Sorghum husk biomass as a potential substrate for production of cellulolytic and xylanolytic enzymes by Nocardiopsis sp. KNU

Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme. Cellulase produced by Nocardiopsis sp. KNU utilizing sorghum husk as a substrate was found to retain its stability in various surfactants up to 90%. The produced enzyme was further tested for saccharification of mild alkali pretreated rice husk. The changes in morphology and functional group were analyzed using scanning electron microscopy and Fourier transform infrared spectroscopy. Enzymatic saccharification confirmed the hydrolytic potential of crude cellulase. The hydrolysate products were analyzed by high-performance thin layer chromatography.

• Publication year

  2017

• Venue

  3 Biotech

• Publication date

  2017-06-29

• Fields of study

  Biology, Materials Science, Chemistry, Environmental Science, Medicine

• Identifiers
  DOI 10.1007/s13205-017-0800-zPMID 28660456PMCID 5489444
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2015influential reference
• Modification in the properties of paper by using cellulase-free xylanase produced from alkalophilic Cellulosimicrobium cellulans CKMX1 in biobleaching of wheat straw pulp.

  2015influential reference
• Production and characterization of cellulolytic enzymes by isolated Klebsiella sp. PRW-1 using agricultural waste biomass

  2014cited by this paper
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  2014cited by this paper
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  2013cited by this paper
• Biodiesel and biohydrogen production from cotton-seed cake in a biorefinery concept.

  2013cited by this paper
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  2013cited by this paper
• Alkaline protease production, extraction and characterization from alkaliphilic Bacillus licheniformis KBDL4: a Lonar soda lake isolate.

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• Optimization of xylanase production by Streptomyces sp. P12-137 using response surface methodology and central composite design

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  2011cited by this paper
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  2010cited by this paper
• Deconstructing recalcitrant Miscanthus with alkaline peroxide and electrolyzed water.

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  2009cited by this paper
• Sugarcane bagasse hemicellulose hydrolysate for ethanol production by acid recovery process

  2008cited by this paper
• Genomics of cellulosic biofuels

  2008cited by this paper
• Physicochemical Characterization of Rice Straw Pretreated with Sodium Hydroxide in the Solid State for Enhancing Biogas Production

  2008cited by this paper
• Streptomyces drozdowiczii cellulase production using agro-industrial by-products and its potential use in the detergent and textile industries

  2005cited by this paper
• Statistical optimization of a thermostable and neutral glucoamylase production by a thermophilic mold Thermomucor indicae-seudaticae in solid-state fermentation

  2004cited by this paper
• Isolation and characterization of cellulose from sugarcane bagasse

  2004cited by this paper
• Characterization of Chitinase Genes from an Alkaliphilic Actinomycete, Nocardiopsis prasina OPC-131

  2003influential reference
• Hydrolysis of lignocellulosic materials for ethanol production: a review.

  2002cited by this paper
• Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose

  2002cited by this paper
• Microbial Cellulose Utilization: Fundamentals and Biotechnology

  2002influential reference
• Production and characterization of a thermostable alpha-amylase from Nocardiopsis sp. endophyte of yam bean.

  2001cited by this paper
• Molecular cloning and analysis of a pleiotropic regulatory gene locus from the nystatin producer Streptomyces noursei ATCC11455.

  1999cited by this paper
• Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake

  1998cited by this paper
• Effect of surfactants on cellulase production by Nectria catalinensis

  1996influential reference
• The characteristics of a new non-spore-forming cellulolytic mesophilic anaerobe strain CM126 isolated from municipal sewage sludge.

  1991cited by this paper
• Measurement of cellulase activities

  1987cited by this paper
• Surfactants as Stimulants of Enzyme Production by Microorganisms

  1969influential reference
• Seed germination studies. I. Purification and properties of an alpha-amylase from the cotyledons of germinating peas.

  1966cited by this paper
• Relation of certain infrared bands to cellulose crystallinity and crystal latticed type. Part I. Spectra of lattice types I, II, III and of amorphous cellulose

  1964cited by this paper
• Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

  1959cited by this paper

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  2023cites this paper
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  2022cites this paper
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• Cellulase and xylanase synergism in industrial biotechnology

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