Genomic characterisation of Almpiwar virus, Harrison Dam virus and Walkabout Creek virus; three novel rhabdoviruses from northern Australia

Abstract Rhabdoviridae represent a diverse group of viruses with the potential to cause disease in humans, animals and plants. Currently there are nine genera in the family; however a large number of rhabdoviruses remain unassigned. Here we characterise three novel rhabdoviruses genomes. Almpiwar virus (ALMV), isolated from skinks in northern Queensland, is the first completely sequenced rhabdovirus from squamates, with serological studies indicating multiple animal host species. Harrison Dam virus (HARDV) and Walkabout Creek virus (WACV) were isolated from mosquitoes in the Northern Territory and biting midges in southern Queensland respectively and their vertebrate hosts remain unknown. Serological cross-neutralisation tests with other Australian rhabdoviruses indicate that ALMV, WACV and HARDV are distinct viruses with little antigenic cross-reactivity. Next-generation sequencing revealed that all viruses encode the core proteins common to rhabdoviruses (N, P, M, G and L), plus additional ORFs between the M and G genes. HARDV also contains a small ORF between the G and L genes. Phylogenetic analysis of N and L proteins suggests that HARDV and WACV share a common lineage with the tupaviruses and Sandjimba group, whereas ALMV is a distinct and divergent virus showing no clear relationship to any rhabdovirus except the recently characterised Niahka virus (NIAV).

• Publication year

  2014

• Venue

  Virology Reports

• Publication date

  2014-09-01

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.1016/J.VIREP.2014.09.001
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Niakha virus: a novel member of the family Rhabdoviridae isolated from phlebotomine sandflies in Senegal.

  2013cited by this paper
• Human respiratory syncytial virus viroporin SH: a viral recognition pathway used by the host to signal inflammasome activation

  2012cited by this paper
• phylogenetics with

  2012cited by this paper
• A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa

  2012cited by this paper
• Ephemeroviruses: arthropod-borne rhabdoviruses of ruminants, with large, complex genomes

  2012cited by this paper
• Initial sequence characterization of the rhabdoviruses of squamate reptiles, including a novel rhabdovirus from a caiman lizard (Dracaena guianensis).

  2012cited by this paper
• The Small Hydrophobic Protein of the Human Respiratory Syncytial Virus Forms Pentameric Ion Channels*

  2012cited by this paper
• Genomic Sequences of Australian Bluetongue Virus Prototype Serotypes Reveal Global Relationships and Possible Routes of Entry into Australia

  2012cited by this paper
• SignalP 4.0: discriminating signal peptides from transmembrane regions

  2011cited by this paper
• Genetic characterization of K13965, a strain of Oak Vale virus from Western Australia.

  2011cited by this paper
• Genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional ORF that could be expressed in fish cell.

  2011cited by this paper
• Rhabdovirus accessory genes

  2011cited by this paper
• Characterization of Durham virus, a novel rhabdovirus that encodes both a C and SH protein.

  2011cited by this paper
• Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

  2011cited by this paper
• MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.

  2011cited by this paper
• Metagenomics and the molecular identification of novel viruses

  2010cited by this paper
• Ngaingan virus, a macropod-associated rhabdovirus, contains a second glycoprotein gene and seven novel open reading frames.

  2010cited by this paper
• Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF.

  2008cited by this paper
• Genomic characterisation of Wongabel virus reveals novel genes within the Rhabdoviridae.

  2008cited by this paper
• A Conserved Motif in Region V of the Large Polymerase Proteins of Nonsegmented Negative-Sense RNA Viruses That Is Essential for mRNA Capping

  2007cited by this paper
• Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments.

  2007cited by this paper
• Phylogenetic relationships of seven previously unclassified viruses within the family Rhabdoviridae using partial nucleoprotein gene sequences.

  2006cited by this paper
• Characterization of the Tupaia Rhabdovirus Genome Reveals a Long Open Reading Frame Overlapping with P and a Novel Gene Encoding a Small Hydrophobic Protein

  2005cited by this paper
• Phylogenetic relationships among rhabdoviruses inferred using the L polymerase gene.

