Serum- and glucocorticoid-induced kinase 3 in recycling endosomes mediates acute activation of Na+/H+ exchanger NHE3 by glucocorticoids

SGK1 plays an important role in regulation of Na+/H+ exchanger (NHE) 3 in vivo. We show that SGK3 colocalizes with NHE3 in recycling endosomes. These studies identify SGK3 as the effector of the PI3K pathway that activates NHE3 and show that endosomal localization of SGK3 is essential for acute activation of NHE3.

• Publication year

  2011

• Venue

  Molecular Biology of the Cell

• Publication date

  2011-10-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E11-04-0328PMID 21865597PMCID 3192861
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• SGK3 is an estrogen-inducible kinase promoting estrogen-mediated survival of breast cancer cells.

  2011cited by this paper
• Lysophosphatidic acid stimulates the intestinal brush border Na(+)/H(+) exchanger 3 and fluid absorption via LPA(5) and NHERF2.

  2010cited by this paper
• Expression and role of serum and glucocorticoid-regulated kinase 2 in the regulation of Na+/H+ exchanger 3 in the mammalian kidney.

  2010cited by this paper
• Endogenous glucocorticoids and impaired insulin signaling are both required to stimulate muscle wasting under pathophysiological conditions in mice.

  2009cited by this paper
• The absence of LPA2 attenuates tumor formation in an experimental model of colitis-associated cancer.

  2009cited by this paper
• Mechanisms of the Regulation of the Intestinal Na+/H+Exchanger NHE3

  2009cited by this paper
• IRBIT, Inositol 1,4,5-Triphosphate (IP3) Receptor-binding Protein Released with IP3, Binds Na+/H+ Exchanger NHE3 and Activates NHE3 Activity in Response to Calcium*

  2008cited by this paper
• Endothelial Nitric Oxide Synthase

  2008cited by this paper
• Epithelial Na+ channel activation and processing in mice lacking SGK1.

  2008cited by this paper
• Acute activation of NHE3 by dexamethasone correlates with activation of SGK1 and requires a functional glucocorticoid receptor.

  2007cited by this paper
• Characterization of the regulation of renal Na+/H+ exchanger NHE3 by insulin.

  2007cited by this paper
• Identification and characterization of a constitutively T-loop phosphorylated and active recombinant S6K1: expression, purification, and enzymatic studies in a high capacity non-radioactive TR-FRET Lance assay.

  2006cited by this paper
• The Serum and Glucocorticoid Inducible Kinases SGK1-3 Stimulate the Neutral Amino Acid Transporter SLC6A19

  2006cited by this paper
• Role of the Phox Homology Domain and Phosphorylation in Activation of Serum and Glucocorticoid-regulated Kinase-3*

  2006influential reference
• CISK attenuates degradation of the chemokine receptor CXCR4 via the ubiquitin ligase AIP4

  2006cited by this paper
• (Patho)physiological significance of the serum- and glucocorticoid-inducible kinase isoforms.

  2006cited by this paper
• Intestinal function of gene-targeted mice lacking serum- and glucocorticoid-inducible kinase 1.

  2006cited by this paper
• G-protein-coupled glucocorticoid receptors on the pituitary cell membrane

  2005cited by this paper
• Activation of NHE3 by dexamethasone requires phosphorylation of NHE3 at Ser663 by SGK1.

  2005cited by this paper
• Glucocorticoid adrenal steroids and glucocorticoid‐inducible kinase isoforms in the regulation of GluR6 expression

  2005cited by this paper
• Regulation of GluR1 abundance in murine hippocampal neurones by serum‐ and glucocorticoid‐inducible kinase 3

  2005cited by this paper
• Targeted disruption of the protein kinase SGK3/CISK impairs postnatal hair follicle development.

  2004cited by this paper
• Regulation of the Epithelial Ca2+ Channel TRPV5 by the NHE Regulating Factor NHERF2 and the Serum and Glucocorticoid Inducible Kinase Isoforms SGK1 and SGK3 Expressed in Xenopus oocytes

  2004cited by this paper
• Endothelial Nitric Oxide Synthase: A New Paradigm for Gene Regulation in the Injured Blood Vessel

  2004cited by this paper
• The serine/threonine kinases SGK2 and SGK3 are potent stimulators of the epithelial Na+ channel alpha,beta,gamma-ENaC.

