Lessons from Free Energy Simulations of δ-Opioid Receptor Homodimers Involving the Fourth Transmembrane Helix

Several G protein-coupled receptors (GPCRs), including opioid receptors δOR, μOR, and κOR, have been reported to form stable dimers or oligomers in lipid bilayers and cell membranes. This notion has been recently challenged by imaging data supporting a transient nature of GPCR association. Here we use umbrella sampling reconstructed free energies of δOR homodimers involving the fourth transmembrane helix to predict their association constant. The results of these simulations, combined with estimates of diffusion-limited association rates, suggest a short lifetime for δOR homodimers in the membrane, in agreement with recent trends.

• Publication year

  2010

• Venue

  Biochemistry

• Publication date

  2010-07-09

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1021/bi100686tPMID 20617813PMCID 2914489
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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