Rapid screening and characterization of triterpene saponins in Acanthopanax senticosus leaves via untargeted MSAll and SWATH techniques on a quadrupole time of flight mass spectrometry

HighlightsDPIF of key substructures was applied to identify Acanthopanax TSs using MSAll and SWATH.Thirty‐three compounds were reported as potentially new compounds in genus Acanthopanax.A malonyl‐saponin was the first report in genus Acanthopanax. Abstract This paper focused on untargeted MSAll, also called MSE, and sequential window acquisition of all theoretical fragment‐ion spectra (SWATH) fragmentations for comprehensive structural characterization of triterpene saponins (TSs) in leaves of Acanthopanax senticosus through ultra‐high performance liquid chromatography electrospray quadrupole time‐of‐flight mass spectrometry (UPLC‐ESI‐QTOF). [M+H]+, [M + NH4]+ and [M + Na]+ precursor ions and the corresponding fragment ions were collected simultaneously in energy‐resolved MSAll. SWATH fragmentation was applied as a comparable and complementary method for resolving co‐eluting species. A workflow based on MSAll and SWATH fragmentations was constructed for comprehensive structural characterization and rapid discovery of TSs in leaves of A. senticosus. As a result, 89 TSs, along with 14 sapogenins, were unambiguously characterized or tentatively identified. Of these, 33 compounds were characterized as potentially new compounds, including the first report of malonyl‐saponin in genus Acanthopanax. This study aimed to systematically analyze TSs in leaves of A. senticosus, and the results are significant for the utilization of A. senticosus leaves.

• Publication year

  2019

• Venue

  Journal of Pharmaceutical and Biomedical Analysis

• Publication date

  2019-06-05

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.jpba.2019.02.032PMID 30909056
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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