Efficient preparation and properties of mRNAs containing a fluorescent cap analog: Anthraniloyl-m7GpppG

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Translation

• Publication date

  2015-01-02

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.4161/21690731.2014.988538PMID 26779415PMCID PMC4682804
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1999cited by this paper
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  1998cited by this paper
• Viral and cellular enzymes involved in synthesis of mRNA cap structure.

  1997cited by this paper
• Participation of the nuclear cap binding complex in pre-mRNA 3' processing.

  1997cited by this paper
• X-ray studies of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP.

  1997cited by this paper
• Synthesis of a fluorescent 7-methylguanosine analog and a fluorescence spectroscopic study of its reaction with wheatgerm cap binding proteins.

  1996influential reference
• The mRNA (guanine-7-)methyltransferase domain of the vaccinia virus mRNA capping enzyme. Expression in Escherichia coli and structural and kinetic comparison to the intact capping enzyme.

  1994cited by this paper
• Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides.

  1994cited by this paper
• Intrinsic RNA (guanine-7) methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit. Identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer.

  1994cited by this paper
• Characterization of the interaction of wheat germ protein synthesis initiation factor eIF-3 with mRNA oligonucleotide and cap analogues.

  1992cited by this paper
• The vaccinia virus mRNA (guanine-N7-)-methyltransferase requires both subunits of the mRNA capping enzyme for activity.

  1992cited by this paper
• A freeze-frame view of eukaryotic transcription during elongation and capping of nascent mRNA.

  1992cited by this paper
• Discrete functional stages of vaccinia virus early transcription during a single round of RNA synthesis in vitro.

  1991cited by this paper
• A comparison of the binding of methylated cap analogues to wheat germ protein synthesis initiation factors 4F and (iso)4F.

  1991cited by this paper
• The absence of a m7G cap on beta-globin mRNA and alfalfa mosaic virus RNA 4 increases the amounts of initiation factor 4F required for translation.

  1990cited by this paper
• Catalytic activity of vaccinia mRNA capping enzyme subunits coexpressed in Escherichia coli.

  1990cited by this paper
• A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay.

  1988cited by this paper
• 5'-Terminal 7-methylguanosine and mRNA function: influence of potassium concentration on translation in vitro.

  1977cited by this paper
• Relative importance of 7-methylguanosine in ribosome binding and translation of vesicular stomatitis virus mRNA in wheat germ and reticulocyte cell-free systems.

  1977cited by this paper

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  2023cites this paper
• Biochemical characterization of mRNA capping enzyme from Faustovirus

  2023influential citation
• Chemical Modifications of mRNA Ends for Therapeutic Applications

  2023cites this paper
• Applications of Phosphate Modification and Labeling to Study (m)RNA Caps

  2017cites this paper
• Plant Translation Initiation Complex eIFiso4F Directs Pokeweed Antiviral Protein to Selectively Depurinate Uncapped Tobacco Etch Virus RNA.

  2017cites this paper
• Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m(7)GpppG.

  2016cites this paper
• mRNA capping: biological functions and applications

  2016cites this paper
• A general method for rapid and cost-efficient large-scale production of 5′ capped RNA

  2016cites this paper
• Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences.

  2016cites this paper