Reconstitution of the Bacterial Core Light-harvesting Complexes of Rhodobacter sphaeroides and Rhodospirillum rubrum with Isolated - and -Polypeptides, Bacteriochlorophyll a, and Carotenoid (*)

Methodology has been developed to reconstitute carotenoids and bacteriochlorophyll a with isolated lightharvesting complex I (LHI) polypeptides of both Rhodobacter sphaeroides and Rhodospirillum rubrum. Reconstitution techniques first developed in this laboratory using the LHI polypeptides of R. rubrum, R. sphaeroides, and Rhodobacter capsulatus reproduced bacteriochlorophyll a spectral properties characteristic of LHI complexes lacking carotenoids. In this study, carotenoids are supplied either as organic-solvent extracts of chromatophores or as thin-layer chromatography or high performance liquid chromatography-purified species. The resulting LHI complexes exhibit carotenoid and bacteriochlorophyll a spectral properties characteristic of native LHI complexes of carotenoid-containing bacteria. Absorption and circular dichroism spectra support the attainment of a native-like carotenoid environment in the reconstituted LHI complexes. For both R. sphaeroides- and R. rubrum-reconstituted systems, fluorescence excitation spectra reveal appropriate carotenoid to bacteriochlorophyll a energy-transfer efficiencies based on comparisons with the in vivo systems. In the case of R. rubrum reconstitutions, carotenoids afford protection from photodynamic degradation. Thus, carotenoids reconstituted into LHI exhibit spectral and functional characteristics associated with native pigments. Heterologous reconstitutions demonstrate the applicability of the developed assay in dissecting the molecular environment of carotenoids in light-harvesting complexes.

• Publication year

  1995

• Venue

  Journal of Biological Chemistry

• Publication date

  1995-03-17

• Fields of study

  Biology, Chemistry, Environmental Science

• Identifiers
  DOI 10.1074/JBC.270.11.5793
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• Heterologous reconstitutions show that the assay can be used to probe the molecular environment of carotenoids in light-harvesting complexes.

  Confidence 0.90

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• Fluorescence excitation spectra indicate appropriate carotenoid-to-bacteriochlorophyll a energy transfer in the reconstituted systems, and in R. rubrum the carotenoids also protect against photodynamic degradation.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• Carotenoid-supplemented reconstituted LHI complexes display absorption and circular dichroism properties consistent with a native-like carotenoid environment.

  Confidence 0.94

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• Isolated LHI polypeptides from Rhodobacter sphaeroides and Rhodospirillum rubrum can be reconstituted in vitro with carotenoids and bacteriochlorophyll a using the developed reconstitution assay.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review

• bacteriochlorophyll a

  pigments

  The bacteriochlorophyll pigment incorporated with LHI polypeptides in the reconstitution experiments.

  Aliases: BChl a

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• carotenoids

  pigments

  Accessory pigments supplied to the LHI reconstitution mixtures as chromatophore extracts or purified species.

  Aliases: carotenoid

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• carotenoid-to-bacteriochlorophyll a energy transfer

  processes

  The transfer of excitation energy from carotenoids to bacteriochlorophyll a within LHI complexes.

  Aliases: energy-transfer efficiency, carotenoid to bacteriochlorophyll a energy transfer

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• fluorescence excitation spectra

  spectra

  Spectra used to examine how carotenoid excitation contributes to bacteriochlorophyll a emission in the reconstituted complexes.

  Aliases: excitation spectra

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• heterologous reconstitutions

  experimental configurations

  Reconstitutions assembled from LHI components and pigments drawn from different bacterial species.

  Aliases: heterologous system, cross-species reconstitution

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• light-harvesting complex i (lhi) polypeptides

  proteins

  Isolated protein subunits from bacterial core light-harvesting complex I used as the scaffold for pigment reconstitution.

  Aliases: LHI polypeptides, core light-harvesting complex I polypeptides

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• native-like carotenoid environment

  molecular environments

  The local pigment-binding context of carotenoids within LHI complexes.

