Simultaneous detection of nicotinamide adenine nucleotides and adenylate pool to quantify redox and energy states in mAb-producing CHO cells by capillary electrophoresis

No abstract is available for this paper.

• Publication year

  2019

• Venue

  Analytical and Bioanalytical Chemistry

• Publication date

  2019-03-28

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1007/s00216-019-01747-9PMID 30923861
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1985cited by this paper
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