In vitro regeneration from different ages of petioles of physic nut (Jatropha curcas L.)

Jatropha curcas L. is an important non-edible oil yielding plant growing in wasteland and dry lands. The experiment was conducted to study the plant regeneration of J. curcas from different ages of petioles. Petioles explant grown on Murashige and Skoog’s (MS) medium supplemented with 1 mg/l α-naphthalene acetic acid and 0.2 mg/l kinetin showed the highest frequency of callus induction and the same medium was found to be best suited for callus growth in 1st petiole. Percent of somatic embryo formation was higher (35.16%) from the 1st petiole explant on 0.5 mg/l thidiazuron with 0.4 mg/l gibberellic acid. MS medium supplemented with 1 mg/l N 6 -benzylaminopurine, 0.5 mg/l indole-3-acetic acid, 0.25 mg/l kinetin recorded the higher somatic embryo germination percentage (66.85%). MS basal medium supplemented with 0.5 mg/l thidiazuron, 0.1 mg/l N 6 -benzylaminopurine, 0.4 mg/l gibberellic acid was found to be best medium for shoot elongation. Among the auxins tested, the higher frequency of root formation was observed in MS medium supplemented with 0.3 mg/l α-naphthalene acetic acid compared to indole-3-butyric acid and indole-3-acetic acid. The survival rate of in vitro rooted plantlets was dependent on the type of explant source and it varies from 47 to 60%. Keywords: Euphorbiaceae, petiole, callus, indirect organogenesis, biodiesel, plant growth regulator African Journal of Biotechnology , Vol. 13(2), pp. 265-273, 8 January, 2014

• Publication year

  2014

• Venue

  African Journal of Biotechnology

• Publication date

  2014-01-08

• Fields of study

  Agricultural and Food Sciences, Biology

• Identifiers
  DOI 10.5897/AJB2013.12995
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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