CHD1 and CHD2 are positive regulators of HIV-1 gene expression

BackgroundRetroviruses encode a very limited number of proteins and therefore must exploit a wide variety of host proteins for completion of their lifecycle.MethodsWe performed an insertional mutagenesis screen to identify novel cellular regulators of retroviral replication.ResultsThis approach identified the ATP-dependent chromatin remodeler, chromodomain helicase DNA-binding protein 2 (CHD2), as well as the highly related CHD1 protein, as positive regulators of both MLV and HIV-1 replication in rodent and human cells. RNAi knockdown of either CHD2 or the related CHD1 protein, in human cells resulted in a block to infection by HIV-1, specifically at the level of transcription.ConclusionsThese results demonstrate that CHD1 and CHD2 can act as positive regulators of HIV-1 gene expression.

• Publication year

  2014

• Venue

  Virology Journal

• Publication date

  2014-10-08

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/1743-422X-11-180PMID 25297984PMCID 4283154
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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