedes albopictus growth and survival.

  2016cites this paper
• Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp. YC-RL4 and the bioprocess with environmental samples

  2016cites this paper
• Achromobacter denitrificans SP1 efficiently utilizes 16 phthalate diesters and their downstream products through protocatechuate 3,4-cleavage pathway.

  2016cites this paper