  2005cited by this paper
• MUSCLE: multiple sequence alignment with high accuracy and high throughput.

  2004cited by this paper
• A combined transmembrane topology and signal peptide prediction method.

  2004cited by this paper
• The PredictProtein server

  2003cited by this paper
• Lyssavirus P gene characterisation provides insights into the phylogeny of the genus and identifies structural similarities and diversity within the encoded phosphoprotein.

  2002cited by this paper
• EMBOSS: the European Molecular Biology Open Software Suite.

  2000cited by this paper
• Deduced structural model for animal rhabdovirus glycoproteins.

  1999cited by this paper
• Protein identification and analysis tools in the ExPASy server.

  1999cited by this paper
• Identification of a region of the rabies virus N protein involved in direct binding to the viral RNA.

  1998cited by this paper
• A small highly basic protein is encoded in overlapping frame within the P gene of vesicular stomatitis virus

  1993cited by this paper
• Seroepidemiology of arboviruses among seabirds and island residents of the Great Barrier Reef and Coral Sea

  1991cited by this paper
• Antigenic relationships among rhabdoviruses from vertebrates and hematophagous arthropods.

  1989cited by this paper
• Identification of four conserved motifs among the RNA‐dependent polymerase encoding elements.

  1989cited by this paper
• Isolation and characterization of a tupaia rhabdovirus.

  1986cited by this paper
• Isolation of arboviruses from insects collected at Beatrice Hill, Northern Territory of Australia, 1974-1976.

  1984cited by this paper
• Antigenic relationship among rhabdoviruses infecting terrestrial vertebrates.

  1983cited by this paper
• Viruses isolated from reptiles: Identification of three new members of the familyRhabdoviridae

  1979cited by this paper
• Multiplication of viruses isolated from arthopods and vertebrates in Australia in experimentally infected mosquitoes.

  1973cited by this paper
• A survey of antibody to 10 arboviruses (Koongol group, Mapputta group and ungrouped) isolated in Queensland.

  1970cited by this paper
• Production and characterization of arbovirus antibody in mouse ascitic fluid.

  1967cited by this paper
• A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS

  1938cited by this paper

• Genomic characterization of three unclassified rhabdoviruses from mosquitoes in Malaysia and Central Africa

  2025cites this paper
• The diverse liver viromes of Australian geckos and skinks are dominated by hepaciviruses and picornaviruses and reflect host taxonomy and habitat

  2024cites this paper
• The diverse viromes of Australian lizards are shaped by host taxonomy and habitat

  2024cites this paper
• The role of sand flies as vectors of viruses other than phleboviruses.

  2023cites this paper
• Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa

  2021influential citation
• 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.

  2020cites this paper
• Virus Taxonomy The ICTV Report on Virus Classification and Taxon Nomenclature Rhabdoviridae Chapter Rhabdoviridae

  2020cites this paper
• Research Of The Vıral Hemorrhagıc Septısemıa Vırus(Vhsv) Phenotypıc Characterıstıcs Effect On Vırulence

  2019cites this paper
• Exploiting the Legacy of the Arbovirus Hunters

  2019cites this paper
• Addicted to sugar: roles of glycans in the order Mononegavirales

  2018cites this paper
• Genomic characterisation of Cuiaba and Charleville viruses: arboviruses (family Rhabdoviridae, genus Sripuvirus) infecting reptiles and amphibians

  2018cites this paper
• Genomic Characterisation of Vinegar Hill Virus, An Australian Nairovirus Isolated in 1983 from Argas Robertsi Ticks Collected from Cattle Egrets

  2017cites this paper
• Identification of very small open reading frames in the genomes of Holmes Jungle virus, Ord River virus, and Wongabel virus of the genus Hapavirus, family Rhabdoviridae

  2017cites this paper
• The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins.

  2017cites this paper
• Taxonomy of the order Mononegavirales: update 2017

  2017cites this paper
• Monitoring of Viral Induced Cell Death Using Real Time Cell Analysis

  2016cites this paper