  2003cited by this paper
• Sorting out the cellular functions of sorting nexins

  2003cited by this paper
• Lysophosphatidic Acid Stimulates Brush Border Na+/H+ Exchanger 3 (NHE3) Activity by Increasing Its Exocytosis by an NHE3 Kinase A Regulatory Protein-dependent Mechanism*

  2003cited by this paper
• Serum and Glucocorticoid Inducible Kinase

  2003cited by this paper
• The serine/threonine kinases SGK2 and SGK3 are potent stimulators of the epithelial Na+ channel α,β,γ-ENaC

  2003cited by this paper
• Regulation of the glutamate transporter EAAT1 by the ubiquitin ligase Nedd4‐2 and the serum and glucocorticoid‐inducible kinase isoforms SGK1/3 and protein kinase B

  2003cited by this paper
• Hormone-regulated transepithelial Na+ transport in mammalian CCD cells requires SGK1 expression.

  2003cited by this paper
• Regulation of Channels by the Serum and Glucocorticoid-Inducible Kinase - Implications for Transport, Excitability and Cell Proliferation

  2003cited by this paper
• Nongenomic actions of steroid hormones

  2003cited by this paper
• The serum and glucocorticoid-inducible kinase SGK1 and the Na+/H+ exchange regulating factor NHERF2 synergize to stimulate the renal outer medullary K+ channel ROMK1.

  2002cited by this paper
• Acute cardiovascular protective effects of corticosteroids are mediated by non-transcriptional activation of endothelial nitric oxide synthase

  2002cited by this paper
• Impaired renal Na(+) retention in the sgk1-knockout mouse.

  2002cited by this paper
• Regulation of KCNE1-dependent K+ current by the serum and glucocorticoid-inducible kinase (SGK) isoforms

  2002cited by this paper
• Activation of Na+/K+-ATPase by the Serum and Glucocorticoid-Dependent Kinase Isoforms

  2002cited by this paper
• Sorting out the cellular functions of sorting nexins

  2002cited by this paper
• Glucocorticoid Activation of Na+/H+Exchanger Isoform 3 Revisited

  2002cited by this paper
• IGF-1 up-regulates K+ channels via PI3-kinase, PDK1 and SGK1

  2002cited by this paper
• Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids.

  2002cited by this paper
• Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate

  2001cited by this paper
• Regulation of cytokine-independent survival kinase (CISK) by the Phox homology domain and phosphoinositides

  2001cited by this paper
• Phosphorylation of Nedd4‐2 by Sgk1 regulates epithelial Na+ channel cell surface expression

  2001cited by this paper
• Basic Fibroblast Growth Factor Stimulates Surface Expression and Activity of Na+/H+ Exchanger NHE3 via Mechanism Involving Phosphatidylinositol 3-Kinase*

  2000cited by this paper
• sgk Is an Aldosterone-induced Kinase in the Renal Collecting Duct

  1999cited by this paper
• Serum and glucocorticoid‐inducible kinase (SGK) is a target of the PI 3‐kinase‐stimulated signaling pathway

  1999cited by this paper
• The Serum and Glucocorticoid Kinase sgk Increases the Abundance of Epithelial Sodium Channels in the Plasma Membrane of Xenopus Oocytes*

  1999cited by this paper
• Regulatory Sequences of the Mouse Villin Gene That Efficiently Drive Transgenic Expression in Immature and Differentiated Epithelial Cells of Small and Large Intestines*

  1999cited by this paper
• Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase.

  1999influential reference
• Endosomal Recycling of the Na+/H+Exchanger NHE3 Isoform Is Regulated by the Phosphatidylinositol 3-Kinase Pathway*

  1998cited by this paper
• The Role of NHERF and E3KARP in the cAMP-mediated Inhibition of NHE3*

  1998influential reference
• The Epithelial Sodium-Hydrogen Antiporter Na+/H+ Exchanger 3 Accumulates and Is Functional in Recycling Endosomes*

  1998cited by this paper
• Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney.

  1997cited by this paper
• Oral budesonide is as effective as oral prednisolone in active Crohn’s disease

  1997cited by this paper
• Novel domains in NADPH oxidase subunits, sorting nexins, and PtdIns 3‐kinases: Binding partners of SH3 domains?

  1996cited by this paper
• Effect of glucocorticoids on renal cortical NHE-3 and NHE-1 mRNA.

