Fast nonlinear spectral microscopy of in vivo human skin

An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed.

• Publication year

  2011

• Venue

  Biomedical Optics Express

• Publication date

  2011-01-18

• Fields of study

  Materials Science, Medicine, Physics, Engineering

• Identifiers
  DOI 10.1364/BOE.2.000365PMID 21339881PMCID 3038451
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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