Molecular characterization and expression analysis of a hepcidin gene from rice field eel ( Monopterus albus )

amino acid sequences were presented. In the 949 bp-long genomic sequence, two introns and three exons were identified. The full-length cDNA encodes a prepropeptide of 90 amino acid residues. RTPCR analysis suggested that hepcidin transcripts are highly abundant in the liver and kidney, less abundant in the heart, skin, brain, blood cells, intestine, spleen and stomach and undetectable in muscle. After challenged with Aeromonas hydrophila infection or iron-dextran stimulation, the hepcidin transcript levels were analyzed by RT-PCR. The results revealed that the expression of hepcidin dramatically increased at 24 h post-infection of the pathogen injection. Moreover, hepcidin mRNAs in the liver, intestine and brain were 2.4, 1.5 and 2-fold increase, respectively, compared with the control animals after 5 days in iron-dextran injected RFEs.

• Publication year

  2011

• Venue

  African Journal of Biotechnology

• Publication date

  2011-08-03

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.5897/AJB10.745
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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