Use of viable-cell ELISA for detection of monoclonal antibodies recognizing tumor-associated antigens on bovine lymphosarcoma cells.

An ELISA, using viable bovine lymphosarcoma cells, was developed to detect tumor-associated antigens (TAA) expressed on lymphosarcoma cells from cows with enzootic bovine leukosis (EBL). Monoclonal antibodies (MAB) against TAA were used. Using viable-cell ELISA, MAB reacted with tumor cells from 9 cows with EBL, but not with peripheral blood lymphocytes from 10 clinically normal cows. Titers of MAB against tumor cells from 1 cow with EBL were 2,048 (direct immunofluorescence assay), 8,192 (flow cytometry), 2,048 (fixed-cell ELISA), and 16,384 (viable-cell ELISA). Viable-cell ELISA was the most sensitive method for detection of TAA. Reactivities of 7 MAB to tumor cells from cows with EBL were compared between viable-cell ELISA and a complement-dependent antibody cytotoxicity test. Of 5 MAB with no cytotoxic activity against tumor cells, 3 were reactive against tumor cells from the same cow, as determined by viable-cell ELISA, with titers ranging from 128 to 2,048.

• Publication year

  1987

• Venue

  American Journal of Veterinary Research

• Publication date

  1987-09-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.2460/ajvr.1987.48.09.1319PMID 2821851
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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