Multiplex Gene Expression Profiling of 16 Target Genes in Neoplastic and Non-Neoplastic Canine Mammary Tissues Using Branched-DNA Assay

Mammary gland tumors are one of the most common neoplasms in female dogs, and certain breeds are prone to develop the disease. The use of biomarkers in canines is still restricted to research purposes. Therefore, the necessity to analyze gene profiles in different mammary entities in large sample sets is evident in order to evaluate the strength of potential markers serving as future prognostic factors. The aim of the present study was to analyze the gene expression of 16 target genes (BRCA1, BRCA2, FOXO3, GATA4, HER2, HMGA1, HMGA2, HMGB1, MAPK1, MAPK3, MCL1, MYC, PFDN5, PIK3CA, PTEN, and TP53) known to be involved in human and canine mammary neoplasm development. Expression was analyzed in 111 fresh frozen (FF) and in 170 formalin-fixed, paraffin-embedded (FFPE) specimens of neoplastic and non-neoplastic canine mammary tissues using a multiplexed branched-DNA (b-DNA) assay. TP53, FOXO3, PTEN, and PFDN5 expression revealed consistent results with significant low expression in malignant tumors. The possibility of utilizing them as predictive factors as well as for assisting in the choice of an adequate gene therapy may help in the development of new and improved approaches in canine mammary tumors.

• Publication year

  2016

• Venue

  International Journal of Molecular Sciences

• Publication date

  2016-09-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3390/ijms17091589PMID 27657059PMCID 5037854
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A Physical Mechanism and Global Quantification of Breast Cancer

  2016cited by this paper
• A Comparison of Fresh Frozen vs. Formalin-Fixed, Paraffin-Embedded Specimens of Canine Mammary Tumors via Branched-DNA Assay

  2016cited by this paper
• High mobility group box-1 and its clinical value in breast cancer

  2015cited by this paper
• Canine Mammary Tumours Are Affected by Frequent Copy Number Aberrations, including Amplification of MYC and Loss of PTEN

  2015influential reference
• [Expression of PTEN, p53 and EGFR in the molecular subtypes of breast carcinoma and 
the correlation among them].

  2015cited by this paper
• Prevalence of the Prefoldin Subunit 5 Gene Deletion in Canine Mammary Tumors

  2015cited by this paper
• Epidemiologie

  2014cited by this paper
• Genome Aberrations in Canine Mammary Carcinomas and Their Detection in Cell-Free Plasma DNA

  2013cited by this paper
• Classification and grading of canine malignant mammary tumors

  2013cited by this paper
• Genetic variations of BRCA1 and BRCA2 genes in dogs with mammary tumours

  2013cited by this paper
• Foxo3a Expression Is a Prognostic Marker in Breast Cancer

  2013cited by this paper
• Breed-Predispositions to Cancer in Pedigree Dogs

  2013cited by this paper
• Breast cancer diagnostics using microfluidic multiplexed immunohistochemistry.

  2013cited by this paper
• The impact of p53 in predicting clinical outcome of breast cancer patients with visceral metastasis

  2013cited by this paper
• A Review on Breast Cancer Biomarkers BRCA1 and BRCA2

  2012cited by this paper
• Applications of immunohistochemistry

  2012cited by this paper
• Postoperative treatment outcome in canine mammary tumors. Multivariate analysis of the prognostic value of pre- and postoperatively available information.

  2012cited by this paper
• Consensus for the diagnosis, prognosis and treatment of feline mammary tumors

  2011cited by this paper
• Genetic testing for familial/hereditary breast cancer—comparison of guidelines and recommendations from the UK, France, the Netherlands and Germany

  2011cited by this paper
• Molecular Biological Aspects on Canine and Human Mammary Tumors

  2011cited by this paper
• Canine mammary tumours as a model to study human breast cancer: most recent findings.