  Aliases: native-like environment

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• photodynamic degradation

  phenomena

  Light-associated pigment damage assessed in the Rhodospirillum rubrum reconstitutions.

  Aliases: photodynamic damage

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• reconstitution assay

  methods

  An in vitro procedure for combining isolated LHI polypeptides with pigments.

  Aliases: reconstitution technique, reconstitution methodology, assay

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review

• Enzymatic and chemical cleavage of the core light-harvesting polypeptides of photosynthetic bacteria: determination of the minimal polypeptide size and structure required for subunit and light-harvesting complex formation.

  1995cited by this paper
• Reconstitution of a functional photosynthetic receptor complex with isolated subunits of core light-harvesting complex and reaction centers.

  1994cited by this paper
• LOCATION OF THE CAROTENOID 2Ag‐STATE AND ITS ROLE IN PHOTOSYNTHESIS

  1993cited by this paper
• Electrochromic responses of carotenoid absorbance bands in purified light-harvesting complexes from Rhodobacter capsulatus reconstituted into liposomes.

  1993cited by this paper
• CAROTENOID‐TO‐BACTERIOCHLOROPHYLL SINGLET ENERGY TRANSFER IN CAROTENOID‐INCORPORATED B850 LIGHT‐HARVESTING COMPLEXES OF Rhodobacter sphaeroides R‐26.1

  1993cited by this paper
• Multistep Electron and Energy Transfer in Artificial Photosynthesis

  1993cited by this paper
• Isolation and characterisation of the core light-harvesting complex B875 and its subunit form, B820, from Rhodocyclus gelatinosus

  1993cited by this paper
• A structural role of the carotenoid in the light-harvesting II protein of Rhodobacter capsulatus.

  1993cited by this paper
• Low-lying electronic levels of spheroidene bound to the light-harvesting (LH2) complex of Rhodobacter sphaeroides 2.4.1 as determined by fluorescence and fluorescence—excitation spectroscopy at 170 K

  1993cited by this paper
• Carotenoid singlet states and their involvement in photosynthetic light-harvesting pigments

  1992cited by this paper
• Structure, function and organization of antenna polypeptides and antenna complexes from the three families of Rhodospirillaneae.

  1992cited by this paper
• Mutants of Rhodobacter sphaeroides lacking one or more pigment‐protein complexes and complementation with reaction‐centre, LH1, and LH2 genes

  1992cited by this paper
• Spectroscopic characterization of B820 subunits from light-harvesting complex I of Rhodospirillum rubrum and Rhodobacter sphaeroides prepared with the detergent n-octyl-rac-2,3-dipropylsulfoxide

  1992cited by this paper
• Characterization of LHI- and LHI+ Rhodobacter capsulatus pufA mutants

  1992cited by this paper
• ABSORPTION SPECTRAL SHIFTS OF CAROTENOIDS RELATED TO MEDIUM POLARIZABILITY

  1991cited by this paper
• Spectroscopic characterization of the light-harvesting complex of Rhodospirillum rubrum and its structural subunit.

  1990cited by this paper
• Factors controlling the efficiency of energy transfer from carotenoids to bacteriochlorophyll in purple photosynthetic bacteria

  1990cited by this paper
• CONFIGURATIONS OF CAROTENOIDS IN THE REACTION CENTER and THE LIGHT‐HARVESTING COMPLEX OF Rhodospirillum rubrum. NATURAL SELECTION OF CAROTENOID CONFIGURATIONS BY PIGMENT PROTEIN COMPLEXES

  1990cited by this paper
• The contribution of the carotenoid to the visible circular dichroism of the light-harvesting antenna of Rhodospirillum rubrum.

  1990cited by this paper
• Supramolecular arrangement of Rhodospirillum rubrum B880 holochrome as studied by radiation inactivation and electron paramagnetic resonance.

