Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145

Nos ultimos anos tem crescido o uso de xilanases em muitas industrias, tais como polpa e papel, alimentos e textil. Thermomyces lanuginosus IOC-4145 foi capaz de produzir um alto nivel de xilanase livre de celulase em culturas agitadas usando sabugo de milho como substrato (500 U/mL). Procedeu-se, inicialmente, a otimizacao da composicao do meio de producao em fermentacao submersa, com o intuito de alcancar uma composicao de meio de producao de baixo custo para producao da enzima. Para este proposito, utilizou-se planejamento estatistico de experimentos. O sabugo de milho revelou-se como a mais importante variavel que afeta a producao enzimatica. Adicionalmente, a influencia de varios reagentes na atividade xilanasica foi investigada no extrato bruto. Um pequeno estimulo na atividade enzimatica (5-15%) foi observado para NaCl e ureia, ambos nas concentracoes de 3 e 5 mM. Por outro lado, ditiotreitol e b-mercaptoetanol 5mM causaram um grande estimulo na atividade xilanasica (40-53%). A xilanase produzida apresentou apreciavel termoestabilidade, retendo quase 50% da atividade inicial durante 24 horas de incubacao a 50oC e, aproximadamente, 50% da atividade foram mantidos a 60oC, mesmo apos 4 horas de incubacao. A enzima tambem exibiu boa estabilidade a estocagem a -20oC na ausencia de qualquer agente estabilizante.

• Publication year

  2002

• Venue

  Brazilian Journal of Microbiology

• Publication date

  2002-12-01

• Fields of study

  Not labeled

• Identifiers
  DOI 10.1590/S1517-83822002000400011
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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