Stereoselective C3‐substituent modification and substrate channeling by oxidoreductase BchC in bacteriochlorophyll a biosynthesis

We report the in vitro activity of recombinant BchC oxidoreductase involved in bacteriochlorophyll a biosynthesis. BchC of Rhodobacter capsulatus preferentially oxidizes 31R‐3‐(1‐hydroxyethyl)‐chlorophyllide a and 31R‐3‐(1‐hydroxyethyl)‐bacteriochlorophyllide a in the presence of NAD+ to 3‐acetyl‐chlorophyllide a and bacteriochlorophyllide a, respectively, leaving the unreacted 31S‐epimers. In the reverse reaction, BchC with NADH predominately produces 31R‐epimeric alcohols from the 3‐acetyl‐(bacterio)chlorins. BchC of Chlorobaculum tepidum demonstrates the same 31R‐selectivity, suggesting that utilization of 31R‐epimers in BchC‐catalyzed reductions may be conserved across different phyla of photosynthetic bacteria. Additionally, the presence of BchC accelerates the 3‐vinyl hydration by BchF hydratase of Chlorobaculum tepidum during conversion of chlorophyllide a to 3‐acetyl‐chlorophyllide a through 3‐(1‐hydroxyethyl)‐chlorophyllide a, indicating that these enzymes work cooperatively to promote efficient bacteriochlorophyll a biosynthesis.

• Publication year

  2019

• Venue

  FEBS Letters

• Publication date

  2019-04-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1002/1873-3468.13372PMID 30908616
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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