Probing of glycosidase active sites through labeling, mutagenesis and kinetic studies

No abstract is available for this paper.

• Publication year

  1995

• Venue

  Progress in Biotechnology

• Publication date

  Unknown publication date

• Fields of study

  Chemistry

• Identifiers
  DOI 10.1016/S0921-0423(06)80097-3
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Synthesis of the protein-sequencing reagent 4-(3-pyridinylmethylaminocarboxypropyl) phenyl isothiocyanate and characterization of 4-(3-pyridinylmethylaminocarboxypropyl) phenylthiohydantoins.

  1995cited by this paper
• Site-directed mutation of the putative catalytic residues of endoglucanase CenA from Cellulomonas fimi.

  1995cited by this paper
• Liquid chromatography-electrospray ionization mass spectrometry of 4-(3-pyridinylmethylaminocarboxypropyl) phenylthiohydantoins.

  1995cited by this paper
• Identification of derivatized peptides without radiolabels: tandem mass spectrometric localization of the tagged active-site nucleophiles of two cellulases and a beta-glucosidase.

  1995cited by this paper
• Transition state structures for the hydrolysis of α-D-glucopyranosyl fluoride by retaining and inverting reactions of glycosylases.

  1994cited by this paper
• Mechanisms of cellulases and xylanases: a detailed kinetic study of the exo-beta-1,4-glycanase from Cellulomonas fimi.

  1994cited by this paper
• Mutagenic analysis of a receptor contact site on interleukin-2: preparation of an IL-2 analog with increased potency.

  1994cited by this paper
• Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry.

  1994cited by this paper
• Investigation of the active site of the cyanogenic beta-D-glucosidase (linamarase) from Manihot esculenta Crantz (cassava). II. Identification of Glu-198 as an active site carboxylate group with acid catalytic function.

  1994cited by this paper
• Mutational and crystallographic analyses of the active site residues of the bacillus circulans xylanase

  1994cited by this paper
• Crystal structure of the catalytic domain of the beta-1,4-glycanase cex from Cellulomonas fimi.

  1994cited by this paper
• Identification of Glu340 as the active-site nucleophile in human glucocerebrosidase by use of electrospray tandem mass spectrometry.

  1994cited by this paper
• Mechanisms of enzymatic glycoside hydrolysis.

  1994cited by this paper
• Changing Enzymic Reaction Mechanisms by Mutagenesis: Conversion of a Retaining Glucosidase to an Inverting Enzyme

  1994cited by this paper
• Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase.

  1993cited by this paper
• N-bromoacetyl-glycopyranosylamines as affinity labels for a beta-glucosidase and a cellulase.

  1993cited by this paper
• Human acid beta-glucosidase. N-glycosylation site occupancy and the effect of glycosylation on enzymatic activity.

  1993cited by this paper
• Animal model of Gaucher's disease from targeted disruption of the mouse glucocerebrosidase gene

  1992cited by this paper
• A less costly regimen of alglucerase to treat Gaucher's disease.

  1992cited by this paper
• Mechanism of Agrobacterium beta-glucosidase: kinetic studies.

  1992cited by this paper
• Enzyme Augmentation in Moderate to Life-Threatening Gaucher Disease

  1992cited by this paper
• Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli.

  1992cited by this paper
• Inactivation of a beta-glucosidase through the accumulation of a stable 2-deoxy-2-fluoro-alpha-D-glucopyranosyl-enzyme intermediate: a detailed investigation.

  1992cited by this paper
• Mechanistic consequences of mutation of the active site nucleophile Glu 358 in Agrobacterium beta-glucosidase.

  1992cited by this paper
• Binding energy and catalysis. Fluorinated and deoxygenated glycosides as mechanistic probes of Escherichia coli (lacZ) beta-galactosidase.

  1992cited by this paper
• TESTING THE LIMITS OF PROTEIN-LIGAND BINDING DISCRIMINATION WITH TRANSITION-STATE ANALOGUE INHIBITORS

  1991cited by this paper
• Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi.

  1991cited by this paper
• General-acid and general-base catalysis of the cleavage of .alpha.-D-glucopyranosyl fluoride

  1991cited by this paper
• Glycoside hydrolases: mechanistic information from studies with reversible and irreversible inhibitors.

  1990cited by this paper
• Therapeutic response to intravenous infusions of glucocerebrosidase in a patient with Gaucher disease.

  1990cited by this paper
• Catalytic mechanism of enzymic glycosyl transfer

  1990cited by this paper
• Unequivocal demonstration of the involvement of a glutamate residue as a nucleophile in the mechanism of a retaining glycosidase

  1990cited by this paper
• Fluorinated and deoxygenated substrates as probes of transition-state structure in glycogen phosphorylase.

  1989cited by this paper
• Hazards of deducing enzyme structure-activity relationships on the basis of chemical applications of molecular biology

  1989cited by this paper
• 2-Deoxy-2-fluoro-D-glycosyl fluorides. A new class of specific mechanism-based glycosidase inhibitors.

  1988cited by this paper
• Identification of a covalent .alpha.-D-glucopyranosyl enzyme intermediate formed on a .beta.-glucosidase

  1988cited by this paper
• 2-Deoxy-2-fluoroglucosides: a novel class of mechanism-based glucosidase inhibitors

  1987cited by this paper
• Direct 1H n.m.r. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex.

  1986cited by this paper
• Molecular cloning and nucleotide sequence of human glucocerebrosidase cDNA.

  1985cited by this paper
• Reversible alkylation of a methionyl residue near the active site of -galactosidase.

  1972cited by this paper
• STEREOCHEMISTRY AND THE MECHANISM OF ENZYMATIC REACTIONS

  1953cited by this paper

• Pectinases Produced by Microorganisms: Properties and Applications

  2016cites this paper
• Plant and microbial enzymes involved in the depolymerization of (1,3)-β-d-glucans and related polysaccharides

  2009cites this paper
• Bacterial 1,3-1,4-β-glucanases: structure, function and protein engineering

  2000cites this paper
• Triggered enzymatic degradation of a water-soluble polymer solution using a novel inhibitor.

  2000cites this paper
• α-1,4-Glucan lyases producing 1,5-anhydro-d-fructose from starch and glycogen have sequence similarity to α-glucosidases

  1999cites this paper
• Neue Erkenntnisse über Hemmung, Struktur und Mechanismus konfigurationserhaltender Glycosidasen

  1999cites this paper
• The crystal structures of Sinapis alba myrosinase and a covalent glycosyl-enzyme intermediate provide insights into the substrate recognition and active-site machinery of an S-glycosidase.

  1997cites this paper
• Structure-function studies of two polysaccharide-degrading enzymes: Bacillus stearothermophilus α-amylase and Trichoderma reesei cellobiohydrolase II

  1996cites this paper