Purification and Characterization of the Recombinant Thermus sp. Strain T2 α-Galactosidase Expressed in Escherichia coli

No abstract is available for this paper.

• Publication year

  2001

• Venue

  Applied and Environmental Microbiology

• Publication date

  2001-04-01

• Fields of study

  Biology, Chemistry

• Identifiers
  DOI 10.1128/AEM.67.4.1601-1616.2001
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Thermostable alpha-galactosidase from Bacillus stearothermophilus NUB3621: cloning, sequencing and characterization.

  1999cited by this paper
• Cloning of the Gene Encoding a Novel Thermostable α-Galactosidase from Thermus brockianus ITI360

  1999cited by this paper
• Thermostable α‐galactosidase from Thermotoga neapolitana: cloning, sequencing and expression

  1998cited by this paper
• Properties of an α-galactosidase, and structure of its gene galA, within an α- and β-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima

  1998cited by this paper
• Purification, characterization, and cDNA cloning of a novel alpha-galactosidase from Mortierella vinacea.

  1997cited by this paper
• Purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068

  1997cited by this paper
• Substrate Specificities of α-Galactosidases from Yeasts

  1997cited by this paper
• An Efficient PCR Mutagenesis Strategy Without Gel Purificiation Step That Is Amenable to Automation

  1996cited by this paper
• Three α‐Galactosidase Genes of Trichoderma reesei Cloned by Expression in Yeast

  1996cited by this paper
• Purification and some properties of alpha-galactosidase from Penicillium purpurogenum.

  1995cited by this paper
• Nucleotide Sequence of α-Galactosidase cDNA from Mortierella vinacea

  1995cited by this paper
• Cloning and functional expression of a cDNA encoding coffee bean α-galactosidase

  1994cited by this paper
• Phylogeny of twenty Thermus isolates constructed from 16S rRNA gene sequence data.

  1993cited by this paper
• Induction of alpha‐galactosidase in Penicillium ochrochloron by guar (Cyamopsis tetragonobola) gum

  1993cited by this paper
• Biochemical and genetic analysis of Streptococcus mutans alpha-galactosidase.

  1991cited by this paper
• A classification of glycosyl hydrolases based on amino acid sequence similarities.

  1991cited by this paper
• Substrate Specificity of α-Galactosidase from Aspergillus niger 5–16

  1991cited by this paper
• A simple method for elucidating structures of galactomanno-oligosaccharides by sequential actions of beta-mannosidase and alpha-galactosidase.

  1990cited by this paper
• Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27

  1990cited by this paper
• Substrate Specificity of α-Galactosidase from Mortierella vinacea

  1990cited by this paper
• Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli

  1989cited by this paper
• Nucleotide sequence of the melA gene, Coding for α-galactosidase in Escherichia coli K-12

  1987cited by this paper
• Human alpha-galactosidase A: nucleotide sequence of a cDNA clone encoding the mature enzyme.

  1986cited by this paper
• Analysis of the inducible MEL1 gene of Saccharomyces carlsbergensis and its secreted product, alpha-galactosidase (melibiase).

  1985cited by this paper
• Biochemistry of the multiple forms of glycosidases in plants.

  1984cited by this paper
• DNA sequencing with chain-terminating inhibitors.

  1977cited by this paper
• Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid.

  1976cited by this paper
• Raffinose Metabolism in Escherichia coli K12

  1976cited by this paper
• Beta-galactosidase from termination and deletion mutant strains.

  1974influential reference
• β-Galactosidase from Termination and Deletion Mutant Strains

  1974cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Biochemical and genetic analysis of Streptococcus mutans a-galactosidase

  year unknowncited by this paper

• Biovalorization of cheese whey and molasses wastes to galactosidases by recombinant yeasts

  2020cites this paper
• Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides

  2019cites this paper
• Microbial production and biotechnological applications of α-galactosidase.

  2019cites this paper
• A novel α-galactosidase from the thermophilic probiotic Bacillus coagulans with remarkable protease-resistance and high hydrolytic activity

  2018cites this paper
• 16 – α-Galactosidases

  2017cites this paper
• Thermus thermophilus as source of thermozymes for biotechnological applications: homologous expression and biochemical characterization of an α-galactosidase

  2017cites this paper
• Isolation of a protease-resistant and pH-stable α-galactosidase displaying hydrolytic efficacy toward raffinose family oligosaccharides from the button mushroom Agaricus bisporus.

