An E3 ubiquitin ligase TRIM9 is involved in WSSV infection via interaction with &bgr;‐TrCP

&NA; The TRIpartite Motif (TRIM) proteins are known to play key roles in cell differentiation, apoptosis, development, autophagy and innate immunity. In the present study, a TRIM9 homolog (named LvTRIM9) was identified from the transcriptome of the Pacific whiteleg shrimp Litopenaeus vannamei. The deduced amino acid sequence of LvTRIM9 possessed typical features of TRIMs, consisting of a RING domain, two B‐boxes, a coiled‐coil domain, a FN3 domain, and a SPRY domain. The transcript of LvTRIM9 was detected in most tissues of the shrimp. Its expression level was obviously up‐regulated at 3, 12 and 24 h post white spot syndrome virus (WSSV) infection. Knockdown of LvTRIM9 gene expression by double‐strand RNA mediated interference could lead to a decrease of virus copy number in WSSV‐infected shrimp. Yeast two‐hybrid analysis showed that LvTRIM9 could directly interact with beta‐transducin repeat‐containing protein of shrimp (Lv&bgr;‐TrCP), an inhibitor of NF‐&kgr;B pathway. Meanwhile, knockdown of LvTRIM9 could also up‐regulate the expression levels of LvRelish and downstream production of antimicrobial peptides in the intestine of shrimp. These data indicated that WSSV might hijack the LvTRIM9 for its propagation through inhibition of NF‐&kgr;B pathway and downstream antimicrobial peptides production via interaction of LvTRIM9 with Lv&bgr;‐TrCP in shrimp. The study improved our understanding about the impact of E3 ubiquitin ligases on the innate immune signaling pathway of shrimp and its role during WSSV infection.

• Publication year

  2019

• Venue

  Developmental and Comparative Immunology

• Publication date

  2019-08-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.dci.2019.03.014PMID 30910419
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mutual Stabilization between TRIM9 Short Isoform and MKK6 Potentiates p38 Signaling to Synergistically Suppress Glioblastoma Progression.

  2018cited by this paper
• Structural determinants of TRIM protein function.

  2017cited by this paper
• TRIM9-dependent ubiquitination of DCC constrains kinase signaling, exocytosis, and axon branching

  2017cited by this paper
• TRIM Family Proteins: Roles in Autophagy, Immunity, and Carcinogenesis.

  2017cited by this paper
• Identification and function analysis of a novel vascular endothelial growth factor, LvVEGF3, in the Pacific whiteleg shrimp Litopenaeus vannamei.

  2016cited by this paper
• Trim9 Deletion Alters the Morphogenesis of Developing and Adult-Born Hippocampal Neurons and Impairs Spatial Learning and Memory

  2016cited by this paper
• In-depth comparative transcriptome analysis of intestines of red swamp crayfish, Procambarus clarkii, infected with WSSV

  2016cited by this paper
• Trim9 mediates macrophage chemotaxis in a RING-dependent manner

  2016cited by this paper
• TRIM9 short isoform preferentially promotes DNA and RNA virus-induced production of type I interferon by recruiting GSK3β to TBK1

  2016influential reference
• The first molluscan TRIM9 is involved in the negative regulation of NF-κB activity in the Hong Kong oyster, Crassostrea hongkongensis.

  2016cited by this paper
• The E3 Ubiquitin Ligase TRIM9 Is a Filopodia Off Switch Required for Netrin-Dependent Axon Guidance.

  2015cited by this paper
• Promoter methylation of TRIM9 as a marker for detection of circulating tumor DNA in breast cancer patients

  2015cited by this paper
• TRIMmunity: the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity.

  2014cited by this paper
• The ubiquitin system in immune regulation.

  2014cited by this paper
• Negative regulation of NF-κB activity by brain-specific TRIpartite Motif protein 9

  2014cited by this paper
• The Ubiquitination Machinery of the Ubiquitin System

  2014cited by this paper
• Interferon-stimulated genes: a complex web of host defenses.

  2014cited by this paper
• InTRIMsic immunity: Positive and negative regulation of immune signaling by tripartite motif proteins

  2014cited by this paper
• RNA-Seq reveals the dynamic and diverse features of digestive enzymes during early development of Pacific white shrimp Litopenaeus vannamei.

  2014cited by this paper
• Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection

  2013cited by this paper
• SUMO-Conjugating Enzyme E2 UBC9 Mediates Viral Immediate-Early Protein SUMOylation in Crayfish To Facilitate Reproduction of White Spot Syndrome Virus

  2012cited by this paper
• Expression Profiling of WSSV ORF 199 and Shrimp Ubiquitin Conjugating Enzyme in WSSV Infected Penaeus monodon

  2012cited by this paper
• Enzyme E2 from Chinese White Shrimp Inhibits Replication of White Spot Syndrome Virus and Ubiquitinates Its RING Domain Proteins

  2011cited by this paper
• Tripartite-motif proteins and innate immune regulation.

