AI-2-dependent gene regulation in Staphylococcus epidermidis

BackgroundAutoinducer 2 (AI-2), a widespread by-product of the LuxS-catalyzed S-ribosylhomocysteine cleavage reaction in the activated methyl cycle, has been suggested to serve as an intra- and interspecies signaling molecule, but in many bacteria AI-2 control of gene expression is not completely understood. Particularly, we have a lack of knowledge about AI-2 signaling in the important human pathogens Staphylococcus aureus and S. epidermidis.ResultsTo determine the role of LuxS and AI-2 in S. epidermidis, we analyzed genome-wide changes in gene expression in an S. epidermidis luxS mutant and after addition of AI-2 synthesized by over-expressed S. epidermidis Pfs and LuxS enzymes. Genes under AI-2 control included mostly genes involved in sugar, nucleotide, amino acid, and nitrogen metabolism, but also virulence-associated genes coding for lipase and bacterial apoptosis proteins. In addition, we demonstrate by liquid chromatography/mass-spectrometry of culture filtrates that the pro-inflammatory phenol-soluble modulin (PSM) peptides, key virulence factors of S. epidermidis, are under luxS/AI-2 control.ConclusionOur results provide a detailed molecular basis for the role of LuxS in S. epidermidis virulence and suggest a signaling function for AI-2 in this bacterium.

• Publication year

  2008

• Venue

  BMC Microbiology

• Publication date

  2008-01-08

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/1471-2180-8-4PMID 18182108PMCID 2241598
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Gram-positive three-component antimicrobial peptide-sensing system

  2007cited by this paper
• The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus

  2007cited by this paper
• Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA

  2007cited by this paper
• Autoinducer 2 Affects Biofilm Formation by Bacillus cereus

  2006cited by this paper
• Staphylococcus quorum sensing in biofilm formation and infection.

  2006cited by this paper
• Functional Analysis of luxS in Staphylococcus aureus Reveals a Role in Metabolism but Not Quorum Sensing

  2006influential reference
• Bacterial Small-Molecule Signaling Pathways

  2006cited by this paper
• Role of the luxS Quorum-Sensing System in Biofilm Formation and Virulence of Staphylococcus epidermidis

  2006influential reference
• YdgG (TqsA) Controls Biofilm Formation in Escherichia coli K-12 through Autoinducer 2 Transport

  2006cited by this paper
• Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2.

  2005cited by this paper
• Quorum sensing in Vibrio harveyi: probing the specificity of the LuxP binding site.

  2005cited by this paper
• Characterization of Type 2 Quorum Sensing in Klebsiella pneumoniae and Relationship with Biofilm Formation

  2005cited by this paper
• Making 'sense' of metabolism: autoinducer-2, LUXS and pathogenic bacteria

  2005cited by this paper
• Regulated expression of pathogen‐associated molecular pattern molecules in Staphylococcus epidermidis: quorum‐sensing determines pro‐inflammatory capacity and production of phenol‐soluble modulins

  2004cited by this paper
• Is autoinducer-2 a universal signal for interspecies communication: a comparative genomic and phylogenetic analysis of the synthesis and signal transduction pathways

  2004influential reference
• Virulence factors of the coagulase-negative staphylococci.

  2004cited by this paper
• Interspecies communication in bacteria.

  2003cited by this paper
• Mutation of luxS Affects Biofilm Formation in Streptococcus mutans

  2003cited by this paper
• Quorum-sensing control of biofilm factors in Staphylococcus epidermidis.

  2003cited by this paper
• Lsr‐mediated transport and processing of AI‐2 in Salmonella typhimurium

  2003cited by this paper
• LuxS-Based Signaling in Streptococcus gordonii: Autoinducer 2 Controls Carbohydrate Metabolism and Biofilm Formation with Porphyromonas gingivalis

  2003cited by this paper
• LuxS quorum sensing: more than just a numbers game.

  2003cited by this paper
• Quorum sensing and expression of virulence in Escherichia coli O157:H7.

  2003cited by this paper
• Death's toolbox: examining the molecular components of bacterial programmed cell death

  2003cited by this paper
• Mutation of luxS of Streptococcus pneumoniae Affects Virulence in a Mouse Model

  2003cited by this paper
• Quorum sensing in Campylobacter jejuni: detection of a luxS encoded signalling molecule.

  2002cited by this paper
• Staphylococcus epidermidis infections.

  2002cited by this paper
• LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone.

  2002cited by this paper
• The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum‐sensing signal molecule

  2001cited by this paper
• The LuxS‐dependent autoinducer AI‐2 controls the expression of an ABC transporter that functions in AI‐2 uptake in Salmonella typhimurium

  2001cited by this paper
• Mutation of luxS affects growth and virulence factor expression in Streptococcus pyogenes

  2001cited by this paper
• Construction and Characterization of anagr Deletion Mutant of Staphylococcus epidermidis

  2000cited by this paper
• Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production.

