Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f.) Nees

BackgroundAndrographis paniculata (Burm. f.) Nees is an important medicinal plant which has enormous applications in pharmaceutical industries. Cell suspension culture of Andrographis paniculata (Burm. f.) Nees. was treated with Aspergillus niger and Penicillium expansum elicitors to enhance the synthesis of andrographolide, the bioactive constituent of A. paniculata.ResultThe elicitation treatment with fungal elicitors (A. niger and P. expansum) was observed to be most suitable for eliciting andrographolide production in the culture. The quantification of andrographolide was done using High Performance Liquid Chromatography (HPLC) technique. A. niger extract (1.5 ml with10 days treatment duration) revealed 6.94 fold increase in andrographolide content (132 μg) which was higher than the control (19 μg). P. expansum elicitor (0.6% with 8 days treatment duration) could reveal 6.23 fold enhancement in andrographolide content (81.0 μg) over control (13 μg).ConclusionThe results obtained reveal that the longer treatment duration is most favorable for the elicitation of andrographolide using both the fungal elicitors.

• Publication year

  2013

• Venue

  Botanical Studies

• Publication date

  2013-10-24

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1186/1999-3110-54-49PMID 28510886PMCID 5430361
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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