Ectopic Expression of OsDREB1G, a Member of the OsDREB1 Subfamily, Confers Cold Stress Tolerance in Rice

Plants adapt to adverse environmental conditions through physiological responses, such as induction of the abscisic acid signaling pathway, stomatal regulation, and root elongation. Altered gene expression is a major molecular response to adverse environmental conditions in plants. Several transcription factors function as master switches to induce the expression of stress-tolerance genes. To find out a master regulator for the cold stress tolerance in rice, we focused on functionally identifying DREB subfamily which plays important roles in cold stress tolerance of plants. Here, we characterized OsDREB1G (LOC_Os02g45450), a functionally unidentified member of the DREB1 subgroup. OsDREB1G is specifically induced under cold stress conditions among several abiotic stresses examined. This gene is dominantly expressed in leaf sheath, blade, node, and root. Transgenic rice overexpressing this gene exhibited strong cold tolerance and growth retardation, like transgenic rice overexpressing other OsDREB1 genes. However, unlike these rice lines, transgenic rice overexpressing OsDREB1G did not exhibit significant increases in drought or salt tolerance. Cold-responsive genes were highly induced in transgenic rice overexpressing DREB1G compared to wild type. In addition, OsDREB1G overexpression directly induced the expression of a reporter gene fused to the promoters of cold-induced genes in rice protoplasts. Therefore, OsDREB1G is a typical CBF/DREB1 transcription factor that specifically functions in the cold stress response. Therefore, OsDREB1G could be useful for developing transgenic rice with enhanced cold-stress tolerance.

• Publication year

  2019

• Venue

  Frontiers in Plant Science

• Publication date

  2019-03-28

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fpls.2019.00297PMID 30984209PMCID 6447655
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular characterisation of a DREB gene from Sophora moorcroftiana, an endemic species of plateau

  2017cited by this paper
• MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability.

  2017cited by this paper
• The rice transcription factors OsICE confer enhanced cold tolerance in transgenic Arabidopsis

  2017cited by this paper
• Insight into the AP2/ERF transcription factor superfamily in sesame and expression profiling of DREB subfamily under drought stress

  2016cited by this paper
• Cloning and characterization of an ABA-independent DREB transcription factor gene, HcDREB2, in Hemarthria compressa

  2016cited by this paper
• Insight into the AP 2 / ERF transcription factor superfamily in sesame and expression profiling of DREB subfamily under drought stress

  2016cited by this paper
• Ectopic expression of a hot pepper bZIP-like transcription factor in potato enhances drought tolerance without decreasing tuber yield

  2015cited by this paper
• Functional characterization and reconstitution of ABA signaling components using transient gene expression in rice protoplasts

  2015cited by this paper
• OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis.

  2015cited by this paper
• Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants.

  2015cited by this paper
• Analyzing the Expression Profile of AREB/ABF and DREB/CBF Genes under Drought and Salinity Stresses in Grape (Vitis vinifera L.)

  2015cited by this paper
• Genome-Wide Analysis of the AP2/ERF Transcription Factors Family and the Expression Patterns of DREB Genes in Moso Bamboo (Phyllostachys edulis)

  2015cited by this paper
• 骨髄異形成症候群のgenome-wide analysis

  2013cited by this paper
• Colinearity and Similar Expression Pattern of Rice DREB1s Reveal Their Functional Conservation in the Cold-Responsive Pathway

  2012cited by this paper
• Articles : Optimization of Agrobacterium-mediated Transformation in Japonica-type Rice Oryza sativa L. cv. Dongjin for high Efficiency

  2012cited by this paper
• A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth.

  2012cited by this paper
• AP2/ERF Transcription Factor in Rice: Genome-Wide Canvas and Syntenic Relationships between Monocots and Eudicots

  2012cited by this paper
• Gene structures, classification and expression models of the AP2/EREBP transcription factor family in rice.

  2011cited by this paper
• Transcription factors as tools to engineer enhanced drought stress tolerance in plants

  2011cited by this paper
• Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network

  2011cited by this paper
• Four divergent Arabidopsis ethylene‐responsive element‐binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference

  2009cited by this paper
• The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

  2009cited by this paper
• Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis.

