Enterotoxin gene profile of Staphylococcus aureus isolates recovered from bovine milk produced in central Ethiopia.

INTRODUCTION Staphylococcal food intoxication is dependent on the production of enterotoxins, the single most important virulence factors. Various studies conducted in Ethiopia have depicted the prevalence of S. aureus in bovine milk. However, there is no published data regarding the enterotoxin gene profile of S. aureus isolates in Ethiopia. The aim of this study was, therefore, to evaluate enterotoxin gene carriage profile of S. aureus isolates recovered from bovine milk samples from central Ethiopia. METHODOLOGY In this study, 109 S. aureus isolates recovered from bovine milk were analyzed for carriage of the classical enterotoxin genes. Genomic DNA extraction was performed using a commercially available kit. Two sets of multiplex polymerase chain reaction (PCR) assays were used to detect the five classical enterotoxin-coding genes and the toxic shock syndrome toxin gene. RESULTS At least one type of S. aureus enterotoxin gene (SE) was carried in 73 (66.9%) of the isolates. The most frequently encountered gene was sea (40; 36.7%) followed by seb (19; 17.4%), see (18; 16.5%), tst (16; 14.7%), sec-1 (12; 11.01%), and sed (7; 6.4%). Of the 73 S. aureus isolates harboring at least one of the enterotoxin genes, 26 (35.6%) strains harbored more than one enterotoxin gene. CONCLUSIONS More than half of the S. aureus isolates harbored at least one of the enterotoxin coding genes, indicating milk specimens contaminated by S. aureus could have a high chance of causing food intoxication.

• Publication year

  2016

• Venue

  Journal of Infection in Developing Countries

• Publication date

  2016-02-28

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology, Environmental Science

• Identifiers
  DOI 10.3855/jidc.6797PMID 26927454
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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