Chromatin plates in the interphase nucleus

Early results showed the emanation of chromatin fibers from mitotic chromosomes and nuclei swollen with water. In contrast, under metaphase ionic conditions, it was found that chromatin from mitotic chromosomes is planar and forms multilayered plates. Here, we show that in buffers containing interphase cation concentrations, the chromatin emanated from disrupted nuclei also has a planar morphology. Furthermore, the chromatin fragments produced by micrococcal nuclease digestion of nuclei form the typical beads‐on‐a‐string fibers in the absence of cations, but they self‐assemble into plate‐like structures in buffers containing magnesium. The plates from interphase nuclei do not form the thick multilayered structures observed in metaphase chromosomes, suggesting that they are more exposed to the medium to facilitate DNA replication and gene expression.

• Publication year

  2019

• Venue

  FEBS Letters

• Publication date

  2019-04-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/1873-3468.13370PMID 30908620
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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