SCIENCE@home

The Protein Trio RPK1-CaM4-RbohF Mediates Transient Superoxide Production to Trigger Age-Dependent Cell Death in Arabidopsis.

J. Koo,I. C. Lee,C. Dai,Yuree Lee,H. Cho,Yumi Kim,Bong‐Kwan Phee,Hyunmin Kim,Il Hwan Lee,Seung Hee Choi,S. J. Park,In Seon Jeon,H. Nam,J. Kwak

Published 2017 in Cell Reports

ABSTRACT

Reactive oxygen species (ROS) are inevitable by-products of aerobic metabolic processes, causing non-specific oxidative damage and also acting as second messengers. Superoxide is a short-lived ROS that functions in various cellular responses, including aging and cell death. However, it is unclear as to how superoxide brings about age-dependent cell death and senescence. Here, we show that the accumulation and signaling of superoxide are mediated by three Arabidopsis proteins-RPK1, CaM4, and RbohF-which trigger subsequent cellular events leading to age-dependent cell death. We demonstrate that the NADPH oxidase RbohF is responsible for RPK1-mediated transient accumulation of superoxide, SIRK kinase induction, and cell death, all of which are positively regulated by CaM4. RPK1 physically interacts with and phosphorylates CaM4, which, in turn, interacts with RbohF. Overall, we demonstrate how the protein trio governs the superoxide accumulation and signaling at the cell surface to control senescence and cell death.

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-47 of 47 references · Page 1 of 1

CITED BY

Showing 1-34 of 34 citing papers · Page 1 of 1