  1990cited by this paper
• Surface-enhanced resonance raman scattering spectroscopy of bacterial photosynthetic membranes: orientation of the carotenoids of Rhodobacter sphaeroides 2.4.1.

  1990cited by this paper
• ISOLATION AND CHARACTERIZATION OF A STRUCTURAL SUBUNIT FROM THE CORE LIGHT‐HARVESTING COMPLEX OF Rhodobacter sphaeroides 2.4.1 AND puc705‐BA

  1990cited by this paper
• Probing the bacteriochlorophyll binding site by reconstitution of the light-harvesting complex of Rhodospirillum rubrum with bacteriochlorophyll a analogues.

  1990cited by this paper
• Posttranscriptional control of puc operon expression of B800-850 light-harvesting complex formation in Rhodobacter sphaeroides

  1989cited by this paper
• Changes in the native carotenoid of bacterial pigment-protein complexes

  1989cited by this paper
• Temperature dependence of the vesicle-micelle transition of egg phosphatidylcholine and octyl glucoside.

  1989cited by this paper
• Surface-enhanced resonance Raman scattering spectroscopy of bacterial photosynthetic membranes. The carotenoid of Rhodospirillum rubrum.

  1988cited by this paper
• Reconstitution of the B873 light-harvesting complex of Rhodospirillum rubrum from the separately isolated alpha- and beta-polypeptides and bacteriochlorophyll a.

  1988cited by this paper
• Roles of bacteriochlorophyll and carotenoid synthesis in formation of intracytoplasmic membrane systems and pigment-protein complexes in an aerobic photosynthetic bacterium, Erythrobacter sp. strain OCh114

  1988cited by this paper
• SPECTRA AND EXTINCTION COEFFICIENTS OF NEAR‐INFRARED ABSORPTION BANDS IN MEMBRANES OF Rhodobacter sphaeroides MUTANTS LACKING LIGHT‐HARVESTING AND REACTION CENTER COMPLEXES

  1988cited by this paper
• The light-harvesting and protective functions of carotenoids in photosynthetic membranes

  1987cited by this paper
• Isolation and characterization of a subunit form of the light-harvesting complex of Rhodospirillum rubrum.

  1987cited by this paper
• How carotenoids function in photosynthetic bacteria.

  1987cited by this paper
• Electron-spin resonance studies of carotenoids incorporated into reaction centers of Rhodobacter sphaeroides R26.1

  1986cited by this paper
• A spectral characterisation of the light-harvesting pigment-protein complexes from Rhodopseudomonas acidophila

  1986cited by this paper
• CIRCULAR DICHROISM OF LIGHT‐HARVESTING COMPLEXES FROM PURPLE PHOTOSYNTHETIC BACTERIA *

  1985cited by this paper
• Resonance Raman studies of the conformations of all-Trans carotenoids in light-harvesting systems of photosynthetic bacteria

  1985cited by this paper
• The light-harvesting polypeptides of Rhodospirillum rubrum. I. The amino-acid sequence of the second light-harvestng polypeptide B 880-beta (B 870-beta) of Rhodospirillum rubrum S 1 and the carotenoidless mutant G-9+. carotenoidless mutant G-9+.

  1984cited by this paper
• The light-harvesting polypeptides of Rhodopseudomonas sphaeroides R-26.1. I. Isolation, purification and sequence analyses.

  1984cited by this paper
• Low-temperature optical properties and pigment organization of the B875 light-harvesting bacteriochlorophyll-protein complex of purple photosynthetic bacteria

  1984cited by this paper
• On the location of the carotenoids in the light-harvesting pigment-protein complexes of the photosynthetic bacterium Rhodopseudomonas capsulata

  1983cited by this paper
• Configuration of the carotenoid in the reaction centers of photosynthetic bacteria. Comparison of the resonance Raman spectrum of the reaction center of Rhodopseudomonas sphaeroides G1C with those of cis-trans isomers of β-carotene

  1982cited by this paper
• Energy transfer in the B800-850-carotenoid light-harvesting complex of various mutants of Rhodopseudomonas sphaeroides and of Rhodopseudomonas capsulata

  1982cited by this paper
• Theoretical studies of the electrochromic response of carotenoids in photosynthetic membranes.