  1994cited by this paper
• Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum

  1993cited by this paper
• Glucocorticoid stimulation of ileal Na+ absorptive cell brush border Na+/H+ exchange and association with an increase in message for NHE-3, an epithelial Na+/H+ exchanger isoform.

  1993cited by this paper
• The Biology of the Cell

  1981cited by this paper
• Na+-K+-activated adenosine triphosphatase and intestinal electrolyte transport. Effect of adrenal steroids.

  1975cited by this paper
• Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Upregulation of Herg Channels by the Serum and Glucocorticoid Inducible Kinase Isoform Sgk3

  year unknowncited by this paper
• Activation of Na (cid:1) /H (cid:1) Exchanger NHE3 by Angiotensin II Is Mediated by Inositol 1,4,5-Triphosphate (IP 3 ) Receptor-binding Protein Released with IP 3 (IRBIT) and Ca 2 (cid:1) /Calmodulin-dependent Protein Kinase II *

  year unknowncited by this paper

• Histamine regulates the activity and the expression of the Na+/H+ exchanger (NHE)3 in human epithelial HK-2 cells

  2025cites this paper
• Serum- and glucocorticoid-induced kinase 3 orchestrates glucocorticoid signaling to facilitate chromatin remodeling during murine adipogenesis

  2025cites this paper
• Dexamethasone disrupts intracellular pH homeostasis to delay coronavirus infectious bronchitis virus cell entry via sodium hydrogen exchanger 3 activation

  2025influential citation
• The mTORC2 signaling network: targets and cross-talks

  2024cites this paper
• Dexamethasone Upregulates the Expression of the Human SLC26A3 (DRA, Down-Regulated in Adenoma) Transporter (an IBD Susceptibility Gene) in Intestinal Epithelial Cells and Attenuates Gut Inflammation.

  2024cites this paper
• VPS34-IN1 inhibits cap-mediated translation and synergizes with STING to drive type-I IFN expression in human plasmacytoid DCs

  2024cites this paper
• Molecular Role of SGK3 Gene in Promoting the Progression of Non-small Cell Lung Cancer

  2024cites this paper
• Regulation of distal tubule sodium transport: mechanisms and roles in homeostasis and pathophysiology

  2022cites this paper
• Contributions of SGK3 to transporter-related diseases

  2022cites this paper
• The Mechanism of PTOV1 Regulation by 14-3-3, HUWEI1 and SGK2

  2022cites this paper
• Kidney ion handling genes and their interaction in blood pressure control

  2022cites this paper
• Metformin Inhibits Na+/H+ Exchanger NHE3 Resulting in Intestinal Water Loss

  2022cites this paper
• Serum and Glucocorticoid-Inducible Kinase 3/Nedd4-2 Signaling Pathway Participates in Podocyte Injury by Regulating the Stability of Nephrin

  2022cites this paper
• The recycling regulation of sodium-hydrogen exchanger isoform 3(NHE3) in epithelial cells

  2021cites this paper
• Nedd4-2–dependent Ubiquitination Potentiates the Inhibition of Human NHE3 by Cholera Toxin and Enteropathogenic Escherichia coli

  2021cites this paper
• Geroprotective properties of neuroprotective and neurotrophic peptides

  2020cites this paper
• Na+/H+ Exchangers in Epithelia

  2020cites this paper
• Angiotensin II inhibits P‐glycoprotein in intestinal epithelial cells

  2020cites this paper
• Oocyte Maturation in Starfish

  2020cites this paper
• Serum- and glucocorticoid- inducible kinase 2, SGK2, is a novel autophagy regulator and modulates platinum drugs response in cancer cells

  2020cites this paper
• SGK regulates pH increase and cyclin B–Cdk1 activation to resume meiosis in starfish ovarian oocytes

  2019cites this paper
• The SLC9A-C Mammalian Na+/H+ Exchanger Family: Molecules, Mechanisms, and Physiology.

  2019cites this paper
• Physiology of Electrolyte Transport in the Gut: Implications for Disease.

  2019cites this paper
• SGK regulates pH increase and cyclin B-Cdk1 activation to resume meiosis in starfish ovarian 1 oocytes 2

  2018cites this paper
• SLC9 Gene Family: Function, Expression, and Regulation.

  2018cites this paper
• Expression of lysophosphatidic acid receptor 5 is necessary for the regulation of intestinal Na+/H+ exchanger 3 by lysophosphatidic acid in vivo.

