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Cambios morfológicos e inhibición del crecimiento de Candida albicans en presencia de una solución de sulfato de zinc

Julian Julian Gallón Nausa

Published 2015 in Unknown venue

ABSTRACT

Candida albicans is the agent responsible for the majority of the mycotic infections in the human beings5,6,7. The increase of these infections, and the appearance of resistances to diverse antifungals, makes necessary to study other substances with probable fungicidal action as it would be the case of the Sulfate of Zinc. In the present work, isolates were identified as C. albicans, in children from 4 to 12 years old, of a population of 189 children, we found a total of 13 positive samples, identified phenotypic and genotypically, proved for formation of pseudomycel and chlamidospores. These isolates were evaluated against Sulfate of Zinc’s concentrations (3%, 5% and 7%); using the method of the Minimal Inhibitory Concentration (MIC) and Kirby Bauer's method, according to the parameters of the Clinical Laboratory Standards Institute (CLSI)8. In CMI's method, with the concentrations of 3% and 5% a decrease was found for one isolate in the Colony Forming Units (CFU) related to time (24, 48 and 72 hours) compared with the stock control. As for the concentration of 7%, no growth was observed in any of three observed times. In Kirby Bauer's method, in the concentration of 3% one did not present inhibition of the growth; in the concentration of 5% and 7% one presented a halo of 16 mm and 20 mm respectively. The analysis of Microscopy and Electronic Sweep (MBE), reveals that the concentrations of Sulfate of Zinc of 3% and 5% present formation of biofilm and pseudomycel; unlike the concentration of 7 % where it was observed cellular lysis in all the fields. In conclusion the concentration of 7 % according to both secondhand technologies, supported on the observed with the MBE, is the concentration, where a major inhibitory activity is present.

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