  1982cited by this paper
• LINEAR AND CIRCULAR DICHROISM AND FLUORESCENCE POLARIZATION OF THE B875 LIGHT‐HARVESTING BACTERIOCHLOROPHYLL‐PROTEIN COMPLEX FROM RHODOPSEUDOMONAS SPHAEROIDES

  1981cited by this paper
• Reconstitution of carotenoids into the light-harvesting pigment-protein complex from the carotenoidless mutant of Rhodopseudomonas as sphaeroides R26.

  1981cited by this paper
• Linear and circular dichroism of membranes from Rhodopseudomonas capsulata.

  1981cited by this paper
• Energy transfer between the carotenoid and the bacteriochlorophyll within the B-800-850 light-harvesting pigment-protein complex of Rhodopseudomonas sphaeroides.

  1981cited by this paper
• The complete amino acid sequence of the single light harvesting protein from chromatophores of Rhodospirillium rubrum G‐9+

  1981cited by this paper
• Binding of carotenoids on reaction centers from Rhodopseudomonas sphaeroides R 26.

  1980cited by this paper
• LIGHT ABSORPTION AND ENERGY TRANSFER IN POLYENE‐PORPHYRIN ESTERS *

  1980cited by this paper
• Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresis.

  1980cited by this paper
• Short-lived delayed luminescence of photosynthetic organisms. I. Nanosecond afterglows in purple bacteria at low redox potentials.

  1979cited by this paper
• Linear dichroism of light harvesting bacteriochlorophyll proteins from Rhodopseudomonas sphaeroides in stretched polyvinyl alcohol films.

  1979cited by this paper
• ON THE ROLE OF FORBIDDEN LOW‐LYING EXCITED STATES OF LIGHT‐HARVESTING CAROTENOIDS IN ENERGY TRANSFER IN PHOTOSYNTHESIS *

  1979cited by this paper
• Carotenoid protection against oxidation

  1979cited by this paper
• Analysis of the pigment content of an antenna pigment-protein complex from three strains of Rhodopseudomonas sphaeroides.

  1978cited by this paper
• Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Fate of "old" and "new" membrane.

  1978cited by this paper
• On the state of carotenoids bound to reaction centers of photosynthetic bacteria: a resonance Raman study.

  1978cited by this paper
• Structure and function of carotenoids in the photoreaction center from Rhodospirillum rubrum

  1977cited by this paper
• The type, amount, location, and energy transfer properties of the carotenoid in reaction centers from Rhodopseudomonas sphaeroides.

  1976cited by this paper
• The pigment complement of the photosynthetic reaction center isolated from Rhodospirillum rubrum.

  1974cited by this paper
• The state of chlorophyll and carotenoid in vivo. II. A linear dichroism study of pigment orientation in photosynthetic bacteria.

  1974cited by this paper
• Primary oxidation-reduction changes during photosynthesis in Rhodospirillum rubrum.

  1966cited by this paper
• Crystal-Structure Analysis of ~-Carotene

  1964cited by this paper
• VARIATION IN ELECTRON PARAMAGNETIC RESONANCE SIGNALS OF PHOTOSYNTHETIC SYSTEMS WTTH THE REDOX LEVEL OF THEIR ENVIRONMENT

  1963cited by this paper
• Carotenoid pigment conversion in Rhodopseudomonas spheroides.

  1962cited by this paper
• Energy transfer between carotenoids and bacteriochlorophyll in chromatophores of purple bacteria.

  1959cited by this paper
• Inhibition of Carotenoid Synthesis in Photosynthetic Bacteria: Specific Inhibition of Carotenoid Synthesis in a Photosynthetic Bacterium and its Physiological Consequences

  1958cited by this paper
• Kinetic studies of pigment synthesis by non-sulfur purple bacteria.

