Development of novel microsatellite markers for the spider genus Loxosceles (Sicariidae) using next-generation sequencing

Abstract We report the step-by-step process of developing de novo microsatellite (SSR) loci in two Loxosceles spider species. We used reads obtained with next-generation sequencing (Roche 454) to select hundreds of potentially-amplifiable SSRs. After testing amplification and cross-amplification, we characterized 18 SSRs, 11 of which were polymorphic in Loxosceles rufescens (Dufour 1820) and seven of which were polymorphic in L. sp. Fuerteventura - Lanzarote. This method is a relatively fast and economic procedure for the development of fast-evolving nuclear markers in spiders.

• Publication year

  2014

• Venue

  Unknown venue

• Publication date

  2014-11-13

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.1636/B13-88.1
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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