  2017cites this paper
• A protease-resistant α-galactosidase from Pleurotus djamor with broad pH stability and good hydrolytic activity toward raffinose family oligosaccharides.

  2017cites this paper
• Characterization of two novel heat-active α-galactosidases from thermophilic bacteria

  2016cites this paper
• Hooked on α-d-galactosidases: from biomedicine to enzymatic synthesis

  2016cites this paper
• High-level expression of a novel α-galactosidase gene from Rhizomucor miehei in Pichia pastoris and characterization of the recombinant enyzme.

  2015cites this paper
• Caracterización e aplicación biotecnolóxica da enzima α-galactosidasa de Saccharomyces cerevisiae

  2014cites this paper
• Characterization and site-directed mutagenesis of an α-galactosidase from the deep-sea bacterium Bacillus megaterium.

  2014cites this paper
• Biotechnological potential of microbial α-galactosidases

  2014cites this paper
• Partial Characterization of α-Galactosidic Activity from the Antarctic Bacterial Isolate, Paenibacillus sp. LX-20 as a Potential Feed Enzyme Source

  2012cites this paper
• Antiproliferative activity of some novel platinum complexes on C6 glioma and MCF-7 breast cancer cells

  2012cites this paper
• Lignocellulose Converting Enzymes from Thermophiles

  2011cites this paper
• Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-D-galactopyranoside derivatives.

  2011cites this paper
• Properties of a novel α-galactosidase from Streptomyces sp. S27 and its potential for soybean processing

  2010cites this paper
• Production of soya milk containing low flatulence-causing oligosaccharides in a packed bed reactor using immobilised α-galactosidase

  2010cites this paper
• Original article Production of soya milk containing low flatulence-causing oligosaccharides in a packed bed reactor using immobilised a-galactosidase

  2010cites this paper
• Aspergillus nidulansα‐galactosidase of glycoside hydrolase family 36 catalyses the formation of α‐galacto‐oligosaccharides by transglycosylation

  2010cites this paper
• The Tetramer Structure of the Glycoside Hydrolase Family 27 α-Galactosidase I from Umbelopsis vinacea

  2009cites this paper
• Cloning of a Novel Pyrethroid-Hydrolyzing Carboxylesterase Gene from Sphingobium sp. Strain JZ-1 and Characterization of the Gene Product

  2009cites this paper
• Stabilization of the quaternary structure of a hexameric alpha-galactosidase from Thermus sp. T2 by immobilization and post-immobilization techniques

  2008cites this paper
• Cloning and Functional Expression of an α-Galactosidase from Yersinia pestis biovar Microtus str. 91001

  2008cites this paper
• Purification and characterization of a novel protease-resistant α-galactosidase from Rhizopus sp. F78 ACCC 30795

  2007cites this paper
• Extremophiles, a Unique Resource of Biocatalysts for Industrial Biotechnology

  2007cites this paper
• Production of a Thermoresistant Alpha-galactosidase from Thermus sp. Strain T2 for Food Processing

  2007cites this paper
• Glyoxyl agarose as a new chromatographic matrix

  2006cites this paper
• Enhanced Production and Partial Characterization of Thermostable α-galactosidase by Thermotolerant Absidia sp.WL511 in Solid-state Fermentation using Response Surface Methodology

  2006influential citation
• Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization

  2005cites this paper
• Isolation, Purification and Characterization of Thermostable α-Galactosidase from Bacillus stearothermophilus (NCIM-5146).

  2004influential citation
• Selective and mild adsorption of large proteins on lowly activated immobilized metal ion affinity chromatography matrices. Purification of multimeric thermophilic enzymes overexpressed in Escherichia coli.

  2004cites this paper
• A Simple Strategy for the Purification of Large Thermophilic Proteins Overexpressed in Mesophilic Microorganisms: Application to Multimeric Enzymes from Thermus sp. Strain T2 Expressed in Escherichia coli

  2004cites this paper
• Crystallization and preliminary X-ray crystallographic studies of alpha-galactosidase I from Mortierella vinacea.

  2003cites this paper
• Crystal Structure of Rice α-Galactosidase Complexed with D-Galactose*

  2003cites this paper
• Crystallization and preliminary X-ray crystallographic studies of rice α-galactosidase

  2002cites this paper