  1999cited by this paper
• An Inflammatory Polypeptide Complex from Staphylococcus epidermidis: Isolation and Characterization

  1999cited by this paper
• Staphylococcus epidermidis: emerging resistance and need for alternative agents.

  1998cited by this paper
• Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi

  1997cited by this paper
• Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin

  1994cited by this paper
• Staphylococcus epidermidis infections.

  1983cited by this paper

• Cu-Bearing Antibacterial Stainless Steel Potentiates Antibiotic Sensitivity to Combat Methicillin-resistant Staphylococcus aureus

  2025cites this paper
• Cooperation of quorum sensing and central carbon metabolism in the pathogenesis of Gram-positive bacteria.

  2024cites this paper
• The luxS deletion reduces the spoilage ability of Shewanella putrefaciens: An analysis focusing on quorum sensing and activated methyl cycle.

  2024cites this paper
• Quorum sensing gene regulation in Staphylococcus epidermidis reduces the attraction of Aedes aegypti (L.) (Diptera: Culicidae)

  2023cites this paper
• The role of RNA regulators, quorum sensing and c‐di‐GMP in bacterial biofilm formation

  2022cites this paper
• Biofilms by bacterial human pathogens: Clinical relevance - development, composition and regulation - therapeutical strategies

  2021cites this paper
• Staphylococcal Biofilm Development: Structure, Regulation, and Treatment Strategies

  2020cites this paper
• Proteomic Analysis Reveals a Biofilm-Like Behavior of Planktonic Aggregates of Staphylococcus epidermidis Grown Under Environmental Pressure/Stress

  2019cites this paper
• Qingre Baidu mixture-induced effect of AI-2 on Staphylococcus aureus and Pseudomonas aeruginosa biofilms in chronic and refractory wounds

  2019cites this paper
• Total alkaloids of Sophora alopecuroides and matrine inhibit auto-inducer 2 in the biofilms of Staphylococcus epidermidis.

  2019cites this paper
• Developing Anti-virulence Chemotherapies by Exploiting the Diversity of Microbial Quorum Sensing Systems

  2018cites this paper
• Biotechnological Applications of Quorum Sensing Inhibitors

  2018cites this paper
• Anti-Biofilm Activity of a Long-Chain Fatty Aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis Biofilm

  2017cites this paper
• Antibiofilm potential of 16-oxo-cleroda-3, 13(14) E-diene-15 oic acid and its five new γ-amino γ-lactone derivatives against methicillin resistant Staphylococcus aureus and Streptococcus mutans.

  2017cites this paper
• Autoinducer 2 Signaling via the Phosphotransferase FruA Drives Galactose Utilization by Streptococcus pneumoniae, Resulting in Hypervirulence

  2017cites this paper
• Novel roles of staphylococcal proteases and cross talk in biofilm formation and virulence

  2016cites this paper
• Compounds from Natural Sources for New Diagnostics and Drugs against Biofilm Infections

  2016cites this paper
• The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response

  2016cites this paper
• Dual Quorum Quenching Capsules: Disrupting two bacterial communication pathways that lead to virulence

  2016cites this paper
• Quorum-sensing regulation in staphylococci—an overview

  2015cites this paper
• Autoinducer-2 increases biofilm formation via an ica- and bhp-dependent manner in Staphylococcus epidermidis RP62A.

  2015cites this paper
• Inhibition of Quorum Sensing in Staphylococcus spp.

  2015cites this paper
• Contextualization of the E. coli Lsr system: Relative orthology, relative QS activity, and emergent behavior

  2015cites this paper
• Geminal dihalogen isosteric replacement in hydrated AI-2 affords potent quorum sensing modulators.

  2015cites this paper
• Investigational therapies targeting quorum-sensing for the treatment of Staphylococcus aureus infections

  2015cites this paper
• Staphylococcus Epidermidis

  2014cites this paper
• AI-2 communication in Staphylococcus epidermidis: A study on possible effects of thiophenones

  2014cites this paper
• Biofilm as a Multicellular Bacterial System

  2014cites this paper
• Analysis of gene expression variability in Staphylococcus epidermidis biofilms

  2014cites this paper
• Phenol-soluble modulins: novel virulence-associated peptides of staphylococci.

  2014cites this paper
• Staphylococcus epidermidis pathogenesis.

  2014cites this paper
• In –silico molecular docking analysis of prodigiosin and cycloprodigiosin as COX-2 inhibitors

  2013cites this paper
• Anti-biofilm activity of the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125.

  2013cites this paper
• Characterization of the molecular interactions between Staphylococcus epidermidis biofilm infections and the host immune system

  2013influential citation
• Evaluating the Co-commensal Lifestyles of Staphylococcus aureus and Staphylococcus epidermidis

  2013cites this paper
• AI-2-mediated signalling in bacteria.

  2013cites this paper
• Biofilms are a natural part of the ecology of the Earth , and correspond to a ‘ social structure ’ of microorganisms compared with a planktonic state

  2012cites this paper
• Analysis of bacterial biofilms using NMR-based metabolomics.

