Growth, fatty acid profile in major lipid classes and lipid fluidity of Aurantiochytrium mangroveiSK-02 As a function of growth temperature

Aurantiochytrium mangrovei Sk-02 was grown in a medium containing glucose (40 g/l), yeast extract (10 g/L) and sea salts (15 g/L) at temperatures ranging from 12 to 35°C. The fastest growth (µmax= 0.15 h-1) and highest fatty acid content of 415 mg/g-dry cell weight were found in the cells grown at 30°C. However, the cells grown at 12°C showed the highest percentage of polyunsaturated fatty acid (PUFA) (48.6% of total fatty acid). The percentage of docosahexaenoic acid (DHA) and pentadecanoic acid (C15:0) decreased with an increase in the growth temperature, whereas, palmitic acid (C16:0), stearic acid (C18:0) and DPA (C22:5n6) increased with an increase in the growth temperature. The composition of the major lipid class (%w/w) was slightly affected by the growth temperature. The fluidity of the organelle membrane or intracellular lipid (by DPH measurement) decreased with an increase in the growth temperatures, while the plasma membrane fluidity (by TMA-DPH measurement) could still maintain its fluidity in a wide range of temperatures (15 - 37°C). Furthermore, the distribution of DHA was found to be higher (36 – 54%) in phospholipid (PL) as compared to neutral lipid (NL) (20 - 41%).

• Publication year

  2012

• Venue

  Brazilian Journal of Microbiology

• Publication date

  2012-06-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1590/S1517-838220120001000020PMID 24031817PMCID 3768996
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• All the content of the journal, except where otherwise noted, is licensed under a Creative

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