Targeted mutation breeding of horticultural plants

Mutation breeding can be enhanced by genetic selection for novel alleles. Through targeted mutation breeding, genotypes with induced or natural mutations in candidate genes are identified for cultivar development. For most horticultural plants, targeted mutation breeding may be a more economically feasible approach to trait development than through transgenic technology. Substantial progress has been made in applying targeted mutation breeding to horticulture and this review summarizes recently published work in this area. To date, at least 16 horticultural crops have been screened for natural or induced allelic diversity in over 100 candidate genes. This approach has resulted in traits of commercial use, such as longer shelf-life (tomato, melon), improved starch quality (potato), and virus-resistance (peppers, tomato). Advances in genome sequencing and genetic screening will facilitate the development of cultivars with value-added traits derived from candidate gene polymorphisms.

• Publication year

  2012

• Venue

  Emirates Journal of Food and Agriculture

• Publication date

  2012-01-05

• Fields of study

  Agricultural and Food Sciences, Biology

• Identifiers
  DOI 10.9755/EJFA.V24I1.10596
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• A survey of natural and ethyl methane sulfonate-induced variations of eIF4E using high-resolution melting analysis in Capsicum

  2012cited by this paper
• Induced point mutations in the phytoene synthase 1 gene cause differences in carotenoid content during tomato fruit ripening

  2011cited by this paper
• Tomato TILLING Technology: Development of a Reverse Genetics Tool for the Efficient Isolation of Mutants from Micro-Tom Mutant Libraries

  2011cited by this paper
• Mutation scanning of peach floral genes

  2011cited by this paper
• TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.)

  2011cited by this paper
• Statistical Mutation Calling from Sequenced Overlapping DNA Pools in TILLING Experiments

  2011cited by this paper
• Model SNP development for complex genomes based on hexaploid oat using high-throughput 454 sequencing technology

  2011cited by this paper
• Genome-wide SNP discovery in tetraploid alfalfa using 454 sequencing and high resolution melting analysis

  2011cited by this paper
• Discovery of Rare Mutations in Populations: TILLING by Sequencing1[C][W][OA]

  2011cited by this paper
• Reverse-genetics by TILLING expands through the plant kingdom

  2011cited by this paper
• Expression level polymorphisms: heritable traits shaping natural variation.

  2011cited by this paper
• Single nucleotide polymorphism genotyping by heteroduplex analysis in sunflower (Helianthus annuus L.)

  2011cited by this paper
• sunTILL: a TILLING resource for gene function analysis in sunflower

  2011cited by this paper
• Towards a TILLING platform for functional genomics in Piel de Sapo melons

  2011cited by this paper
• Engineering Melon Plants with Improved Fruit Shelf Life Using the TILLING Approach

  2010cited by this paper
• Genomics and Bioinformatics Resources for Crop Improvement

  2010cited by this paper
• Discovery of nucleotide polymorphisms in the Musa gene pool by Ecotilling

  2010cited by this paper
• EcoTILLING in Capsicum species: searching for new virus resistances

  2010influential reference
• The flowering gene SINGLE FLOWER TRUSS drives heterosis for yield in tomato

  2010cited by this paper
• A new mutant genetic resource for tomato crop improvement by TILLING technology

  2010cited by this paper
• Heritable targeted mutagenesis in maize using a designed endonuclease.

  2010cited by this paper
• Synonymous codon usage influences the local protein structure observed

  2010cited by this paper
• An Induced Mutation in Tomato eIF4E Leads to Immunity to Two Potyviruses

  2010influential reference
• iTILLING: A Personalized Approach to the Identification of Induced Mutations in Arabidopsis1[C][OA]

  2010cited by this paper
• High-resolution DNA melting analysis in clinical research and diagnostics

  2010cited by this paper
• Nontransgenic Genome Modification in Plant Cells1[W][OA]

  2010cited by this paper
• Insights from the comparison of plant genome sequences.