  1957cited by this paper
• The biology of photosynthetic bacterium which lacks colored carotenoids.

  1956cited by this paper
• Studies in carotenogenesis. 23. The nature of the carotenoids in the photosynthetic bacterium Rhodopseudomonas spheroides (athiorhodaceae).

  1956cited by this paper
• Function of Carotenoids in Photosynthesis

  1955cited by this paper
• Studies in carotenogenesis. 14. Carotenoid synthesis in the photosynthetic bacterium Rhodopseudomonas spheroides.

  1955cited by this paper
• A Theory of Sensitized Luminescence in Solids

  1953cited by this paper

• Assembly, selectivity, and compatibility of bacterial photosynthetic complexes from divergent species detected in a chimeric strain.

  2026cites this paper
• Differential stability of bacterial photosynthetic apparatus of Rhodobacter alkalitolerans strain JA916T under alkaline and light environment

  2024cites this paper
• Cryo-EM structure of the Rhodospirillum rubrum RC–LH1 complex at 2.5 Å

  2021cites this paper
• Excitonic coupling effect on the circular dichroism spectrum of sodium-pumping rhodopsin KR2.

  2020cites this paper
• Tuning the Photophysical Features of Self-Assembling Photoactive Polypeptides for Light-Harvesting

  2019influential citation
• Biochemical and Spectroscopic Characterizations of a Hybrid Light-Harvesting Reaction Center Core Complex.

  2018cites this paper
• Structure and Function of the Reaction Centre – Light Harvesting 1 Core Complexes from Purple Photosynthetic Bacteria

  2017cites this paper
• Probing structure–function relationships in early events in photosynthesis using a chimeric photocomplex

  2017cites this paper
• Strategies to enhance the excitation energy-transfer efficiency in a light-harvesting system using the intra-molecular charge transfer character of carotenoids.

  2017cites this paper
• Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red-shifting

  2017cites this paper
• Light harvesting in phototrophic bacteria: structure and function.

  2017cites this paper
• Formation of a subunit form of the core light-harvesting complex from sulfur purple bacteria Ectothiorhodospira haloalkaliphila with different carotenoid composition

  2016cites this paper
• PucC and LhaA direct efficient assembly of the light‐harvesting complexes in Rhodobacter sphaeroides

  2015cites this paper
• Natural and artificial light-harvesting systems utilizing the functions of carotenoids

  2015cites this paper
• Adaptation of the Photosynthetic Unit of Purple Bacteria to Changes of Light Illumination Intensities

  2015cites this paper
• Structure of the LH1–RC complex from Thermochromatium tepidum at 3.0 Å

  2014cites this paper
• Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy*

  2014cites this paper
• Controlling Structural and Functional Features of Photosynthetic Antenna

  2012cites this paper
• Antioxidant effects of carotenoids in a model pigment-protein complex.

  2012cites this paper
• Reconstitution and Organization of Photosynthetic Antenna Protein Complex Bearing Functional Hydrophilic Domains

  2011cites this paper
• Carotenoids are essential for normal levels of dimerisation of the RC-LH1-PufX core complex of Rhodobacter sphaeroides: characterisation of R-26 as a crtB (phytoene synthase) mutant.

  2011cites this paper
• Removal and Reconstitution of the Carotenoid Antenna of Xanthorhodopsin

  2010cites this paper
• Structural Forming of Photosynthetic Polypeptide Supramolecule Complexes and Functional Analysis of Carotenoids in These Complexes

  2010cites this paper
• Structure-Function Relationships in Bacterial Light-Harvesting Complexes Investigated by Reconstitution Techniques

  2009cites this paper
• Design and Assembly of Functional Light-Harvesting Complexes

  2009influential citation
• Reconstitution of Gloeobacter violaceus rhodopsin with a light-harvesting carotenoid antenna.

  2009cites this paper
• Ultrafast quantum dynamics in photosynthesis

  2008cites this paper
• Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum.

