Structural basis for chiral substrate recognition by two 2,3‐butanediol dehydrogenases

2,3‐Butanediol dehydrogenase (BDH) catalyzes the NAD‐dependent redox reaction between acetoin and 2,3‐butanediol. There are three types of homologous BDH, each stereospecific for both substrate and product. To establish how these homologous enzymes possess differential stereospecificities, we determined the crystal structure of l‐BDH with a bound inhibitor at 2.0 Å. Comparison with the inhibitor binding mode of meso‐BDH highlights the role of a hydrogen‐bond from a conserved Trp residue192. Site‐directed mutagenesis of three active site residues of meso‐BDH, including Trp190, which corresponds to Trp192 of l‐BDH, converted its stereospecificity to that of l‐BDH. This result confirms the importance of conserved residues in modifying the stereospecificity of homologous enzymes.

• Publication year

  2010

• Venue

  FEBS Letters

• Publication date

  2010-01-04

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.febslet.2009.11.068PMID 19941855
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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