  2018cites this paper
• SGK regulates pH increase and cyclin B-Cdk1 activation to resume meiosis in starfish ovarian oocytes

  2018cites this paper
• Two Liters a Day Keep the Doctor Away? Considerations on the Pathophysiology of Suboptimal Fluid Intake in the Common Population

  2017cites this paper
• Associations of the Serum/Glucocorticoid Regulated Kinase Genes With BP Changes and Hypertension Incidence: The Gensalt Study

  2017cites this paper
• The C-terminal MIR-containing region in the Pmt1 O-mannosyltransferase restrains sporulation and is dispensable for virulence in Beauveria bassiana

  2017cites this paper
• Regulation of Transporters and Channels by Membrane-Trafficking Complexes in Epithelial Cells.

  2017cites this paper
• Structural Basis Of Membrane Protein Scaffolding And Signaling In Human Disease

  2017cites this paper
• Serum and Glucocorticoid Regulated Kinase 1 in Sodium Homeostasis

  2016cites this paper
• The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II.

  2016cites this paper
• Role of epithelial ion transports in inflammatory bowel disease.

  2016cites this paper
• Targets and Off-Targets of Kinase Inhibitors in Diabetes

  2015cites this paper
• Krüppel-like factor 5 incorporates into the β-catenin/TCF complex in response to LPA in colon cancer cells.

  2015cites this paper
• Regulation of NHE3 by lysophosphatidic acid is mediated by phosphorylation of NHE3 by RSK2.

  2015cites this paper
• Regulation of NHE 3 by lysophosphatidic acid is mediated by phosphorylation of NHE 3 by RSK 2

  2015cites this paper
• PDZ Structure and Implication in Selective Drug Design against Cystic Fibrosis.

  2015cites this paper
• HIF1α-Induced by Lysophosphatidic Acid Is Stabilized via Interaction with MIF and CSN5

  2015cites this paper
• Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss.

  2015cites this paper
• Structural insights into PDZ-mediated interaction of NHERF2 and LPA(2), a cellular event implicated in CFTR channel regulation.

  2014cites this paper
• Relevance of SGK1 in structural, functional and molecular alterations produced by aldosterone in heart

  2014cites this paper
• Unique Regulation of Human Na+/H+ Exchanger 3 (NHE3) by Nedd4-2 Ligase That Differs from Non-primate NHE3s*

  2014cites this paper
• SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1.

  2014cites this paper
• Cyclic GMP Kinase II (cGKII) Inhibits NHE3 by Altering Its Trafficking and Phosphorylating NHE3 at Three Required Sites

  2014cites this paper
• Regulation of transport across cell membranes by the serum- and glucocorticoid-inducible kinase SGK1

  2014cites this paper
• AKT and GSK-3 Are Necessary for Direct Ezrin Binding to NHE3 as Part of a C-terminal Stimulatory Complex

  2014cites this paper
• Control of Intracellular pH

  2013cites this paper
• Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants

  2013cites this paper
• Traditional and emerging roles for the SLC9 Na+/H+ exchangers

  2013cites this paper
• Sgk1-Dependent Stimulation of Cardiac Na+/H+ Exchanger Nhe1 by Dexamethasone

  2013cites this paper
• Serum and glucocorticoid inducible kinase, metabolic syndrome, inflammation, and tumor growth

  2013cites this paper
• Therapeutic potential of serum and glucocorticoid inducible kinase inhibition

  2013influential citation
• Distinct Phospholipase C-β Isozymes Mediate Lysophosphatidic Acid Receptor 1 Effects on Intestinal Epithelial Homeostasis and Wound Closure

  2013cites this paper
• Regulation of Hypoxia-inducible Factor 1α (HIF-1α) by Lysophosphatidic Acid Is Dependent on Interplay between p53 and Krüppel-like Factor 5*

  2013cites this paper
• Human intestinal epithelial cell line SK-CO15 is a new model system to study Na(+)/H(+) exchanger 3.

  2012cites this paper
• SGK regulation of renal sodium transport

  2012cites this paper
• How yeast re-programmes its transcriptional profile in response to different nutrient impulses

  2011cites this paper
• Regulation of Hypoxia-inducible Factor 1 alpha (HIF-1 alpha) by Lysophosphatidic Acid Is Dependent on Interplay between p53 and Kruppel-like Factor 5

  year unknowncites this paper
• HIF 1 α-Induced by Lysophosphatidic Acid Is Stabilized via Interaction with MIF and CSN 5

  year unknowncites this paper