  2011cited by this paper
• Data from the Danish Veterinary Cancer Registry on the occurrence and distribution of neoplasms in dogs in Denmark

  2010cited by this paper
• Breast Cancer Diagnosis Using a Microfluidic Multiplexed Immunohistochemistry Platform

  2010cited by this paper
• Genetic susceptibility to breast cancer

  2010cited by this paper
• Effectiveness of small interfering RNA (siRNA) against the Mcl-1 gene in a canine mammary gland tumor cell line.

  2009influential reference
• Gene and chromosomal alterations in sporadic breast cancer: correlation with histopathological features and implications for genesis and progression

  2009cited by this paper
• Mammary tumor development in dogs is associated with BRCA1 and BRCA2.

  2009cited by this paper
• Increased Expression of BRCA2 and RAD51 in Lymph Node Metastases of Canine Mammary Adenocarcinomas

  2009cited by this paper
• Increased survival in dogs with malignant mammary tumours overexpressing HER-2 protein and detection of a silent single nucleotide polymorphism in the canine HER-2 gene.

  2009influential reference
• Reduced PTEN Protein Expression and Its Prognostic Implications in Canine and Feline Mammary Tumors

  2009cited by this paper
• Expression and significance of PTEN in canine mammary gland tumours.

  2008cited by this paper
• Evaluation of the branched-chain DNA assay for measurement of RNA in formalin-fixed tissues.

  2008cited by this paper
• Roles of HMGA proteins in cancer

  2007cited by this paper
• A multiplex branched DNA assay for parallel quantitative gene expression profiling.

  2006cited by this paper
• Expression of the PTEN tumor suppressor gene in malignant mammary gland tumors of dogs.

  2006cited by this paper
• Histological grading and prognosis in dogs with mammary carcinomas: application of a human grading method.

  2005cited by this paper
• Frequent Mutation of the PIK3CA Gene in Ovarian and Breast Cancers

  2005cited by this paper
• Expression and prognostic relevance of Mcl-1 in breast cancer.

  2004cited by this paper
• HER-2/neu Gene and protein in breast cancer

  2004cited by this paper
• FoxOs at the crossroads of cellular metabolism, differentiation, and transformation.

  2004cited by this paper
• BRCA1 expression in canine mammary dysplasias and tumours: relationship with prognostic variables.

  2003cited by this paper
• The Her-2/neu gene and protein in breast cancer 2003: biomarker and target of therapy.

  2003cited by this paper
• Reduced expression of PTEN correlates with breast cancer progression.

  2002cited by this paper
• The p53 pathway in breast cancer

  2002cited by this paper
• MM-1, a c-Myc-binding Protein, Is a Candidate for a Tumor Suppressor in Leukemia/Lymphoma and Tongue Cancer*

  2001cited by this paper
• Cloning and sequencing full length of canine Brca2 and Rad51 cDNA.

  2001cited by this paper
• Interpretation and Quantification of Immunostains

  2001cited by this paper
• p53 Gene Mutations Occurring in Spontaneous Benign and Malignant Mammary Tumors of the Dog

  2000influential reference
• Genomic organization of the canine p53 gene and its mutational status in canine mammary neoplasia

  1998influential reference
• A study of the PTEN/MMAC1 gene in 136 breast cancer families.

  1998cited by this paper
• PTEN, a Putative Protein Tyrosine Phosphatase Gene Mutated in Human Brain, Breast, and Prostate Cancer

  1997cited by this paper
• Hyperexpression of mitogen-activated protein kinase in human breast cancer.

  1997cited by this paper
• P53 mutations in mammary tumor cell lines and corresponding tumor tissues in the dog.

  1996cited by this paper
• Canine and feline mammary neoplasms.

  1986cited by this paper
• Biomolecular Detection and Quantification

  year unknowncited by this paper

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  2024cites this paper
• Current invasive and non-invasive biomarkers in canine mammary tumors

  2021cites this paper
• VEGF-C and p53 Gene Expression in the Normal and Neoplastic Mammary Gland of Canines: A Pilot Study

  2021cites this paper
• Mutations of BRCA2 in canine mammary tumors and their targeting potential in clinical therapy

  2020cites this paper
• The functions and mechanisms of prefoldin complex and prefoldin-subunits

  2020cites this paper