  2009influential reference
• Over-expression of OsDREB genes lead to enhanced drought tolerance in rice

  2008cited by this paper
• Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice

  2008cited by this paper
• Gene networks involved in drought stress response and tolerance.

  2006cited by this paper
• Role of DREB transcription factors in abiotic and biotic stress tolerance in plants

  2006cited by this paper
• Genome-Wide Analysis of the ERF Gene Family in Arabidopsis and Rice[W]

  2006cited by this paper
• Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice.

  2006cited by this paper
• Structural, Functional, and Phylogenetic Characterization of a Large CBF Gene Family in Barley

  2005cited by this paper
• Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters.

  2005cited by this paper
• Effect of early cold stress on the maturation of rice anthers

  2004cited by this paper
• Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance

  2003cited by this paper
• OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression.

  2003cited by this paper
• Monitoring Expression Profiles of Rice Genes under Cold, Drought, and High-Salinity Stresses and Abscisic Acid Application Using cDNA Microarray and RNA Gel-Blot Analyses1[w]

  2003cited by this paper
• Transcription Factor CBF4 Is a Regulator of Drought Adaptation in Arabidopsis1

  2002cited by this paper
• DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression.

  2002cited by this paper
• Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation.

  2000cited by this paper
• Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor.

  1999cited by this paper
• Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor

  1999cited by this paper
• Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, Respectively, in Arabidopsis

  1998cited by this paper
• An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression.

  1998cited by this paper
• Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance.

  1998influential reference
• Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit.

  1997cited by this paper

• BdDREB2A is Involved in Responses to Heat and Drought Stress.

  2026cites this paper
• The transcription factor OsERF74 positively regulates drought resistance by modulating abscisic acid catabolism in rice.

  2026cites this paper
• Genome-wide Analysis of Rice OsDREB1 Genes Under Heat Stress Reveals Functional Specialization of OsDREB1F for Thermotolerance in Arabidopsis

  2026cites this paper
• AlphaFold 3-Assisted Deciphering of the DNA Recognition by DREB1 Transcription Factors in Rice

  2025cites this paper
• Innovation in drought-resilient agriculture: The rise of aerobic rice system

  2025cites this paper
• Genome-wide identification of DREB1 transcription factors in Erianthus fulvus and the functional role of EfDREB1C in regulating cold tolerance of transgenic Arabidopsis and sugarcane.

  2025cites this paper
• OsSARD1 Orchestrates the Ca2+/OsCaM1‐Guided Transcriptional Regulation of OsDREB1A/1B/1H Regulons Under Chilling in Rice

  2025cites this paper
• Cytokinin elevation caused by high light intensity contributes substantially to the increase of thermotolerance of rice plants

  2025cites this paper
• Melatonin regulates proline metabolism by modulating CmDREB1A/E to enhance cold tolerance in cantaloupe fruit.

  2025cites this paper
• Monoculm1 confers cold tolerance at the seedling stage in rice.

  2025cites this paper
• Brassinosteroid-signaling kinase 4 activates mitogen-activated protein kinase 4 to enhance cold stress tolerance in maize.

  2025cites this paper
• Temporal regulation of gene expression during auxin-triggered crown root formation in barley: an integrated approach

  2025cites this paper
• An A-6 subgroup member of DREB gene family positively regulates cold stress tolerance by modulating an antioxidant defense system in transgenic potato

  2025cites this paper
• Overexpression of OsPIN5b Alters Plant Architecture and Impairs Cold Tolerance in Rice (Oryza sativa L.)

  2025cites this paper
• Multi-Omics Approaches Against Abiotic and Biotic Stress—A Review

  2025cites this paper
• Strategies for plant-microbe symbiosis: Mycorrhizal fungi and helper bacteria to improve cold tolerance in rice.

  2025cites this paper
• Ethylene antagonizes ABA and inhibits stomatal closure and chilling tolerance in rice.

