A PDZ domain‐based assay for measuring HIV protease activity: Assay design considerations

No abstract is available for this paper.

• Publication year

  2003

• Venue

  Protein Science

• Publication date

  2003-03-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1110/ps.0235603PMID 12592016PMCID PMC2312448
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A Comparison of ALPHAScreen, TR-FRET, and TRF as Assay Methods for FXR Nuclear Receptors

  2002cited by this paper
• PDZ Domains: Structural Modules for Protein Complex Assembly*

  2002cited by this paper
• PDZ domains: troubles in classification

  2002cited by this paper
• Structural Determinants of the Na+/H+Exchanger Regulatory Factor Interaction with the β2Adrenergic and Platelet-derived Growth Factor Receptors*

  2002cited by this paper
• A PDZ domain-based detection system for enzymatic assays.

  2002cited by this paper
• Computer-aided design of a PDZ domain to recognize new target sequences

  2002cited by this paper
• Classification of PDZ domains

  2001cited by this paper
• Energetic determinants of internal motif recognition by PDZ domains.

  2001cited by this paper
• Crystal structure of the PDZ1 domain of human Na(+)/H(+) exchanger regulatory factor provides insights into the mechanism of carboxyl-terminal leucine recognition by class I PDZ domains.

  2001influential reference
• Distinct Binding Specificity of the Multiple PDZ Domains of INADL, a Human Protein with Homology to INAD fromDrosophila melanogaster *

  2001cited by this paper
• Regulation of cystic fibrosis transmembrane conductance regulator single-channel gating by bivalent PDZ-domain-mediated interaction.

  2001influential reference
• Structural Basis of the Na+/H+ Exchanger Regulatory Factor PDZ1 Interaction with the Carboxyl-terminal Region of the Cystic Fibrosis Transmembrane Conductance Regulator*

  2001influential reference
• Platelet-Derived Growth Factor Receptor Association with Na+/H+ Exchanger Regulatory Factor Potentiates Receptor Activity

  2000cited by this paper
• Single-amino acid substitutions alter the specificity and affinity of PDZ domains for their ligands.

  2000cited by this paper
• Substrate recognition through a PDZ domain in tail-specific protease.

  2000cited by this paper
• Analysis of PDZ Domain-Ligand Interactions Using Carboxyl-terminal Phage Display*

  2000cited by this paper
• Accessory protein facilitated CFTR-CFTR interaction, a molecular mechanism to potentiate the chloride channel activity.

  2000cited by this paper
• Presenilin 1 is linked with g -secretase activity in the detergent solubilized state

  2000cited by this paper
• Presenilin 1 is linked with gamma-secretase activity in the detergent solubilized state.

  2000cited by this paper
• Rapid identification of substrates for novel proteases using a combinatorial peptide library.

  2000cited by this paper
• HIV Protease as a Target for the Design of Antiviral Agents for AIDS

  1999cited by this paper
• Non-active Site Changes Elicit Broad-based Cross-resistance of the HIV-1 Protease to Inhibitors*

  1999cited by this paper
• The Carboxyl Terminus of B Class Ephrins Constitutes a PDZ Domain Binding Motif*

  1999cited by this paper
• Ligand-receptor binding measured by laser-scanning imaging.

  1999cited by this paper
• Mutagenesis and selection of PDZ domains that bind new protein targets

  1999cited by this paper
• PDZ domains: fundamental building blocks in the organization of protein complexes at the plasma membrane.

  1999cited by this paper
• Peptide binding consensus of the NHE‐RF‐PDZ1 domain matches the C‐terminal sequence of cystic fibrosis transmembrane conductance regulator (CFTR)

  1998influential reference
• Crystal structure of the hCASK PDZ domain reveals the structural basis of class II PDZ domain target recognition

  1998cited by this paper
• A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins.

  1998influential reference
• CRIPT, a novel postsynaptic protein that binds to the third PDZ domain of PSD-95/SAP90.

  1998influential reference
• Two distantly positioned PDZ domains mediate multivalent INAD–phospholipase C interactions essential for G protein‐coupled signaling

  1998cited by this paper
• An Apical PDZ Protein Anchors the Cystic Fibrosis Transmembrane Conductance Regulator to the Cytoskeleton*

  1998influential reference
• A fluorescence polarization based Src-SH2 binding assay.

  1997influential reference
• Recognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ Domains

  1997cited by this paper
• PDZ domain of neuronal nitric oxide synthase recognizes novel C-terminal peptide sequences

  1997cited by this paper
• Measurement of the binding of tyrosyl phosphopeptides to SH2 domains: a reappraisal.

  1995cited by this paper
• Luminescent oxygen channeling immunoassay: measurement of particle binding kinetics by chemiluminescence.

  1994cited by this paper
• Kinetics of p56lck and p60src Src homology 2 domain binding to tyrosine-phosphorylated peptides determined by a competition assay or surface plasmon resonance.

  1993cited by this paper
• Different requirements for productive interaction between the active site of HIV-1 proteinase and substrates containing -hydrophobic*hydrophobic- or -aromatic*pro- cleavage sites.

  1992cited by this paper
• A cumulative specificity model for proteases from human immunodeficiency virus types 1 and 2, inferred from statistical analysis of an extended substrate data base.

  1991cited by this paper
• Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

  1988cited by this paper
• Time-resolved detection of energy transfer: theory and application to immunoassays.

  1988cited by this paper
• Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA

  year unknowncited by this paper

• Design and verification of halogen-bonding system at the complex interface of human fertilization-related MUP PDZ5 domain with CAMK's C-terminal peptide

  2017cites this paper
• HIV-Screening Strategies for the Discovery of Novel HIV-Inhibitors

  2011cites this paper
• Engineering a protein for peptide detection and allosteric activation

  2010cites this paper
• The Use of AlphaScreen Technology in HTS: Current Status

  2008cites this paper
• High-Throughput Screening and Insect Genomics for New Insecticide Leads

  2007cites this paper
• Directed evolution of PDZ variants to generate high-affinity detection reagents.

  2005cites this paper
• Progress in Lanthanides as Luminescent Probes

  2005cites this paper
• Mechanism of SMRT corepressor recruitment by the BCL6 BTB domain.

  2003cites this paper