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A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells.

Adiki Raja Sekhar,Bhagaban Mallik,Vimlesh Kumar,J. Sankar

Published 2019 in Organic and biomolecular chemistry

ABSTRACT

The endoplasmic reticulum (ER) constitutes about half of the total membrane of a eukaryotic cell, and defects in the ER have been shown to be linked with a variety of diseases. To investigate these underlying mechanisms in detail, the specific labelling of the ER for high-resolution long-term live-imaging can serve as an important tool. Here, we report the identification of a stimulated emission depletion (STED) microscopy-compatible BODIPY derivative (NH2-BODIPY) to selectively image the ER. In contrast to the conventional ER-Tracker™ dye, NH2-BODIPY selectively labels the ER at a much lower concentration with no detectable cytotoxicity. Conventional imaging agents are often unstable under the intense light field used for STED microscopy, but NH2-BODIPY is stable due to its robust structure and therefore it has the potential to be widely exploited for ER imaging.

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