  2008cites this paper
• Probing the Carotenoid in Its Binding Site in a Reconstituted LH1 Complex from the Photosynthetic Bacterium Rhodospirillum rubrum with Electroabsorption Spectroscopy

  2008cites this paper
• Single-molecule spectroscopic characterization of light-harvesting 2 complexes reconstituted into model membranes.

  2007cites this paper
• Induced chirality of the light-harvesting carotenoid salinixanthin and its interaction with the retinal of xanthorhodopsin.

  2006cites this paper
• Assembly of Model Bacteriochlorophyll Proteins in the Native Lipid Environment

  2006cites this paper
• The solution structure of the PufX polypeptide from Rhodobacter sphaeroides

  2006cites this paper
• The PufX protein of Rhodobacter capsulatus affects the properties of bacteriochlorophyll a and carotenoid pigments of light-harvesting complex 1.

  2005cites this paper
• Trapping of an Assembly Intermediate of Photosynthetic LH1 Antenna beyond B820 Subunit

  2005cites this paper
• Circular dichroism and absorption spectroscopic data reveal binding of the natural cis-carotenoid bixin to human α1-acid glycoprotein

  2005cites this paper
• Thermodynamics of Light-induced and Thermal Degradation of Bacteriochlorins in Reaction Center Protein of Photosynthetic Bacteria

  2005cites this paper
• Analysis of Binding Interaction between the Natural Apocarotenoid Bixin and Human Serum Albumin by Circular Dichroism and Fluorescence Spectroscopy

  2005cites this paper
• Effects of Carotenoid Inhibition on the Photosynthetic RC–LH1 Complex in Purple Sulphur Bacterium Thiorhodospira sibirica

  2005influential citation
• Interactions stabilizing the structure of the core light-harvesting complex (LH1) of photosynthetic bacteria and its subunit (B820).

  2004cites this paper
• Hydrogen Bonding in a Model Bacteriochlorophyll-binding Site Drives Assembly of Light Harvesting Complex*

  2004cites this paper
• Interaction of bacteriochlorophyll with the LH1 and PufX polypeptides of photosynthetic bacteria: use of chemically synthesized analogs and covalently attached fluorescent probes

  2004cites this paper
• Identification of intramembrane hydrogen bonding between 13(1) keto group of bacteriochlorophyll and serine residue alpha27 in the LH2 light-harvesting complex.

  2003cites this paper
• On‐line monitoring of Phaffia rhodozyma fed‐batch process with in situ dispersive raman spectroscopy

  2003cites this paper
• Further insight into the molecular basis of carotenoid–albumin interactions: circular dichroism and electronic absorption study on different crocetin–albumin complexes

  2002cites this paper
• The solution structure of Rhodobacter sphaeroides LH1beta reveals two helical domains separated by a more flexible region: structural consequences for the LH1 complex.

  2000cites this paper
• Liquid chromatography of natural pigments.

  2000cites this paper
• Carotenoids and the Assembly of Light-harvesting Complexes

  1999cites this paper
• Minimal requirements for in vitro reconstitution of the structural subunit of light-harvesting complexes of photosynthetic bacteria

  1999cites this paper
• Differential carotenoid composition of the B875 and B800-850 photosynthetic antenna complexes in Rhodobacter sphaeroides 2.4.1: involvement of spheroidene and spheroidenone in adaptation to changes in light intensity and oxygen availability

  1996cites this paper
• Correlation of HPLC and AOAC Methods to Assess the All‐trans‐Lutein Content in Marigold Flowers

  1996cites this paper
• Purple bacterial antenna complexes.

  1996cites this paper
• Light-harvesting mechanisms in purple photosynthetic bacteria.

  1995cites this paper
• Structure-Function Relationships in Core Light-Harvesting Complexes (LHI) As Determined by Characterization of the Structural Subunit and by Reconstitution Experiments

  1995cites this paper
• Antioxidant Effects of Carotenoids in a Model Pigment–protein Complex*

  year unknowncites this paper