The Effects of Old Age on Hepatic Stellate Cells

Aging is associated with marked changes in the hepatic sinusoid, yet the effect of old age on hepatic stellate cells (HSC) has not been well described. Transmission electron microscopy and immunohistochemistry were used to study the effects of aging on HSC in livers from rats (3-4 mths versus 24–27 mths) and mice (2-3 mths versus 20–22 mths). Desmin-positive HSC doubled in old age in both mice and rats. Alpha-smooth muscle actin- (αSMA-) positive cells did not increase significantly and remained only a small percentage of desmin-positive cells. Electron microscopy revealed that old age is associated with HSC that have a substantial increase in the number of lipid droplets which are larger in diameter. There was also a marked increase of HSC that protruded into the sinusoidal lumen in old mice. In conclusion, old age is associated with hyperplasia of HSC that are not activated and are engorged with lipid droplets.

• Publication year

  2011

• Venue

  Current Gerontology and Geriatrics Research

• Publication date

  2011-05-18

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1155/2011/439835PMID 21687587PMCID 3114409
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• I and J

  2012cited by this paper
• Hepatic stellate cell lipid droplets: a specialized lipid droplet for retinoid storage.

  2009cited by this paper
• Sinusoidal endothelial cells prevent rat stellate cell activation and promote reversion to quiescence

  2008cited by this paper
• Old Age and the Hepatic Sinusoid

  2008influential reference
• Age-related changes in the hepatic microcirculation in mice.

  2007cited by this paper
• Basal activity of Kupffer cells increases with old age.

  2007cited by this paper
• Vitamin A-storing cells (stellate cells).

  2007cited by this paper
• Hepatic pseudocapillarization in aged mice.

  2005cited by this paper
• Sexual dimorphism in lipid metabolic phenotype associated with old age in Sprague-Dawley rats.

  2004cited by this paper
• Structure and function of hepatic stellate cells

  2004cited by this paper
• Mast Cell Density, Hepatic Stellate Cell Activation and TGF-β1 Transcripts in the Aging Sprague-Dawley Rat During Early Acute Liver Injury

  2003cited by this paper
• Mast cell density, hepatic stellate cell activation and TGF-b1 transcript in aging rat during early acute liver injury

  2003cited by this paper
• Hepatic sinusoidal pseudocapillarization with aging in the non-human primate.

  2003cited by this paper
• In vivo quantification of ageing changes in the rat liver from early juvenile to senescent life.

  2002cited by this paper
• Pseudocapillarization and associated energy limitation in the aged rat liver

  2001cited by this paper
• Comparison of the postprandial plasma vitamin A response in young and older adults.

  1998cited by this paper
• Vitamin A and E intakes and plasma concentrations of retinol, beta-carotene, and alpha-tocopherol in men and women of the Baltimore Longitudinal Study of Aging.

  1994cited by this paper
• Vitamin A and E intakes and plasma concentrations of retinol , / 3-carotene , and a-tocopherol in men and women of the Baltimore Longitudinal Study of Aging 1 ’ 2

  1994cited by this paper
• AGEING HAS NO EFFECT ON THE VOLUME DENSITY OF HEPATOCYTES, RETICULOENDOTHELIAL CELLS OR THE EXTRACELLULAR SPACE IN LIVERS OF FEMALE SPRAGUE‐DAWLEY RATS

  1992influential reference
• Phenotypic modulation of perisinusoidal cells following acute liver injury: a quantitative analysis.

  1992cited by this paper
• Comparative endotoxin‐induced hepatic injury in young and aged rats

  1990cited by this paper
• and as an in

  year unknowncited by this paper

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  2025cites this paper
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  2022influential citation
• Extended criteria grafts and emerging therapeutics strategy in liver transplantation. The unstable balance between damage and repair.

  2021cites this paper
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• Impact of Aging on Liver Cells and Liver Disease: Focus on the Biliary and Vascular Compartments

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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
• Aging and Chronic Liver Disease

  2020cites this paper
• Hallmarks of Aging in the Liver

  2019influential citation
• Aging and the Biological Response to Liver Injury

  2019cites this paper
• Liver regeneration in aged mice: new insights

  2018cites this paper
• New ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrations

  2018cites this paper
• MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage

  2018cites this paper
• Effects of aging on liver microcirculatory function and sinusoidal phenotype

  2018cites this paper
• Novel targets for delaying aging: The importance of the liver and advances in drug delivery.

  2018cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
• Long live the liver: immunohistochemical and stereological study of hepatocytes, liver sinusoidal endothelial cells, Kupffer cells and hepatic stellate cells of male and female rats throughout ageing

  2016influential citation
• Aging enhances liver fibrotic response in mice through hampering extracellular matrix remodeling

  2016cites this paper
• Aging and liver disease.

  2015cites this paper
• Liver Sinusoidal Endothelial Cells.

  2015cites this paper
• Effect of Caloric Restriction on Hepatic Sinusoidal System and Stellate Cells in Mice

  2014influential citation
• Ultrastructure of the Liver Sinusoid

  2014cites this paper
• Regression of liver fibrosis is progressive after sustained virological response to HCV therapy in patients with hepatitis C and HIV coinfection

  2013cites this paper
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  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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  2011cites this paper