Observation of transient disorder during myosin subfragment-1 binding to actin by stopped-flow fluorescence and millisecond time resolution electron cryomicroscopy: evidence that the start of the crossbridge power stroke in muscle has variable geometry.

No abstract is available for this paper.

• Publication year

  1999

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  1999-01-19

• Fields of study

  Biology, Medicine, Physics, Chemistry

• Identifiers
  DOI 10.1073/PNAS.96.2.465PMID 9892656PMCID PMC15159
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The mechanism of muscular contraction.

  2002cited by this paper
• The mechanism of muscular contraction. Science 164:1356-1366, 1969.

  2002cited by this paper
• Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin.

  1998cited by this paper
• A computer-controlled spraying-freezing apparatus for millisecond time-resolution electron cryomicroscopy.

  1998cited by this paper
• Quick-freeze deep-etch electron microscopy of the actin-heavy meromyosin complex during the in vitro motility assay.

  1998cited by this paper
• Kinetics of nucleoside triphosphate cleavage and phosphate release steps by associated rabbit skeletal actomyosin, measured using a novel fluorescent probe for phosphate.

  1997cited by this paper
• Muscle proteins--their actions and interactions.

  1996cited by this paper
• Low pH induces swiveling of the glycoprotein heterodimers in the Semliki Forest virus spike complex.

  1995cited by this paper
• Millisecond time resolution electron cryo-microscopy of the M-ATP transient kinetic state of the acto-myosin ATPase.

  1995cited by this paper
• Muscle cross-bridges bound to actin are disordered in the presence of 2,3-butanedione monoxime.

  1995cited by this paper
• A 35-Å movement of smooth muscle myosin on ADP release

  1995cited by this paper
• Electron cryomicroscopy of acto-myosin-S1 during steady-state ATP hydrolysis.

  1994cited by this paper
• Rotational dynamics of actin-bound intermediates of the myosin adenosine triphosphatase cycle in myofibrils.

  1994cited by this paper
• Analysis of transient structures by cryo-microscopy combined with rapid mixing of spray droplets.

  1994cited by this paper
• Direct visualization by electron microscopy of the weakly bound intermediates in the actomyosin adenosine triphosphatase cycle.

  1993cited by this paper
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  1993cited by this paper
• Time-resolved electron microscopic analysis of the behavior of myosin heads on actin filaments after photolysis of caged ATP

  1993cited by this paper
• The actomyosin ATPase: a two-state system.

  1992cited by this paper
• Electron microscopy of the actin-myosin head complex in the presence of ATP.

  1992cited by this paper
• Orientational disorder and motion of weakly attached cross-bridges.

  1991cited by this paper
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  1991cited by this paper
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  1989cited by this paper
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  1989cited by this paper
• X-ray study of myosin heads in contracting frog skeletal muscle.

  1987cited by this paper
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  1987cited by this paper
• New states of actomyosin.

  1987cited by this paper
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  1985cited by this paper
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  1985cited by this paper
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  1984cited by this paper
• The relation of muscle biochemistry to muscle physiology.

  1980cited by this paper
• Energetics and mechanism of actomyosin adenosine triphosphatase.

  1976cited by this paper
• Interactions of the actin and nucleotide binding sites on myosin subfragment 1.

  1976cited by this paper
• Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin

  1975cited by this paper
• Proposed Mechanism of Force Generation in Striated Muscle

  1971cited by this paper
• The Mechanism of Muscular Contraction

  1965cited by this paper

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  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2012cites this paper
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  2009cites this paper
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  2009cites this paper
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  2008cites this paper
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  2008cites this paper
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  2007cites this paper
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  2006cites this paper
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  2006cites this paper
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  2006cites this paper
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  2006cites this paper
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  2005cites this paper
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  2004cites this paper
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  2004cites this paper
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  2004cites this paper
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  2003cites this paper
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  2003cites this paper
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