  2011cited by this paper
• Interferon-stimulated genes and their antiviral effector functions

  2011cited by this paper
• TRIM9, a novel brain-specific E3 ubiquitin ligase, is repressed in the brain of Parkinson's disease and dementia with Lewy bodies.

  2010cited by this paper
• Identification and functional study of a shrimp Relish homologue.

  2009cited by this paper
• Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function

  2007cited by this paper
• RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis

  2005cited by this paper
• Comparative WSSV infection routes in the shrimp genera Marsupenaeus and Palaemon.

  2005cited by this paper
• TRIM9 is specifically expressed in the embryonic and adult nervous system.

  2002cited by this paper
• Ubiquitin‐mediated proteolysis: biological regulation via destruction

  2000cited by this paper

• Comparative transcriptome profiles across distinct developmental stages in mud crab Scylla paramamosain embryos

  2026cites this paper
• Identification of candidate genes associated with AHPND resistance based on genome-wide association study in Pacific white shrimp Litopenaeus vannamei.

  2025cites this paper
• Integrated pharmacoanalysis, bioinformatics analysis, and experimental validation to identify the ingredients and mechanisms of Xiao-Luo-Wan in uterine fibroids treatment

  2025cites this paper
• Ubiquitination of Hemocyanin Mediated by a Mitochondrial E3 Ubiquitin Ligase Regulates Immune Response in Penaeus vannamei.

  2024cites this paper
• Vectorial dynamics underpinning current and future tick-borne virus emergence in Europe

  2024cites this paper
• Molecular characterization and functional analysis of TRIM37 from black tiger shrimp (Penaeus monodon).

  2023cites this paper
• TRIM55 promotes noncanonical NF-κB signaling and B cell–mediated immune responses by coordinating p100 ubiquitination and processing

  2023cites this paper
• Evolution and expression of the duck TRIM gene repertoire

  2023cites this paper
• Cellular responses in crustaceans under white spot syndrome virus infection.

  2023cites this paper
• The role of pattern recognition receptors in crustacean innate immunity

  2023cites this paper
• Genome-wide identification and analysis of HECT E3 ubiquitin ligase gene family in Ruditapes philippinarum and their involvement in the response to heat stress and Vibrio anguillarum infection.

  2022cites this paper
• A Novel TRIM9 Protein Promotes NF-κB Activation Through Interacting With LvIMD in Shrimp During WSSV Infection

  2022cites this paper
• NF-κB and its crosstalk with endoplasmic reticulum stress in atherosclerosis

  2022cites this paper
• Eight TRIM32 isoforms from oriental river prawn Macrobrachium nipponense are involved in innate immunity during white spot syndrome virus infection.

  2022cites this paper
• TRIM Proteins in Inflammation: from Expression to Emerging Regulatory Mechanisms

  2021cites this paper
• Litopenaeus vannamei peroxiredoxin 2-like is involved in WSSV infection by interaction with wsv089 and VP26.

  2021cites this paper
• A tripartite motif protein (CgTRIM1) involved in CgIFNLP mediated antiviral immunity in the Pacific oyster Crassostrea gigas.

  2021cites this paper
• Ubiquitination as an Important Host-Immune Response Strategy in Penaeid Shrimp: Inferences From Other Species

  2021cites this paper
• Identification of a Shrimp E3 Ubiquitin Ligase TRIM50-Like Involved in Restricting White Spot Syndrome Virus Proliferation by Its Mediated Autophagy and Ubiquitination

  2021cites this paper
• TRIM9 is involved in facilitating Vibrio parahaemolyticus infection by inhibition of relish pathway in Penaeus monodon.

  2021cites this paper
• Trim23 promotes WSSV replication though negative regulation of antimicrobial peptides expression in Macrobrachium nipponense.

  2020cites this paper
• A Litopenaeus vannamei TRIM32 gene is involved in oxidative stress response and innate immunity.

  2020cites this paper
• Targeting NF-κB pathway for the therapy of diseases: mechanism and clinical study

  2020cites this paper
• Functional roles of E3 ubiquitin ligases in gastric cancer

  2020cites this paper
• The Two NF-κB Pathways Regulating Bacterial and WSSV Infection of Shrimp

  2019cites this paper
• To TRIM or not to TRIM: the balance of host-virus interactions mediated by the ubiquitin system.

  2019cites this paper