  2010cited by this paper
• A cost-effective high-resolution melting approach using the EvaGreen dye for DNA polymorphism detection and genotyping in plants.

  2010cited by this paper
• Reverse genetics techniques: engineering loss and gain of gene function in plants.

  2010cited by this paper
• Quick-multiplex-consensus (QMC)-PCR followed by high-resolution melting: a simple and robust method for mutation detection in formalin-fixed paraffin-embedded tissue

  2010cited by this paper
• Induced Mutagenesis in Plants Using Physical and Chemical Agents

  2010cited by this paper
• Plant Cell Culture: Essential Methods

  2010cited by this paper
• Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases

  2010cited by this paper
• Maternal Plasma DNA Sequencing Reveals the Genome-Wide Genetic and Mutational Profile of the Fetus

  2010cited by this paper
• Mutation scanning and genotyping by high-resolution DNA melting analysis in olive germplasm.

  2009cited by this paper
• A Modified TILLING Method for Wheat Breeding

  2009influential reference
• Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing

  2009influential reference
• Association Studies Identify Natural Variation at PHYC Linked to Flowering Time and Morphological Variation in Pearl Millet

  2009cited by this paper
• Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1

  2009influential reference
• Optimizing TILLING and Ecotilling techniques for potato (Solanum tuberosum L)

  2009cited by this paper
• Validation of TILLING in the evaluation of inbred progenies of irradiated cassava (Manihot esculenta Crantz)

  2009cited by this paper
• New sources of soybean seed meal and oil composition traits identified through TILLING

  2009cited by this paper
• CEL I Nuclease Digestion for SNP Discovery and Marker Development in Common Bean ( Phaseolus vulgaris L.)

  2009cited by this paper
• Mapping SNP-anchored genes using high-resolution melting analysis in almond

  2009cited by this paper
• Enzyme Leads to Andromonoecy in Melons A Conserved Mutation in an Ethylene Biosynthesis

  2009cited by this paper
• Molecular evolution of the E8 promoter in tomato and some of its relative wild species

  2009cited by this paper
• Mutation discovery for crop improvement.

  2009cited by this paper
• Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato

  2009cited by this paper
• Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

  2009cited by this paper
• Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance

  2009cited by this paper
• High-Throughput Detection of Induced Mutations and Natural Variation Using KeyPoint™ Technology

  2009cited by this paper
• Forward and reverse genetics of rapid-cycling Brassica oleracea

  2009cited by this paper
• High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley

  2009cited by this paper
• MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice

  2008cited by this paper
• Reducing the content of nornicotine in tobacco via targeted mutation breeding

  2008influential reference
• Naturally occurring broad-spectrum powdery mildew resistance in a Central American tomato accession is caused by loss of mlo function.

  2008cited by this paper
• Precision breeding for novel starch variants in potato.

  2008cited by this paper
• Application of TILLING and EcoTILLING as Reverse Genetic Approaches to Elucidate the Function of Genes in Plants and Animals

  2008cited by this paper
• UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

  2008cited by this paper
• Synonymous mutations and ribosome stalling can lead to altered folding pathways and distinct minima.

  2008cited by this paper
• A Conserved Mutation in an Ethylene Biosynthesis Enzyme Leads to Andromonoecy in Melons

  2008cited by this paper
• Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea.

  2007cited by this paper
• Genomics-Assisted Crop Improvement

  2007cited by this paper
• EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

  2007cited by this paper
• Tilling and Ecotilling for Crop Improvement

  2007cited by this paper
• Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize

  2007cited by this paper
• EcoTILLING for the identification of allelic variation in the powdery mildew resistance genes mlo and Mla of barley

  2006cited by this paper
• Harvesting the potential of induced biological diversity.

  2006cited by this paper
• TILLING: practical single-nucleotide mutation discovery.