  2012cites this paper
• Fatty Acid Modulation of Autoinducer (AI-2) Influenced Growth and Macrophage Invasion by Salmonella Typhimurium

  2012cites this paper
• Novel Phenol-soluble Modulin Derivatives in Community-associated Methicillin-resistant Staphylococcus aureus Identified through Imaging Mass Spectrometry*

  2012cites this paper
• Detection of AI-2 Receptors in Genomes of Enterobacteriaceae Suggests a Role of Type-2 Quorum Sensing in Closed Ecosystems

  2012cites this paper
• Molecular basis of Staphylococcus epidermidis infections

  2012cites this paper
• Optimizing a qPCR Gene Expression Quantification Assay for S. epidermidis Biofilms: A Comparison between Commercial Kits and a Customized Protocol

  2012cites this paper
• Genetic regulation of the intercellular adhesion locus in staphylococci

  2012cites this paper
• Thiophenones inhibit Staphylococcus epidermidis biofilm formation at nontoxic concentrations.

  2012influential citation
• EVALUATION OF THE EXPRESSION OF LUXS GENE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS FROM BLOODSTREAM INFECTIONS

  2012cites this paper
• Novel Phenol-soluble Modulin Derivatives in Community-associated Methicillin-resistantStaphylococcus

  2012cites this paper
• Microbial biofilms on peritoneal dialysis catheters

  2011cites this paper
• Microbes and Microbial Technology

  2011cites this paper
• Furanones and Thiophenones in Control of Staphylococcus epidermidis Biofilm Infections

  2011cites this paper
• Methylthioadenosine/S‐adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism

  2011cites this paper
• Population level virulence in polymicrobial communities associated with chronic disease

  2011cites this paper
• Cell-Cell communication in Streptococcus mutans – From a global view to the single cell perspective

  2011cites this paper
• The importance of a multifaceted approach to characterizing the microbial flora of chronic wounds

  2011cites this paper
• Bacterial Quorum Sensing and Its Interference: Methods and Significance

  2011cites this paper
• A Microfluidic Programmable Array for Label-free Detection of Biomolecules

  2011cites this paper
• Molecular Biology of Staphylococcal Pathogenesis

  2010cites this paper
• Staphylococcus aureus AI-2 Quorum Sensing Associates with the KdpDE Two-Component System To Regulate Capsular Polysaccharide Synthesis and Virulence

  2010cites this paper
• Virulence Polysaccharide Synthesis and To Regulate Capsular the KdpDE Two-Component System Quorum Sensing Associates with AI-2 Staphylococcus aureus

  2010cites this paper
• Biofilm formation and antimicrobial resistance of coagulase-negative Staphylococci isolated from neonatal intensive care units

  2010influential citation
• VirulenceSynthesis and To Regulate Capsular the KdpDE Two-Component System Quorum Sensing Associates with AI-2 Staphylococcus aureus

  2010cites this paper
• Current concepts in biofilm formation of Staphylococcus epidermidis.

  2010cites this paper
• Enzyme–ligand interactions that drive active site rearrangements in the Helicobacter pylori 5′‐methylthioadenosine/S‐adenosylhomocysteine nucleosidase

  2010cites this paper
• Cell–Cell Communication and Biofilm Formation in Gram‐Positive Bacteria

  2009cites this paper
• 1 Cell – Cell Communication and Biofilm Formation in Gram-Positive Bacteria

  2009cites this paper
• Synthetic furanones and inhibition of biofilm formation

  2009cites this paper
• The Crystal Structure of the Escherichia coli Autoinducer-2 Processing Protein LsrF

  2009cites this paper
• Identification of Functional LsrB-Like Autoinducer-2 Receptors

  2009cites this paper
• Communication between microorganisms as a basis for production of virulence factors.

  2009cites this paper
• Furanone at Subinhibitory Concentrations Enhances Staphylococcal Biofilm Formation by luxS Repression

  2009cites this paper
• Molecular mechanisms involved in biofilm formation by food-associated bacteria

  2009cites this paper
• Characterisation of Persister Cells in Biofilms and Planktonic Bacterial Populations

  2009cites this paper
• RNAIII-independent target gene control by the agr quorum-sensing system: insight into the evolution of virulence regulation in Staphylococcus aureus.

  2008cites this paper
• Quorum Sensing by the Bacterial Signal Autoinducer-2: Phylogenetic Distribution of the Synthesis Gene LuxS, and its Role in Shewanella oneidensis

  2008cites this paper
• Sinorhizobium meliloti, a bacterium lacking the autoinducer‐2 (AI‐2) synthase, responds to AI‐2 supplied by other bacteria

  2008cites this paper
• Furanones, potential agents for preventing Staphylococcus epidermidis biofilm infections?

  2008cites this paper
• Bench-to-bedside review: Quorum sensing and the role of cell-to-cell communication during invasive bacterial infection

  2008cites this paper
• Quorum sensing in staphylococci.

  2008cites this paper