Rapid detection and quantification of viable potato cyst nematodes using qPCR in combination with propidium monoazide

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Plant Pathology

• Publication date

  2014-10-01

• Fields of study

  Biology, Agricultural and Food Sciences, Environmental Science

• Identifiers
  DOI 10.1111/PPA.12193
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular Typing of Cyst‐Forming Nematodes Globodera pallida and G. rostochiensis, Using Real‐Time PCR and Evaluation of Five Methods for Template Preparation

  2013cited by this paper
• Top 10 plant-parasitic nematodes in molecular plant pathology.

  2013cited by this paper
• Improving efficiency of viability-PCR for selective detection of live cells.

  2013cited by this paper
• Detection of viable potato cyst nematodes (Globodera rostochiensis and G. pallida) by reverse transcription PCR

  2012cited by this paper
• Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification.

  2012cited by this paper
• A rapid, sensitive, and cost-efficient assay to estimate viability of potato cyst nematodes.

  2012cited by this paper
• Multiplex Real-Time PCR Assays for the Identification of the Potato Cyst and Tobacco Cyst Nematodes.

  2010cited by this paper
• Cryptosporidium Propidium Monoazide-PCR, a Molecular Biology-Based Technique for Genotyping of Viable Cryptosporidium Oocysts

  2009cited by this paper
• Effect of the fungus Pochonia chlamydosporia and fosthiazate on the multiplication rate of potato cyst nematodes (Globodera pallida and G. rostochiensis) in potato crops grown under UK field conditions

  2008cited by this paper
• TaqMan REAL-Time PCR-based approach for differentiation between Globodera rostochiensis (golden nematode) and Globodera artemisiae species

  2008cited by this paper
• Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA).

  2008cited by this paper
• Morphological and Molecular Identification of Globodera pallida Associated with Potato in Idaho.

  2007cited by this paper
• Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells.

  2006cited by this paper
• Quantification of the slug parasitic nematode Phasmarhabditis hermaphrodita from soil samples using real time qPCR.

  2006cited by this paper
• Quantitative detection of the potato cyst nematode, Globodera pallida, and the beet cyst nematode, Heterodera schachtii, using Real-Time PCR with SYBR green I dye.

  2005cited by this paper
• The effect of mycorrhization of potato roots on the hatching chemicals active towards the potato cyst nematodes, Globodera pallida and G. rostochiensis

  2004cited by this paper
• Evaluation of PCR, IEF and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales.

  2001cited by this paper
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001cited by this paper
• Effects of hatching factors on potato cyst nematode hatch and in-egg mortality in soil and in vitro

  2001cited by this paper
• Effects of the biological nematicide, DiTera , on hatching of Globodera rostochiensis and G. pallida

  2000cited by this paper
• Identification of populations of potato cyst nematodes from Russia using protein electrophoresis, rDNA-RFLPs and RAPDs.

  1999cited by this paper
• Identification of potato cyst nematode species Globodera rostochiensis and G. pallida by PCR using specific primer combinations.

  1999cited by this paper
• Detection of hatching inhibitors and hatching factor stimulants for golden potato cyst nematode, Globodera rostochiensis, in potato root leachate

  1998cited by this paper
• Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR)

  1997cited by this paper
• Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

  1990cited by this paper
• Morphology and Identification of Cyst Nematodes

  1986cited by this paper
• The adenosine triphosphate content of cysts of Globodera pallida and G. rostochiensis as a possible quantitative estimate of field populations

  1977cited by this paper
• The Use of Meldola Blue and Nile Blue a, for Distinguishing Dead From Living Nematodes

  1974cited by this paper
• Methods for the Recovery and Counting of Cysts of Heterodera schachtii from Soil

  1940cited by this paper

• Revisiting steroidal glycoalkaloids as hatching stimulants for Globodera rostochiensis and Globodera pallida

  2025cites this paper
• The use of X-ray computed tomography for advanced detection of Globodera pallida

  2025cites this paper
• Combining Masculinizing Resistance, Rotation, and Biocontrol to Achieve Durable Suppression of the Potato Pale Cyst Nematode: A Model

  2024influential citation
• Potato Cyst Nematodes (PCN), Globodera Rostochiensis and G. Pallida as a New Challenging Problem of Potato Production in Africa

  2024cites this paper
• New Approaches to Plant Pathogen Detection and Disease Diagnosis.

  2024cites this paper
• Development of a simple method for simultaneous detection and differentiation of Globodera pallida and G. rostochiensis

  2022cites this paper
• Ascorbate Oxidase Induces Systemic Resistance in Sugar Beet Against Cyst Nematode Heterodera schachtii

  2020cites this paper
• Potato Cyst Nematodes: Geographical Distribution, Phylogenetic Relationships and Integrated Pest Management Outcomes in Portugal

  2020cites this paper
• Comparison of promidium monoazide and Reagent D for discrimination of viable Aphelenchoides besseyi using real-time PCR

  2020cites this paper
• Applied problems of detection and identification of golden potato nematode

  2020cites this paper
• Evaluation of Fluorescent Stains for Viability Assessment of the Potato Cyst Nematodes Globodera pallida and G. ellingtonae

  2019cites this paper
• Chapter 3 Molecular Diagnostic Tools for Nematodes

  2019cites this paper
• Cereal Cyst Nematodes: A Complex and Destructive Group of Heterodera Species.

  2017cites this paper
• A qRT-PCR method to evaluate viability of potato cyst nematode (Globodera spp.)

  2017cites this paper
• Rapid and simple detection of two potato cyst nematode species by real-time multiplex PCR using preserved microarray-based test systems

  2017cites this paper
• Molecular Diagnostic Tools for Nematodes

  2017cites this paper
• Rapid detection and quantification of viable Pseudomonas syringae pv. lachrymans cells in contaminated cucumber seeds using propidium monoazide and a real-time PCR assay

  2016cites this paper
• From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area

  2016cites this paper
• Use of ethidium monoazide and propidium monoazide to determine viral infectivity upon inactivation by heat, UV- exposure and chlorine.

  2015cites this paper
• Optimizing Trehalose-Based Quantification of Live Eggs in Potato Cyst Nematodes (Globodera rostochiensis and G. pallida).

  2015cites this paper
• Development of qPCR assays for quantitative detection of Heterodera avenae and H. latipons

  2015cites this paper
• Nested-quantitative PCR approach with improved sensitivity for the detection of low titer levels of Candidatus Liberibacter asiaticus in the Asian citrus psyllid, Diaphorina citri Kuwayama.

  2014cites this paper