  2025cites this paper
• Transcription Factors in Plant Biotic and Abiotic Stress Responses: Potentials and Prospects in Sugarcane

  2025cites this paper
• Rice Responses to Abiotic Stress: Key Proteins and Molecular Mechanisms

  2025cites this paper
• Genome-Wide Association Analysis of Soybean Regeneration-Related Traits and Functional Exploration of Candidate Genes

  2025cites this paper
• Mycorrhizal helper bacteria further promote mycorrhizal fungi to improve cold tolerance in rice seedlings: evidence from oxidative stress, osmoregulation, photosynthesis, and related genes in rice

  2025cites this paper
• OsEIN2-OsEIL1/2 pathway negatively regulates chilling tolerance by attenuating OsICE1 function in rice.

  2024cites this paper
• Development of an infiltration-based RNA preservation method for cryogen-free storage of leaves for gene expression analyses in field-grown plants

  2024cites this paper
• Uncovering Key Transcription Factors Driving Chilling Stress Tolerance in Rice Germination

  2024cites this paper
• Deciphering the genomic regions associated with seedling cold tolerance traits in rice (Oryza sativa L.)

  2024cites this paper
• The role of priming and memory in rice environmental stress adaptation: Current knowledge and perspectives.

  2024cites this paper
• Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3

  2024cites this paper
• OsGADD45a1: a multifaceted regulator of rice architecture, grain yield, and blast resistance

  2024cites this paper
• Ectopic overexpression of ShCBF1 and SlCBF1 in tomato suggests an alternative view of fruit responses to chilling stress postharvest

  2024cites this paper
• Phytochrome interacting factor ZmPIF6 simultaneously enhances chilling tolerance and grain size in rice.

  2024cites this paper
• The Molecular Mechanism of Cold-Stress Tolerance: Cold Responsive Genes and Their Mechanisms in Rice (Oryza sativa L.)

  2024cites this paper
• Overexpression of MsDREB1C Modulates Growth and Improves Forage Quality in Tetraploid Alfalfa (Medicago sativa L.)

  2024cites this paper
• Precise colocalization of sorghum’s major chilling tolerance locus with Tannin1 due to tight linkage drag rather than antagonistic pleiotropy

  2024cites this paper
• Hormonal responses of rice to organ-targeted cold stress

  2024cites this paper
• Understanding the cross-talk of major abiotic-stress-responsive genes in rice: A computational biology approach

  2023cites this paper
• ‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (Triticum aestivum)

  2023cites this paper
• Novel inducible promoter DREB1G cloned from date palm exhibits high fold expression over AtRD29 to drought and salinity stress

  2023cites this paper
• Transcription factors MhDREB2A/MhZAT10 play a role in drought and cold stress response crosstalk in apple

  2023cites this paper
• De Novo Transcriptome Assembly and Comparative Analysis of Differentially Expressed Genes Involved in Cold Acclimation and Freezing Tolerance of the Arctic Moss Aulacomnium turgidum (Wahlenb.) Schwaegr

  2023cites this paper
• Introducing sorghum DREB2 gene in maize (Zea mays L.) to improve drought and salinity tolerance

  2023cites this paper
• Genome-Wide Identification, Evolution, and Expression Analyses of AP2/ERF Family Transcription Factors in Erianthus fulvus

  2023cites this paper
• Overexpression of CdtCIPK21 from triploid bermudagrass reduces salt and drought tolerance but increases chilling tolerance in transgenic rice.

  2023cites this paper
• Biocuration of a Transcription Factors Network Involved in Submergence Tolerance during Seed Germination and Coleoptile Elongation in Rice (Oryza sativa)

  2023cites this paper
• Genome-wide identification and expression analysis of the Pisum sativum (L.) APETALA2/ethylene-responsive factor (AP2/ERF) gene family reveals functions in drought and cold stresses

  2023cites this paper
• The DREB transcription factor, a biomacromolecule, responds to abiotic stress by regulating the expression of stress-related genes.

  2023cites this paper
• Overexpression of Rice Histone H1 Gene Reduces Tolerance to Cold and Heat Stress

  2023cites this paper
• A homolog of AtCBFs, SmDREB A1-4, positively regulates salt stress tolerance in Arabidopsis thaliana and Salix matsudana.

