Interaction of CbbR and RegA* Transcription Regulators with the Rhodobacter sphaeroides cbb I Promoter-Operator Region*

The form I (cbb I ) Calvin-Benson-Bassham (CBB) reductive pentose phosphate cycle operon ofRhodobacter sphaeroides is regulated by both the transcriptional activator CbbR and the RegA/PrrA (RegB/PrrB) two-component signal transduction system. DNase I footprint analyses indicated that R. sphaeroides CbbR binds to thecbbI promoter between −10 and −70 base pairs (bp) relative to the cbbI transcription start. A cosmid carrying the R. capsulatus reg locus was capable of complementing an R. sphaeroides regA-deficient mutant to phototrophic growth with restored regulated synthesis of both photopigments and ribulose-bisphosphate carboxylase/oxygenase (Rubisco). DNase I footprint analyses, using R. capsulatusRegA*, a constitutively active mutant version of RegA, detected four RegA* binding sites within the cbbI promoter. Two sites were found within a previously identifiedcbbI promoter proximal regulatory region from −61 to −110 bp. One of these proximal RegA* binding sites overlapped that of CbbR. Two sites were within a previously identified promoter distal positive regulatory region between −301 and −415 bp. Expression from promoter insertion mutants showed that the function of the promoter distal regulatory region was helical phase-dependent. These results indicated that RegA exerts its regulatory affect on cbbI expression through direct interaction with the cbbI promoter.

• Publication year

  2000

• Venue

  Journal of Biological Chemistry

• Publication date

  2000-06-23

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/JBC.M002125200PMID 10748066
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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