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Sex discrimination of Buloke (Allocasuarina luehmannii) for selective revegetation

M. Conomikes,Magali Wright,John Delpratt

Published 2011 in Muelleria

ABSTRACT

Allocasuarina luehmannii (Buloke) is a slow-growing dioecious species occurring primarily in south-eastern Australia. Larger seed-bearing female trees, at least 100 yrs old, are an essential food source for the threatened Calyptorhynchus banksii graptogyne (Karak). Sex-discrimination in Buloke was investigated utilising Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) techniques. While described as a dioecious species, 18% of the plants sampled were monoecious suggesting that this is in fact a subdioecious species. Stem material from 55 paddock trees was collected to identify genetic differences between female, male and monoecious plants. An initial screening of 56 primers, on a subset of five female, five male and five monoecious samples, identified 21 RAPD and 21 ISSR primers with clear scorable bands, but no unique female bands were amplified. Investigation into the sex determination mechanism of this species and the possible effect of environmental factors on sex expression is recommended before further sex-specific marker research is undertaken.

PUBLICATION RECORD

  • Publication year

    2011

  • Venue

    Muelleria

  • Publication date

    Unknown publication date

  • Fields of study

    Biology, Environmental Science

  • Identifiers
    DOI 10.5962/p.292517
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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