  2023cites this paper
• A comprehensive investigation of the regulatory roles of OsERF096, an AP2/ERF transcription factor, in rice cold stress response

  2023cites this paper
• Advances and opportunities in unraveling cold‐tolerance mechanisms in the world's primary staple food crops

  2023cites this paper
• Role of the Rice BAHD Acyltransferase Gene OsAt10 in Plant Cold Stress Tolerance

  2022cites this paper
• Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

  2022cites this paper
• Quantitative trait loci, candidate genes, and breeding lines to improve salt tolerance at the flowering and seedling stages in rice

  2022cites this paper
• Research Progress of AP2/ERF Transcription Factor Family in Important Crops

  2022cites this paper
• Functional Characterization of Tea Plant (Camellia sinensis L.) CsCBF2 Gene Involved in Multiple Abiotic Stress Response in Tobacco (Nicotiana tabacum L.)

  2022cites this paper
• Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response

  2022cites this paper
• ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

  2022cites this paper
• Sugarcane ScDREB2B-1 Confers Drought Stress Tolerance in Transgenic Nicotiana benthamiana by Regulating the ABA Signal, ROS Level and Stress-Related Gene Expression

  2022cites this paper
• CRISPR/Cas9-mediated mutation in auxin efflux carrier OsPIN9 confers chilling tolerance by modulating reactive oxygen species homeostasis in rice

  2022cites this paper
• Overexpression of Asr6, Abscisic acid stress-ripening protein, enhances drought tolerance and modulates gene expression in rice (Oryza sativa L.)

  2022cites this paper
• Overexpression of AmCBF1 enhances drought and cold stress tolerance, and improves photosynthesis in transgenic cotton

  2022cites this paper
• Dehydration-Responsive Element Binding Protein 1C, 1E, and 1G Promote Stress Tolerance to Chilling, Heat, Drought, and Salt in Rice

  2022cites this paper
• Genetic Improvement of Wheat for Drought Tolerance: Progress, Challenges and Opportunities

  2022cites this paper
• Evaluation of the Morpho-Physiological, Biochemical and Molecular Responses of Contrasting Medicago truncatula Lines under Water Deficit Stress

  2021cites this paper
• Toward Integrated Multi-Omics Intervention: Rice Trait Improvement and Stress Management

  2021cites this paper
• Decreased night temperature affects development and grain yield only in cold-susceptible rice (Oryza sativa) plants

  2021cites this paper
• Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response

  2021cites this paper
• Integrated global analysis reveals a vitamin E-vitamin K1 sub-network, downstream of COLD1, underlying rice chilling tolerance divergence.

  2021cites this paper
• Genome-wide identification and expression profiling of DREB genes in Saccharum spontaneum

  2021cites this paper
• Estimation of DREB Gene Expression in Wheat Genotypes (Triticum aestivum L.) Introduced to Anbar Governorate Under Water Stress

  2021cites this paper
• Harnessing the potential of plant transcription factors in developing climate resilient crops to improve global food security: Current and future perspectives

  2021cites this paper
• Genome-Wide Identification and Characterization of AP2/ERF Transcription Factor Family Genes in Oil Palm under Abiotic Stress Conditions

  2021cites this paper
• Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

  2020cites this paper
• Genetically Modified Rice Stacked with Antioxidants for Nutrient Enhancement and Stress Tolerance

  2020cites this paper
• Ectopic expression of BrALDH7B2 gene encoding an antiquitin from Brassica rapa confers tolerance to abiotic stresses and improves photosynthetic performance under salt stress in tobacco

  2020cites this paper
• The dehydrin gene of the Arctic plant Cerastium arcticum, CaDHN, increases tolerance to multiple stresses in Arabidopsis thaliana

  2020cites this paper
• Harnessing the Potential of Plant Transcription Factors in Developing Climate-Smart Crops: Future Prospects, Challenges, and Opportunities

  2020cites this paper
• The role of key transcription factors for cold tolerance in plants

  2020cites this paper
• DREB Duo Defines Distinct Drought and Cold Response Pathways[OPEN]

  2019cites this paper
• An Update on Molecular Strategies of Transgenic Rice Tolerance to Abiotic Stresses

  2019cites this paper
• A novel method for preserving leaves to facilitate gene expression analyses in eld-grown plants

  year unknowncites this paper