Development of a microtiter plate-based assay for the detection of lipase-catalyzed transesterifications in organic solvents

A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of six organic solvents with logP values approximately between 1 and −1. This assay delivered results within comparatively short times measured by a color reaction and thus facilitates the choice of an enzyme-solvent combination for the synthesis of glycolipids. To validate the findings, glycolipid syntheses were performed using the same lipase formulation in the same solvents. When comparing the results obtained using the microtiter plate-based assay to the results of the glycolipid syntheses using the same lipases and solvents, matching results were obtained.

• Publication year

  2014

• Venue

  Biotechnology Letters

• Publication date

  2014-11-12

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1007/s10529-014-1721-0PMID 25388450PMCID 4342509
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution

  2013cited by this paper
• Methods for detection and characterization of lipases: A comprehensive review.

  2009cited by this paper
• A spectrophotometric transesterification-based assay for lipases in organic solvent.

  2009cited by this paper
• Screening methods for synthetic activity of lipases

  2007cited by this paper
• A modified para-nitrophenyl palmitate assay for lipase synthetic activity determination in organic solvent.

  2007cited by this paper
• Lipase-catalysed synthesis of glucose fatty acid esters in tert-butanol

  1999cited by this paper
• Effect of different biochemical parameters on the enzymatic synthesis of fructose oleate

  1996cited by this paper
• Hydrolysis of p-nitrophenyl palmitate in n-heptane by the Pseudomonas cepacia lipase: A simple test for the determination of lipase activity in organic media

  1996cited by this paper
• Kinetic resolutions of amine and thiol analogues of secondary alcohols catalyzed by the Candida antarctica lipase B

  1996cited by this paper
• Lipase-catalyzed syntheses of monoacylglycerols

  1995cited by this paper
• Simple screening method for lipase for transesterification in organic solvent

  1995cited by this paper
• A new assay for lipase activity in organic solvents. Lipase-catalyzed synthesis of octyl-linolenate in a hexane microaqueous reaction system

  1995cited by this paper
• Organic solvents strip water off enzymes

  1992cited by this paper
• Enzymatic catalysis in anhydrous organic solvents.

  1989cited by this paper

• Steady-state kinetic analysis of reversible enzyme inhibitors: A case study on calf intestine alkaline phosphatase.

  2023cites this paper
• Immobilization of lipase Lk2 and Lk3 on NiSiO3 microspheres and the application in the synthesis of methyl esters and β-sitosteryl esters

  2023cites this paper
• Molecular characterization of transesterification activity of novel lipase family I.1

  2022cites this paper
• New calogenin pregnane glycoside derivative from Huernia saudi- arabica and its Lipase and α-Glucosidase Inhibitory Activities.

  2020cites this paper
• A fluorescence-based activity assay for immobilized lipases in non-native media.

  2019cites this paper
• Lipase catalysed biodiesel synthesis with integrated glycerol separation in continuously operated microchips connected in series.

  2018cites this paper
• Synthesis of lipase polymer hybrids with retained or enhanced activity using the grafting-from strategy

  2018cites this paper
• Stability and Mobility of Lid Lipmnk in Acetonitrile by Molecular Dynamics Simulations Approach

  2018cites this paper
• Functional characterization of hormone sensitive-like lipase from Bacillus halodurans: synthesis and recovery of pNP-laurate with high yields

  2017cites this paper