  2006cited by this paper
• GENETIC ALTERATIONS ASSOCIATED WITH THE ORIGINS OF FRUIT CULTURE

  2004cited by this paper
• MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5′ region

  2004cited by this paper
• Global impact of mutation-derived varieties

  2004cited by this paper
• Horticultural biotechnology faces significant economic and market barriers

  2004cited by this paper
• PARSESNP: a tool for the analysis of nucleotide polymorphisms

  2003cited by this paper
• Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis.

  2003cited by this paper
• SIFT: predicting amino acid changes that affect protein function

  2003cited by this paper
• In vivo introduction of unpreferred synonymous codons into the Drosophila Adh gene results in reduced levels of ADH protein.

  2003cited by this paper
• Targeting induced local lesions IN genomes (TILLING) for plant functional genomics.

  2000cited by this paper
• The SELF-PRUNING gene of tomato regulates vegetative to reproductive switching of sympodial meristems and is the ortholog of CEN and TFL1.

  1998cited by this paper
• Genes directing flower development in Arabidopsis.

  1989cited by this paper
• The opaque-2 mutation of maize differentially reduces zein gene transcription.

  1989cited by this paper

• Harnessing the potential of mutation breeding, CRISPR genome editing, and beyond for sustainable agriculture

  2024cites this paper
• Spontaneous, Artificial, and Genome Editing-Mediated Mutations in Prunus

  2022cites this paper
• Mutagenesis for Targeted Breeding in Cotton

  2021cites this paper
• Improvements in Genomic Technologies: Applications to Crop Research

  2021cites this paper
• Mutation Breeding in Ornamentals

  2021cites this paper
• Introduction to Plant Mutation Breeding: Different Approaches and Mutagenic Agents

  2021cites this paper
• Effect of Hydrazine hydrate, tetracycline and streptomycin) on seed quality of tow hybrids of Zea mays L.( Nahrain and Furate )

  2021cites this paper
• Radiosensitivity of two varieties of watermelon (Citrullus lanatus) to different doses of gamma irradiation

  2020cites this paper
• Induced Mutation: Creating Genetic Diversity in Plants Induced Mutation: Creating Genetic Diversity in Plants

  2019cites this paper
• Variation Caused by Gamma Rays and Ethyl Methane Sulfonate on the Morphological Characters, Lycopene and Vitamin C Contents of Tomato

  2018cites this paper
• Efectos de agentes mutagénicos en la germinación de semillas de aguaymanto

  2018cites this paper
• Induced Mutation: Creating Genetic Diversity in Plants

  2018cites this paper
• Determination of effective mutagen dose for lettuce (Lactuca sativa var. longifolia cv. cervantes) seeds

  2017cites this paper
• Enhancing genetic gain in the era of molecular breeding

  2017cites this paper
• Mutant Resources and Mutagenomics in crop plants

  2017cites this paper
• Screening for pod shattering in mutant population of mungbean (Vigna radiata (L.) Wilczek)

  2017cites this paper
• Development, and genetic and metabolic characterization of new tomato mutants with enhanced and deficient carotenoid content

  2017cites this paper
• INDUCED MUTATIONS IN WHEAT IMPROVEMENTS ON NUTRITIONAL QUALITY OF THE GRAIN

  2017cites this paper
• Effect of Chemical Mutagens on Some Morphological Traits of Vicia faba L. Cv. Aqadulce

  2015cites this paper
• Induced Mutations and Crop Improvement

  2015cites this paper
• Induced Mutation in Sweet Potato aimed at Improved Quality and Drought Adaptation

  2014cites this paper
• Progress in TILLING as a tool for functional genomics and improvement of crops.

  2014cites this paper
• Optimization of EMS Mutagenesis on Petunia for TILLING

  2014cites this paper
• 100 Gy 60Co γ-Ray Induced Novel Mutations in Tetraploid Wheat

  2014cites this paper
• Mutagenesis—A Potential Approach for Crop Improvement

  2013cites this paper
• Comparative study between the effect of different concentrations and duration of soaking in EMS on germination and seedling growth of Okra Abemoschus esculenthus (L). Moench)

  year unknowncites this paper