ImuB and ImuC contribute to UV‐induced mutagenesis as part of the SOS regulon in Pseudomonas aeruginosa

DNA damage‐induced mutagenesis is a process governed by the SOS system that requires the activity of specialized DNA polymerases. These polymerases, which are devoid of proof‐reading activity, serve to increase the probability of survival under stressful conditions in exchange for an error‐prone DNA synthesis. As an opportunistic pathogen of humans, Pseudomonas aeruginosa employs adaptive responses that originally evolved for survival in many diverse and often stressful environmental conditions, where the action of error‐prone DNA polymerases may be crucial. In this study, we have investigated the role of the polymerases ImuB and ImuC in P. aeruginosa DNA‐damage induced mutagenesis. UV irradiation of imuB‐ and imuC‐deletion mutants showed that both genes contribute to UV‐induced mutagenesis in this bacterium. Furthermore, we confirmed that UV treatment significantly increase the expression levels of the imuB and imuC genes and that they are co‐transcribed as a single transcriptional unit under the control of LexA as part of the SOS regulon in P. aeruginosa. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.

• Publication year

  2019

• Venue

  Environmental and Molecular Mutagenesis

• Publication date

  2019-08-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1002/em.22290PMID 30921487
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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